Computed Tomography using projection X-ray microscope for Biological materials
| Project/Area Number |
10680643
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Meiji University |
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Principal Investigator |
YOSHIMURA Hideyuki Meiji University, School of Science and Technology, Professor, 理工学部, 教授 (70281441)
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| Co-Investigator(Kenkyū-buntansha) |
ONO Osamu Meiji University, School of Science and Technology, Professor, 理工学部, 教授 (50130802)
ARAKAWA Kaoru Meiji University, School of Science and Technology, Professor, 理工学部, 教授 (30183734)
TAKENO Jyunichi Meiji University, School of Science and Technology, Professor, 理工学部, 教授 (90130822)
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| Project Period (FY) |
1998 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2000: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | PROJECTION X-RAY MICROSCOPE / DOMPUTED TOMOGRAPHY / CT / THREE-DIMENSIONAL RECONSTRUCTION / CRITICAL POINT DRYING / Omiscus Porcellio / ワラジムシ / メダカ幼魚 / コンピュータトモグラフィー / 臨界点乾燥法 / 蟻 / 立体構造 |
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| Research Abstract |
The projection X-ray microscope makes use of a very small X-ray source from a thin (0.1-3 μm) target metal film that is excited by the focused electron beam of a scanning electron microscope (SEM). If an object is placed just below the target metal film, the diverging X-rays enlarge the shadow of the object. Because the size of X-ray light source is less than 100nm, the resolution of this microscope is about 0.1μm which is better than that of optical microscopes. We have applied this microscope for three-dimensional (3-D) structure analysis by means of cone-beam computerized tomography. The projection images of small insect, Omiscus Porcellio, were recorded in every 1° for whole direction (360°) with a stepping motor controlled sample rotator. An Omiscus Porcellio was prepared by critical point drying method and fixed on a thin adhesive tape (0.4μm thick) of the sample rotator. The images were recorded with cooled CCD camera (HAMAMATSU C4880) which detect X-ray directly. The 3-D image was reconstructed from corn-beam projections using back-projection algorithm. The resolution of the reconstructed 3-D image (256 x 256 x 256) was about 3 μm. The digestive organs were clearly visualized in 3-D.We are going to analyze smaller objects such as nerve networks which contains more detailed structure.
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Report
(4 results)
Research Products
(14 results)
