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Specific Inhibitory Effects of Hybrid Liposomes on the Growth of Various Tumor Cells

Research Project

Project/Area Number 10680811
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionKumamoto Institute of Technology

Principal Investigator

MATSUMOTO Yoko  Kumamoto Institute of Technology, Associate Professor, 工学部, 助教授 (00133562)

Co-Investigator(Kenkyū-buntansha) KANNO Akihiro  Panapharm Laboratories, Researcher, 研究員
UEOKA Ryuichi  Kumamoto Institute of Technology, Professor, 工学部, 教授 (70099076)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1998: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordslipsome / chemotherapy / fusion / apoptosis
Research Abstract

1. Inhibitory Effects of Hybrid Liposomes on the Growth of tumor Cells in vitro
The inhibitory effects of hybrid liposomes composed of lipids having a variety of head groups (zwitterionic L-α-dimyristoylphosphatidylcholine (DMPC), anionic L α-dimyristoylphosphatidyl glycerol (DMPG) and cationic 1, 2-dimyristoyl-3-trimethylammonium propane (DMTAP)) and polyoxyethylene (10) dodecyl ether (CィイD212ィエD2(EO)ィイD210ィエD2) on the growth of tumor cells (human lung carcinoma (RERF-LC-OK), human hepatoma (Hep-G2), human stomach tumor (GT3TKB)) in vitro were examined. It is attractive that the highly specific inhibitory effect of the hybrid liposomes composed of DMPG/10mol% (CィイD212ィエD2(EO)ィイD210ィエD2) On the growth of RERF-LC-OK and GT3TKB was obtained without any antitumor drugs.
2. Induction of Apoptosis Hybrid Liposomes
We examined antitumor mechanism of the hybrid liposomes composed of dimyristoylphosphatidylcholine and polyoxyethylenealkyl ether having inhibitory action toward the growth of tumor cells related to apoptosis. The induction of apoptosis by the hybrid liposomes in HL-60 cells was verified on the basis of agarose gel electrophoresis of DNA and flow cytometry analysis with the propidium iodide staining method. A fluorescence micrograph taken using fluorescein isothiocyanate as a fluorescence probe showed that the hybrid liposomes should fuse with the tumor cell membrane. After fusion, accumulation of the hybrid liposomes to tumor cell membrane was revealed on the basis of DNA analysis.
3. Chemotherapy with Hybrid Liposomes for Melanomatosis
A mouse model of lung carcinoma was established by intraperitoneal inoculate of Lewis lung carcinoma cells. The remarkably prolonged survival (480%) was attained by administering the hybrid liposomes to mice. This result suggests that the hybrid liposomes should be effective for the treatment of cancers. Furthermore, no toxicity of the hybrid liposomes was obtained in normal rats in vivo with no side effects.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Yoko Matsumoto: "Intracellular Responses of Hybrid Liposomes against Leukemia Cells Related to Apoptosis with Antitumor Activity"Chemistry Letters. 1999. 53-54 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 金納 明宏: "肺癌モデルマウスに対するハイブリッド型リポソームの治療効果"Drug Delivery System. 14. 37-42 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 松本 陽子: "温度感受性ハイブリッド型リポソームの創製と薬物送達システムへの応用"熊本工業大学研究報告. 24. 107-113 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 松本陽子: "ハイブリッド型リポソームの種々の腫瘍細胞に対する特異的増殖抑制効果"薬学雑誌. 119. 280-287 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoko Matsumoto: "Remarkably Enhanced Inhibitory Effects of Hybrid Liposomes on the Growth of Specific Tumor Cells"Bioorganic & Medicinal Chemistry Letters. 9. 1937-1940 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Akihiro Kanno: "Chemotherapy with Hybrid Liposomes for Melanomatosis"Biological & Pharmaceutical Bulletin. 22. 1013-1014 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoko Matsumoto: "Intracellular Responses of Hybrid Liposomes against Leukemia Cells Related to Apoptosis with Antitumor Activity"Chemistry Letters. 53-54 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Akihiro Kanno: "Therapeutic Effects of Hybrid Liposomes on mice Model of Lung Carcinoma"Drug Delivery System. 14. 37-42 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoko Matsumoto: "Temperature-Sensitive Hybrid Liposomes Coupled to Drug Delivery System"Bulletin of Kumamoto Institute of Technology. 24. 107-113 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoko Matsumoto: "Specific Inhibitory Effects of Hybrid Liposomes on the Growth of Various Tumor Cells"Yakugakuzassi. 199. 280-287 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoko Matsumoto: "Remarkably Enhanced Inhibitory Effects of Hybrid Liposomes on the Growth of Specific Tumor Cells"Bioorganic & Medicinal Chemistry Letters. 9. 1937-1940 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Akihiro Kanno: "Chemotherapy with Hybrid Liposomes for Melanomatosis"Biological & Pharmaceutical Bulletin. 22. 1013-1014 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yoko Matsumoto: "Intracellular Responses of Hybrid Liposomes agaainst Leukemia Cells Related to Apoptosis with Antitumor Activity"Chemistry Letters. 1999. 53-54 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 金納明宏: "肺癌モデルマウスに対するハイブリット型リポソームの治療効果"Drug Delivery System. 14・1. 37-42 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 松本陽子: "温度感受性ハイブリット型リポソームの創製と薬物送達システムへの応用"熊本工業大学研究報告. 24・1. 107-113 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 松本陽子: "ハイブリット型リポソームの種々の腫瘍細胞に対する特異的抗腫瘍効果"薬学雑誌. 119・4. 280-287 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yoko Matsumoto: "Remarkably Enhanced Inhibitory Effects of Hybrid Loposomes on the Growth of Specifid Tumor Cells"Bioorganic&Medicinal Chemistry Letters. 9・14. 1937-1940 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Akihiro Kanno: "Chemotherapy with Hybrid Liposomes for Melanomatosis"Biological&Pharmaceutical Bulletin. 22・9. 1013-1014 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 金納明宏: "担癌モデルマウスに対するハイプリッド型リポソームの治療効果" Drug Delivery System. 13・2. 101-105 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoko Matsumoto: "Intraccllular Rcsponses of Hybrid Liposomes against LeuKemia Cells Related to Apoptosis with Antitrumor Activity" Chemistry Letters. 1999. 53-54 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 金納明宏: "肺癌モデルマウスに対するハイブリッド型リポソームの治療効果" Drug Delivery System. 14・1. 37-42 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] Osamu Tanoue: "Steric-Control for the Enantioselective Hydrolysis of Amino Acid Esters with Membrane-bound Enzyme Model" Tetrahedron Letters. 40・11. 2129-2132 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 松本陽子: "温度感受性ハイブリッド型リポソームの創製と薬物送達システムへの応用" 熊本工業大学研究報告. 24・1. 107-113 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 松本陽子: "ハイブリッド型リポソームの種々の腫瘍細胞に対する特異的増殖抑制効果" 薬学雑誌. 119・12. 280-287 (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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