| Project/Area Number |
10680603
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Nagaoka University of Technology |
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Principal Investigator |
YAMADA Ryo-hei Nagaoka University of Technology, Department of Environmental and Civil Engineering, Professor, 工学部, 教授 (00079708)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | N-methyl-D-aspartate / N-methyl-L-aspartate / D-aspartate / Scapharca broughtoni / Tapes japonica / Corbicula sandai / N-methyl-D-glutamate / サルボウガイ / ハマグリ / Scapharca |
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| Research Abstract |
(1) We developed a method of determination of N-methyl-D-aspartate by high-performance liquid chromatography that includes deivatization of the compound with (+)-1-(9-fluorenyl) ethyl chloroformate after treating biological extracts with ο-phthalaldehyde and N-acetyl-L-cysteine.
(2) Considerable amounts of N-methyl-D-aspartate were found in the adductor, gill and mantle of Scapharca broughtonii in addition to the foot muscle that had been known to contain the compound.
(3) N-Methyl-D-aspartate was found in bivalves such as Scapharca subcrenata, Mertrix lusoria, Patinopecten yessoensis, Crassostrea nippona and Mytilus edulis, but not in other organisms examined including various vertibrates and invertibrates.
(4) N-Methyl-L-aspartate was found in Tapes japonica and Corbicula sandal which do not contain N-methyl-D-aspartate.
(5) A compound, probably N-methyl-D-glutamate, was found in Scapharca broughtonii, and is on the way of identification.
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