| Project/Area Number |
10680588
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kitasato University |
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Principal Investigator |
ISHII Ikuko Kitasato Univ., School of Medicine, Research Associate, 医学部, 助手 (00184458)
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| Co-Investigator(Kenkyū-buntansha) |
IWASE Hitoo Kitasato Univ., School of Medicine, Associate Professor, 医学部, 助教授 (60050663)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | glycoprotein / oligosaccharide / conformation / sugar chain |
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| Research Abstract |
The conformation of sugar chain in aqueous solution which constituted the glycoprotein was analyzed using magnetic anisotropy effect of the ィイD11ィエD1H-NMR spectrum in order to clarify the physiological function of the glycoprotein. Since there is no example applied in the field of the sugar chain analysis, this method developed the basic methodology. As a model compound, pyridylaminated sugars of O-linked glycoprotein core region were used.
1. These Galβ1-3GalNAc-PA and Galβ1-3GalNAc-o1 were purified from the O-linked glycoproteins. Then, 600MHz-ィイD11ィエD1H-NMR spectra of these samples were measured.
2. All protons of Galβ1-3GalNAc-PA and Galβ1-3GalNAc-o1 were analyzed. This analysis was carried out by spectral simulation method using the LAOCOON III program.
3. Conformation analysis of the pyranose ring of the part of Galβ was carried out.
4. In comparison with aliphatic protons of Galβ1-3GalNAc-PA and Galβ1-3GaNAc-o1, the protons which were shifting high magnetic field or low magnetic field were chosen. The protons in which magnetic anisotropy effect by the pyridine ring had appeared in those protons were chosen.
5. The possible conformers of Galβ1-3GalNAc-PA were generated by the Monte Carlo method using the molecular mechanics calculation (MM2).
6. From generated conformers, by selecting the structure which agrees with the result of the ィイD11ィエD1H-NMR spectrum, the conformational structure was decided.
At present, the more complicated oligosaccharides are analyzed.
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