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Molecular Mechanism of Mitochondrial Protein Import and Sorting

Research Project

Project/Area Number 10680659
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionYAMAGATA UNIVERSITY

Principal Investigator

ONO Hideyu  School of Medicine, Yamagata University, Associate Professor, 医学部, 助教授 (40160915)

Co-Investigator(Kenkyū-buntansha) IUCHI Yoshihito  School of Medicine, Yamagata University, Research Assistant, 医学部, 助手 (60272069)
YOSHIDA Tadashi  School of Medicine, Yamagata University, Professor, 医学部, 教授 (10004673)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1999: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsmitochondria / protein targeting / protein translocation / mitochondrial outer membrane / mitochondrial inner membrane / プレシークエンス
Research Abstract

In the process of mitochondrial protein import, various cytosolic factors play an important role. We found new cytosolic factor in rat liver cytosol and this factor was insensitive to N-ethylmaleimide, suggesting that this was not completely different from MSF. We tried to purify this factor and partiually characterized. From our research, it is likely that this factor works in the process of recognition between mitochondorial protein precursor and mitochondirial surface.
We also examined import and shorting of coproporphyrinogen oxidase (CPO, mitochondrial intermembrane space enzyme). The presequence of CPO had both matrix targeting and sorting signal and the precursor was processed to the intermediate form before converting to mature form and localization to the intermembrane space. Two types of intermediates were observed. Matrix processing peptidase might form the two types of intermediates by cutting the two recognition site of the precursor. Moreover, we observed hemin inhibition of transport of the precursor into rat mitochondria, suggesting that import of the precursor was regulated by heme concentration.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (3 results)

All Other

All Publications (3 results)

  • [Publications] 諏佐真治: "ヒト・コプロポルフィリノーゲンオキシダーゼ前駆体のラット肝ミトコンドリア膜間区画への輸送反応"ポルフィリン. 8. 21-25 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Shinji Susa: "The Import of Human Coproporphyrinogen Oxidase to the intermembrane Space of Rat Mitochondria"Porphyrin. 8. 21-25 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 諏佐 真治: "ヒト・コプロポルフィリノーゲンオキシダーゼ前駆体のラット肝ミトコンドリア膜間区画への輸送反応"ポルフィリン. 8・1. 21-25 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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