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Computed Tomography using projection X-ray microscope for Biological materials

Research Project

Project/Area Number 10680643
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionMeiji University

Principal Investigator

YOSHIMURA Hideyuki  Meiji University, School of Science and Technology, Professor, 理工学部, 教授 (70281441)

Co-Investigator(Kenkyū-buntansha) ONO Osamu  Meiji University, School of Science and Technology, Professor, 理工学部, 教授 (50130802)
ARAKAWA Kaoru  Meiji University, School of Science and Technology, Professor, 理工学部, 教授 (30183734)
TAKENO Jyunichi  Meiji University, School of Science and Technology, Professor, 理工学部, 教授 (90130822)
Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2000: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsPROJECTION X-RAY MICROSCOPE / DOMPUTED TOMOGRAPHY / CT / THREE-DIMENSIONAL RECONSTRUCTION / CRITICAL POINT DRYING / Omiscus Porcellio / ワラジムシ / メダカ幼魚 / コンピュータトモグラフィー / 臨界点乾燥法 / 蟻 / 立体構造
Research Abstract

The projection X-ray microscope makes use of a very small X-ray source from a thin (0.1-3 μm) target metal film that is excited by the focused electron beam of a scanning electron microscope (SEM). If an object is placed just below the target metal film, the diverging X-rays enlarge the shadow of the object. Because the size of X-ray light source is less than 100nm, the resolution of this microscope is about 0.1μm which is better than that of optical microscopes. We have applied this microscope for three-dimensional (3-D) structure analysis by means of cone-beam computerized tomography. The projection images of small insect, Omiscus Porcellio, were recorded in every 1° for whole direction (360°) with a stepping motor controlled sample rotator. An Omiscus Porcellio was prepared by critical point drying method and fixed on a thin adhesive tape (0.4μm thick) of the sample rotator. The images were recorded with cooled CCD camera (HAMAMATSU C4880) which detect X-ray directly. The 3-D image was reconstructed from corn-beam projections using back-projection algorithm. The resolution of the reconstructed 3-D image (256 x 256 x 256) was about 3 μm. The digestive organs were clearly visualized in 3-D.We are going to analyze smaller objects such as nerve networks which contains more detailed structure.

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Hideyuki Yoshimura, et.al.: "Application of SEM-modified X-ray microscope to entomology and histology, and effects of X-ray coherence in imaging."J.Electron Microscopy. 49(5). 621-628 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sinnya Kumagai, et.al.: "X-ray Microscopy and Spectroscopy, Internal Structures of Small Animals and Tissues"Jurgen Thieme et.al., Springer-Verlag. 273-278 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kunio Shinohara, et.al.: "X-ray Microscopy, Development of an X-ray Projection Microscope Using Synchrotron Radiation"Werner Meyer-IIse et.al., American Institute of Physics. 748(346-349) (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshimasa Yamamoto, et.al.: "X-ray Microscopy, Fine Surface Structure of Unfixed and Hydrated Marcrophages Observed by Laser-Plasma X-ray Contact Microscopy"Werner Meyer-IIse et.al., American Institute of Physics. 748(174-177) (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hideyuki Yoshimura, et.al.: "X-ray Microscopy, Contrast Enhancement and Three-Dimensional Computed Tomography in Projection X-ray Microscopy"Werner Meyer-IIse et.al., American Institute of Physics. 748(525-528) (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Osamu Ono, et.al.: "Proc.of Japan-USA Sympo.on Flexible Automation 2000 Real-time Recognition of 3D Structures by Using Chaotic Random Image Sequence2000"JUFSA. 1-4 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Yoshimura, D.Shoutsu, T.Horikoshi, H.Chiba, S.Kumagai, K.Takahashi, T.Mitsui: "Application of SEM-modified X-ray Microscope to Entomology and Histology, and Effects of X-ray Coherence in Imaging"J.Electron Micros. 49. 621-628 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] O.Ono, F.Ohkita, J.Takeno: "Real-time Recognition of 3D Structures by Using Chaotic Random Image Sequence"Proc.of Japan-USA Sympo.on Flexible Automation 2000 (ASME) JUFSA 2000-13079. 1-4 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kumagi, T.Horikoshi, H.Chiba, K.Taskahashi, D.Shoutsu, H.Yoshimura, T.Mitsui: "Internal Structures of Small Animals and Tissues X-ray Microscopy and Spectroscopy"Springer-Verlag. 273-278 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Shinohara, A.Ito, T.Honda, H.Yoshimura, K.Yada: "Development of an X-ray Projection Microscope Using Synchrotron Radiation, X-ray Microscopy"American Institute of Physics. 346-349 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Y.Yamamoto, H.Friedman, H.Yoshimura, Y.Kinjyo, S.Shioda, K.Debari, K.Shinohara, J.Rajyaruru, M.Richardson: "Fine Surface Structure of Unfixed and Hydrated Marcrophages Observed by Laser-Plasma X-ray Contact Microscopy X-ray Microscopy"American Institute of Physics. 174-177 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Yoshimura, D.Shoutsu, C.Kuzuryu, C.Miyata, R.Sano, T.Obi, N.Ohyama: "Contrast Enhancement and Three-Dimensional Computed Tomography in Projection X-ray Microscopy, X-ray Microscopy"American Institute of Physics. 525-528 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hideyuki Yoshimura, et. al.: "Application of SEM-modified X-ray microscope to entomology and histology, and effects of X-ray coherence in imaging "J.Electron Microscopy. 49(5). 621-628 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hideyuki Yoshimura,et.al.: "Contrast Enhancement and Three-dimensional Computed Tomography in Projection X-ray Microscopy"X-ray Microscopy and Spectroscopy. 未定. (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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