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Molecular analysis of control mechanisms for meiosis.

Research Project

Project/Area Number 10680646
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionTohoku University

Principal Investigator

HIGASHITANI Atsushi  Tohoku University, Institute of Genetic Ecology, Associate Professor, 遺伝生態研究センター, 助教授 (40212162)

Project Period (FY) 1998 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1998: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsMeiosis / Synapses / Meiotic recombination / Sterile / Barley / High temperature injury / DNA repair / Caenorhabditis elegans / 花粉母細胞 / 相同的遺伝子組換え / 蛋白質リン酸化 / キナーゼ / 放射線感受性 / meiosis / recombination / check point control / recA / rad51 / dmcL / lim15 / atm
Research Abstract

In this study, we have carried out characterizations of a recA-like, gene Ce-rdh-1, ATM (ataxia telangiectasia mutated gene)-like gene Ce-atl-1 and Chk2 (check point gene 2)-like gene Ce-chk-2 from the nematode Caenorhabditis elegans. Ce-rdh-1 gene was essential for meiotic recombination and for repair of DNA double-strand breaks (Takanami et al., 1998, 2000). Ce-chk-2 gene encoded an essential protein kinase to form meiotic chromosome synapses. The repression of the Ce-chk-2 gene expression resulted in the abnormal and unpaired chromosomes of pachytene nuclei, the loss of meiotic recombination and the phenotype of achiasmata of diplotene and diakinesis nuclei. However, Ce-chk-2 gene was dispensable for repair process of DNA double-strand breaks (Higashitani et al., 2000). On the contrary, Ce-atl-1 gene was necessary for DNA repair process and the sensitivity to UV irradiation was increased by the repression of Ce-atl-1 gene. The repression of Ce-atl-1 gene also increased the incidence of somatic mutations, the extensive embryonic lethality, and the aneuploidy and chromosome instability during mitosis and meiosis. It indicates that Ce-atl-1 acts as a monitoring enzyme for DNA damage checkpoint control (Aoki et al., 2000).
We have also carried out characterization of the effects of high-temperature stress on the development of pollen mother cells (PMCs) and microspores in barley plant (Hordeum vulgare L). When plants were exposed to high temperature for five days at the pre-meiotic stage, PMCs and tapetum cells failed to develop. The panicles at the heading stage had a normal appearance, but no pollen grains in their abnormal anthers were observed and their seeds were virtually sterile (Sakata et al., 2000).

Report

(4 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Higashirani,A.et al.,: "Caenorhabditis elegans Chk2-like gene is essential for meiosis but dispensable for DNA repair."FEBS Letters. 485. 35-39 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takanami,T. et al.,: "Hyper-resistance of meiotic cells to radiation due to a strong expression of a single recA-like gene in Caenorhabditis elegans."Nucleic Acids Research. 28. 4232-4236 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Aoki,H. et al.,: "Characterization of Ce-atl-1, an ATM-like gene from Caenorhabditis elegans."Molecular Genenal Genetics. 264. 119-126 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sakata,T. et al.,: "Effects of high temperature on the development of pollen mother cells and microspores in barley Hordeum vulgare L."Journal Plant Research. 113. 395-402 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takanami,T. et al.,: "Characterization of a Caenorhabditis elegans recA-like gene Ce-rdh-1 involved in meiotic recombination."DNA Research. 5. 373-377 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Atsushi Higashitani, Hidetoshi Aoki, Akiyuki Mori, Yohei Sasagawa, Takako Takanami, Hideyuki Takahashi: "Caenorhabditis elegans Chk2-like gene is essential for meiosis but dispensable for DNA repair."FEBS Letters. 485. 35-39 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takako Takanami, Akiyuki Mori, Hideyuki Takahashi, Atsushi Higashitani: "Hyper-resistance of meiotic cells to radiation due to a strong expression of a single recA-like gene in Caenorhabditis elegans."Nucleic Acids Research. 28. 4232-4236 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hidetoshi Aoki, Shusei Sato, Takako Takanami, Takeshi Ishihara, Isao Katsura, Hideyuki Takahashi, Atsushi Higashitani: "Characterization of Ce-atl-1, an ATM-like gene from Caenorhabditis elegans."Molecular General Genetics. 264. 119-126 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tadashi Sakata, Hideyuki Takahashi, Iwao Nishiyama, Atsushi Higashitani: "Effects of high temperature on the development of pollen mother cells and microspores in barley Hordeum vulgare L."Journal Plant Research. 113. 395-402 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takako Takanami, Shusei Sato, Takeshi Ishihara, Isao Katsura, Hideyuki Takahashi, Atsushi Higashitani: "Characterization of a Caenorhabditis elegans recA-like gene Ce-rdh-1 involved in meiotic recombination"DNA Research. 5. 373-377 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Higashitani,A. et al.,: "Caenorhabditis elegans Chk-2-like gene is essential for meiosis but dispensable for DNA repair."FEBS Letters. 485. 35-39 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takanami,T. et al.,: "Hyper-resistance of meiotic cells to radiation due to a strong expression of a single recA-like gene in Caenorhabditis elegans."Nucleic Acids Res.. 28. 4232-4236 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Aoki,H. et al.,: "Characterization of Ce-atl-1, an ATM-like gene from Caenorhabditis elegans."Molecular General Genet.. 264. 119-126 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sakata,T. et al.,: "Effects of high temperature on the development of pollen mother cells and microspores in barley Hordeum vulgare L."J.Plant Res.. 113. 395-402 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Endo,M. et al.,: "Analysis of expressed sequence tags of flower buds in Lotus japonicus."DNA Res.. 7. 213-216 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takanami, T. et al.,: "Characterization of a Caenorhabditis elegans recA-like gene Ce-rdh-1 involved in meiotic recombination."DNA Res.. 5. 373-377 (1998)

    • Related Report
      2000 Annual Research Report
  • [Publications] Asano S.et al.: "Filamentous phage replication initiator protein gpII forms a covalent complex with the 5'end of the nick introduced"Nucleic Acids Res.. 27. 1882-1889 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takanami,T.et al: "Characterization of a Caenorhabditis elegans recA-like gene Ce-rdh-f involved in meiotic recombination." DNA Research. 5,. 373-377 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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