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Community analysis of microorganisms metabolizing multiple aromatic compounds in soil

Research Project

Project/Area Number 10680542
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 環境保全
Research InstitutionNAGOYA UNIVERSITY

Principal Investigator

KATAYAMA Arata  Nagoya University, Graduate School of Bioagricultural Sciences, Associate Professor, 大学院・生命農学研究科, 助教授 (60185808)

Co-Investigator(Kenkyū-buntansha) WATANABE Akira  Nagoya University, Graduate School of Bioagricultural Sciences, Associate Professor, 大学院・生命農学研究科, 助教授 (50231098)
豊田 剛己  名古屋大学, 農学部・助教授, 助手 (30262893)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥2,800,000 (Direct Cost: ¥2,800,000)
Keywordsaromatic compounds / microbial degradation / dehydrogenase / dechlorination / quinone profile / ィイD114ィエD1C quinone / soil / multiple substrates / ^<14>C標識化合物 / 部分阻害法 / 分解菌検出
Research Abstract

This study conducted to develop the methods to measure (1) the profile of microbial population degrading aromatic compounds i soil, (2) the microbial groups metabolizing a single substrate in soil using ィイD114ィエD1C quinone profile analysis and (3) the microbial groups metabolizing multiple substrates in soil using ィイD114ィエD1C quinone profile analysis. (1) The profile of microbial population degrading aromatic compounds were determined by using indicator of dehydrogenase activity of microorganisms degrading aromatic compounds and by coloring reaction of chloride anion released from chloroaromatic compounds. The results suggested that application of farmyard manure into soil increase the microbial population to degrade aromatic compounds in soil. The population degrading, 2,4-dichlorophenol in soil was increased by application of farmyard manure but not that degrading 3-chlorophenol. (2) Using glucose, glycine and acetate, ィイD114ィエD1C quinone profile method was developed to detect the microbial groups degrading a single substrate. The reproducibility was high so that the statistical analysis of active microbial community between the soils examined. (3) Phenol and benzoate were used as model aromatic compounds. The ィイD114ィエD1C quinone profile method was used as model aromatic metabolizing phenol in the presence of benzoate and vice versa. Mineralization rates of aromatic compounds and total quinone profiles and labeling rates of quinones were almost identical between the duplicated experiment but the ィイD114ィエD1C quinone profiles were completely different. This suggested that the microenvironment in soil affected the microbial groups metabolizing aromatic compounds in soil.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Saitou, Fujie and Katayama: "Detection of microbial groups metabolizing a substrate in soil by the [14C]quinone"Soil Sci. Plant Nutr.. 45. 669-679 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Uchida et al.: "Changes in microbial communit structure in soil percolated with pentachlorophenol"J. Pesticide Sci.. 24. 186-188 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hu et al.: "Quantitative analysis of microfloral changes in a bioreactor for waste water…"Water Research. 33. 3263-3270 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Saitou et al.: "Linear relation between the amount of respiratory quinones and the microbial…"Soil Sci. Plant Nutr.. 45. 775-778 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Watanabe et al.: "Evaluation of orgins of CH4 carbon emitted from rice paddies"J. Geophys. Res.. 104(D19). 23623-23629 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toyota et al.: "Impact of fumigation with metam sodium upon soil microbial community structure"Soil Sci. Plant Nut.. 45. 207-223 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 片山新太、山本広基: "新 土と微生物(4) 環境と微生物 第2章 農薬と微生物"博友社. 7 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Uchida, S. Tanaka, H., Fujie, K. and Katayama, A.: "Changes in microbial community structure in soil percolated with pentachlorophenol with reference to respinatory quinone profile"J. Pesticide Sci.. 24. 186-188 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hu, H. -y., Fujie, K., Nakagome, H., Urano, K. and Katayama, A.: "Quantitative analysis of microfloral changes in a bioreactor for waste water treatment using novel indices of diversity and equitability of respiratory quiones"Water Research. 33. 3263-3270 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Saitou, K., Fujie, K. and Katayama, A.: "Detection of microbial groups metabolizing a substrate in soil by the [14C] quinone profile"Soil Sci. Plant Nutr. 45. 669-679 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Saitou, K. Nagasaki, K. Yamakawa, H. Hu, H-.Y., Fujie, K. And Katayama, A.: "Linear relation between the amount of respiratory quinones and the microbial biomass in soil"Soil Sci. Prant Nutr.. 45. (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Watanabe, A, Takeda, T., and Kimura, M.: "Evaluation of origins of CHィイD24ィエD2 carbon emitted from rice paddies"J. Geophys Res.. 104(D19). 23623-23629 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Toyota, K., Ritz, K., Kuninaga, S. and Kimura, M.: "Impact of fumigation with metam sodium upon soil microbial community structure in two Japanese soils"Soil Sci. Plant Nutr.. 45. 207-223 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Katayama, A. and Yamamoto, H.: "Chapter 2 Pesticides and microorganisms""New series of microorganismsin soil No. 4, Microorganisms and Environmental Problems" Ed by Japanese Society of Soil microorganisms, Hakuyusha (in Japanese). 29 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Saitou,Fujie and Katayama: "Detection of microbial groups metabolizing a substrate in soil by the [14C]quinone"Soil Sci.Plant Nutr.. 45. 669-679 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Uchida.et al: "Changes in microbial community structure in soil percolated with pentachlorophenol"J.Pesticide Sci.. 24. 186-188 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Hu et al: "Quantitative analysis microfloral changes in a bioreactor waste water …"Water Research. 33. 3263-3270 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Saitou et al: "Linear relation between the amount of respiratory quinones and the microbial…"Soil Sci Plant Nutr. 45. 775-778 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Watanabe et al: "Evaluation of origins of CH4 carbon emitted from rice paddies"J.Geophys.Res.. 104(D19). 23623-23629 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toyota et al: "Impact of fumigation with metam sodium upon soil microbial community structure"Soil Sci Plant Nut.. 45. 207-223 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 片山新太 山本広基: "「新 土と微生物 (4) 環境と微生物」 第2章 農薬と微生物"博友社. 73-79 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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