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異常ヘモグロビン症診断のルーチン化のための新技術の開発

Research Project

Project/Area Number 10771350
Research Category

Grant-in-Aid for Encouragement of Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Laboratory medicine
Research InstitutionOsaka Medical College

Principal Investigator

宮崎 彩子  大阪医科大学, 医学部, 助手 (20298772)

Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1999: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1998: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywords質量分析 / 異常ヘモグロビン / DNA解析 / サラセミア / ルーチン化
Research Abstract

最新の質量分析(MS)法、等電点電気泳動法及び遺伝子解析法を組み合わせ、迅速・正確な実用的診断法を確立した。本システムを用い、学内外より依頼のあった異常ヘモグロビン(Hb)症疑いの16検体を分析した結果、以下の異常ヘモグロビン5種15症例を同定した。
Hb Riyadh(β120Lys->Asn)
Hb Hamadan(β56Gly->Arg)
Hb J-Meerut(α120Ala->Glu)
Hb G-Szuhu(β80Asn->Lys)
新変異(β120Lys->Asn、80Asn->Lys)
また、逆相カラムRP-300とPartisil(ODS)による異常鎖の分離法を加え、さらに多種の変異の同定に対応できるようにした。

Report

(2 results)
  • 1999 Annual Research Report
  • 1998 Annual Research Report
  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] Miyazaki A,Nakanishi T,Kishikawa M,Nakagawa T,Shimizu A,Mawjood AHM,Imai K,Aoki Y & Kikuchi M.: "Compound heterozygosity for b+-thalassemia-31(A->G)and a variant with low oxygan affinity,Hb Sagami [bl39(H17)Asn->Thr]"Hemoglobin.. 23・3. 267-271 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T.Nakanishi,et al.: "Hb Sagami [β139(H17)Asn→Thr] : a new hemoglobin variant not detected by isoelectrofocusing and propan-2-ol test.was detected by electrospray ionization mass spectrometry" J.Mass Specrim.33 (4). 565-569 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] T.Nakanishi,et al.: "A new hemoglobin variant found during HbA1c measurement : Hb Hokusetsu [β52(D3)Asp→Gly]." Hemoglobin. 22 (4). 355-355 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] A.Miyazaki,et al.: "Compound heterozygosity for β^+-Thalassemia-31(A→G) and a variant with low oxygen affinity,Hb Sagami [β139(H17)Asn→Thr]" Hemoglobin. (in press). (1999)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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