| Project/Area Number |
10680710
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Tokai University |
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Principal Investigator |
SEKIGUCHI Masaki Tokai University, School of Medicine, Assistant Professor, 医学部, 講師 (50163100)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Hiroshi Akita University, School of Medicine, Professor, 医学部, 教授 (40151104)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | midbrain / cerebellum / olivary nucleus / wnt 1 / swaying mouse / immunohistochemistry / wnt-1 |
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| Research Abstract |
The murine mutation "swaying" is caused by a mutant allele of the Wnt1 gene (Wnt1ィイD1SWィエD1) which has a single base pair deletion that terminates translation of the Wnt1 protein, We have analyzed a series of pre and postnatal swaying mice to evaluate the developmental effects of the absence of Wnt1ィイD1SWィエD1. On embryonic day 14(E14), the cerebellum and midbrain are still absent in the swaying mouse, On postnatal day 0 (PO) the hemispheres are clearly seen, but the midline fusion to form the vermis does not occur until between P5 and P7. The border between the inferior and superior colliculi in the midbrain does not appear and positional and size abnormalities become recognizable on PO. Meanwhile, the anterior lobes of the cerebellar hemispheres appear to fuse with the midbrain around P5. During P10-P15, laminar abnormalities of the cerebellum become recognizable. Also, from P5, asymmetries in the size of the cerebellar hemispheres appear and are accompanied by asymmetries in the lamination of the inferior olivary nucleus.
These findings indicate that the abnormal development in the cerebellum and midbrain of the swaying mouse proceeds in an orderly way and, in particular, indicates that a) abnormal development of the cerebellum is secondary to development of the midbrain and b) asymmetric development of the inferior olive is secondary to asymmetric development of the cerebellum.
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