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Analysis of the structure and interaction of apoptotic signaling molecules via Fas

Research Project

Project/Area Number 10680606
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

SAKAMAKI Kazuhiro  Kyoto University, Institute for Virus Research Associate Professor, ウイルス研究所, 助教授 (20271017)

Co-Investigator(Kenkyū-buntansha) YONEHARA Shin  Kyoto University, Institute for Virus Research Professor, ウイルス研究所, 教授 (00124503)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥2,700,000 (Direct Cost: ¥2,700,000)
Keywordsapoptosis / Death Effector Domain / Caspase-8 / FADD / MC159 / デス・エフェクター・ドメイン / デス-エフェクター・ドメイン / Caspase-8
Research Abstract

The death effector domain(DED), which is essential for protein interaction, is involved in apoptotic signal transudation. Cysteine protease caspase-8 and viral protein MC159 have two tandemly repeated DEDs, respectively. The DEDs of caspase-8 could induce apoptosis whereas that of MC159 inhibits apoptosis. To examine binding and biological activities of the DED domains, we generated deletion and chimera mutants of DEDs derived from caspase-8 and MC159. These mutants did not work compare to wild type of caspase-8 and MC159. Therefore, we concluded that the combination of the two-repeated DEDs is necessary for the DED-containing proteins to stimulate or inhibit apoptosis.
By searching DED-containing proteins on Genbank database, we found a new molecule which contains two tandemly repeated DEDs at the amino-terminal portion. We detected high expression of this molecules in the mouse testis by northern blot analysis. We suppose that this molecule may have a new function for signal transduction.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] Yonehara, Shin: "Requirement of cooperative functions of two repeated death effector domains in caspase-8 and in MC159 for induction and inhibition of apoptosis, respectively."Genes to Cells. 4. 541-549 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Sakamaki, Kazuhiro: "Molecular cloning and characterization of mouse caspase-8"European Journal of Biochemistry. 253. 399-405 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shin-ichi Tsukumo and Shin Yonehara: "Requirement of cooperative functions of two repeated death effector domains in caspase-8 and in MC159 for induction and inhibition apoptosis, respectively."Genes to Cells. 4. 541-549 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Kazuhiro Sakamaki et al: "Molecular cloning and characterization of mouse caspase-8."European Journal of Biochemistry. 253. 399-405 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] S.Tsukumo and S.Yonekura: "Requirement of cooperative functions of two repeated death effector domains in caspase-8 and in MC159 for induction of apoptosis,respectively"Genes Cells. 4. 541-549 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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