| Project/Area Number |
10680156
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
食生活
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| Research Institution | Kurashiki University of Science and the Atrs |
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Principal Investigator |
SUMI Hiroyuki Kurashiki University of Science and the Atrs, College of Science and Industrial Technology, Professor, 産業科学技術学部, 教授 (00107814)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | mushroom / Pleurotus ostreatus / platelet aggregation / ADP / fibrinolytic enzyme |
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| Research Abstract |
Oyster fungus (Pleurotus ostreatus) had strong activity for blocking of the aggregation of human platelets (220 × 10^6/ml) caused by ADP, collagen or thrombin. Active substance (named ASK-1) treated with Aspergillus/Rhizopus enzyme for 24 hrs at pH 4.5 could be easely extracted with water. After stopping the reaction by heating at 100 ℃ for 5 min, and then membrane filtering and lyophilizing, approximately 27 g of dark yellow powder were obtained from 1.7 kg mushroom. The IC_<50> value calculated with human PRP and ADP for the inducer was about 11.6-27.1 mg/ml.
By oral administration, ASK-1 showed strong inhibition for platelet aggregation in blood of both rats and humans. When administerd 500 mg/kg body in 6 healthy volunteers of 18-37 years, the platelet aggregation was decreased from 65±8% to 50± 13% using 10 μ M ADP for the inducer. Whereas the fibrinolytic activity determined by EFA and tPA were increased. By using zymography coupled with fibrinolysis accelerating substance (FAS), the activated fibrinolytic enzyme released from human cell was found to be tPA type plasminogen activator with molecular weight of about 70,000.
Oyster fungus had strong SOD and anti-oxidative activities. Values of 958±402 CU SOD activity/g (dry weight) determined by nitrite method was higher than that of Natto reported previously. The concentrated fraction using Celite 545 column had also anti-oxidative activity against 2-deoxylibose. After ingestion of this fraction in healthy human volunteers, both strong inhibitions for apetite and loss of weight were proved.
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