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The analysis of the function and establishment mechnism of paused polymerase

Research Project

Project/Area Number 10680650
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Molecular biology
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

HIRAYOSHI Kazunori  Kyoto Univ., Inst. for Frontier Medical Sciences, Lecturer, 再生医科学研究所, 講師 (80199108)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥2,100,000 (Direct Cost: ¥2,100,000)
KeywordsTranscriptional regulation / Drosophila / RNA polymerasell / Paused polymerase / HSP70 / TATA / DPE / GAGA / GAGA因子
Research Abstract

In Drosophila cell, an RNA polymerase is present on the 5' end of the uninduced hsp 70 gene and is transcriptionaly engaged, but paused. The polymerase has ready access to this uninduced gene and can initiate transcription, but is impeded from progressing beyond early elongation. To analyze the biological role of the paused polymerase, we developed the model system. The results showed 1. the possibility of the function of paused polymerase for quick activation of the gene. 2.GAGA factor, a general transcription factor found to interact with GA・CT repeats present in Drosophila is capable of binding to heat shock promoter sequences under non heat shock condition. GAGA factor is directly responsible for the displacement of histones from the heat shock promoter. In addition to the replacement of chromatin structure, we found the direct function as a transcription acrivator on the GAGA factor. 3. TATA, the main binding site of the TBP and TFIID binding site DPE(Down Promoter Element)are major elements for the establishment of the paused polymerase. Thesse results are suggested by the experiment with transient transfection with cultured cells. Now, we focus on the detail analyses of these element in vivo. Transgenic fly line will show the detail mechanism of tehese elements.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] A.Law, K.Hirayoshi, et al.: "Direct cloning of DNA that interacts in vivo with a specific protein : application to RNA polymeraseII and sites of pausing in Drosophiola"Nucleic Acids Research. 26. 919-924 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K.Hirayoshi and J.T.Lis: "Nuclear run-on assays : assessing trascription by mesuring density of engaged RNA precursors"Methods in Enzymol.. 304. 351-362 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] A. Law, K. Hirayoshi et al: "Direct cloning of DNA that interacts in vivo with a specific protein : application to RNA polymeraseII and sites of pausing in Drosophiola."Nucleic Acids Research. 26. 919-924 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K. Hirayoshi and J. T. Lis: "Nuclear run-on assays : assessing transcription by mesuring density of engaged RNA precursors."Methods in Enzymol.. 304. 351-362 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] K.Hirayoshi and J.T.Lis: "Nuclear run-on assays : ssessing transcription by mesuring density of egaged RNA precusors"Methods in Enzymol.. 305. 351-362 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Law A.,Hirayoshi K.,et al: "Direct cloning of DNA that interacis in vivo with a specific protein : application to RNA polymerascII and sites of pausing in Drospophiola." Nucleic Acids Research. 26(4). 919-924 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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