| Project/Area Number |
10680706
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | OSAKA CITY UNIVERSITY |
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Principal Investigator |
MAEDA Mitsuyo (1999) Osaka City Univ. Med. Sch., Lectu., 医学部, 講師 (40122080)
高木 宏 (1998) 大阪市立大学, 医学部, 教授 (30163174)
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| Co-Investigator(Kenkyū-buntansha) |
YONEDA Takunari Osaka City Univ. Med. Sch., Assoc., 医学部, 助手 (70271179)
前田 光代 大阪市立大学, 医学部, 講師 (40122080)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,000,000 (Direct Cost: ¥3,000,000)
Fiscal Year 1999: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1998: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | Microglia / gene therapy / brain ischemia / GDNF / 遺伝子導入 |
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| Research Abstract |
A recombinant adenoviral vector encoding the human glial cell line-derived neurotrophic factor (GDNF) gene was used to express the neurotrophic factor GDNF in the lateral ventricle of gerbil brain before 2 days of transient bilateral common carotid artery occlusion. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick end labeling (TUNEL) staining in pyramidal cells of hippocampal CA-1 was markedly reduced in the case with GDNF treatment at 2, 4 and 7 days of reperfusion. In GDNF treated group, pyramidal cells were well preserved in the hippocampus at 7 days after reperfusion [7.1% vs. 62.8% for vehicle and GDNF, respectively]. This results suggest that the treatment with GDNF has a significant protective effect on delayed neuronal death of hippocampal CA-1.
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