| Project/Area Number |
10680648
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KAWAKAMI Koichi The University of Tokyo, The Institute of Medical Science, Assistant Professor, 医科学研究所, 助手 (70195048)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Zebrafish / Medaka fish / Transposon / Transgenesis / Transgenic / Reverse genetics / Microinjection / 分子生物学 / 発生遺伝学 / トランスジェニックアニマル |
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| Research Abstract |
Zebrafish is a powerful model animal to study functions of vertebrate genes that regulate complex biological phenomena. In order to increase the usefulness of this model animal, we aim to develop novel reverse genetics technologies which will allow us to study the function of cloned genes. Especially, in this study, we aim to develop a novel transgenesis technology using the Tol2 element, an Ac-like transposable element of the Japanese medaka fish. I successfully obtained the results as described below.
1. The Tol2 element encodes a functional transposase that catalyzes its excision in zebrafish embryos .
2. The Tol2 element encodes a functional transposase that catalyzes its transposition in the zebrafishlineage.
3. The zebrafish hagoromo gene is an ortholog of the mouse Dactylin gene and is involved in pigment pattern formation in adult fish.
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