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Cellular and molecular mechanisms of magnetic fields

Research Project

Project/Area Number 10837006
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 電磁場環境
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

MIYAKOSHI Junji  GRADUATE SCHOOL OF MEDICINE, KYOTO UNIVERSITY ASSOCIATE PROFESSOR, 医学研究科, 助教授 (70121572)

Co-Investigator(Kenkyū-buntansha) FUJIMORI Akira  GRADUATE SCHOOL OF MEDICINE, KYOTO UNIVERSITY RESEARCH ASSOCIATE, 医学研究科, 助手 (50314183)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 1999: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1998: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsMagnetic fields / Mutation induction / Mutation spectrum / Gene expression / NOR-1 / Signal transduction / 極低周波変動磁場 / CHO細胞 / ベンガラクトシダーゼ
Research Abstract

Exposure of cultured human ostosarcoma cells (Saos-LP-12) to high-density (400mT at 50Hz) extremely low frequency magnetic fields (ELFMF) induced mutations in the hypoxanthine-guanine phosphoribosyl transferase gene. Saos-LP-12 cells, which are isolated form parental Saos-2 cells and have a deletion in the coding region of the p53 gene, are introduced to the wild-type (wt) p53 expression plasmid (pOPRSVp53). The mutation in Saos-LP-12 cells was suppressed by expression of the introduced wt p53 gene during 400mT ELFMF exposure. No. marked difference in the mutation spectrum was observed among the treatments of ELFMF [p53(-)], ELFMF [P53(+)], and sham exposures. Our findings suggest that wt p53 has a function in suppression of DNA replication errors and/or in maintenance of genomic stability after high-density ELFMF exposure.
On the other hand, enhanced expression of neuron derived orphan receptor (NOR-1) gene was observed by exposure of Chinese hamster ovary K1 (CHO-K1) cells to ELFMF of 50Hz at 400mT, but not at 5mT. The enhanced expression, reaching the maximum at 6h, was transient and reduced to the control level after exposure to 400mT ELFMF for 24h. The NOR-1 expression induced by treatment with forskolin and TPA was further enhanced by the simultaneous treatment with 400mT ELFMF, in which the maximum response was at 3h. The NOR-1 expression by these treatments was induced more earlier than that by 400mT ELFMF alone. When cells were treated with an inhibitor of the protein kinase C (calphostin C or crocetin) and CaィイD12+ィエD1 entry blockers (nifedipin and dantrolen) during the 400mT ELFMF exposure, the enhanced NOR-1 expression was not observed. Exposure of CHO-K1 cells to the high-density 400mT ELFMF may affect the signal transduction in the cells, resulting in the enhanced NOR-1 gene expression.

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] J.Miyakoshi: "Inhibition of I-κBαphosphorylation at serine and tyrosine acts independenty on sensitization to DNA damaging agents in human glioma cells"British Journal of Cancer. 82. 28-33 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] J.Miyakoshi: "Suppression of heat-induced HSP-70 by simultaneous exposure ot 50mT Magnetic field"Life Sciences. 66. 1187-1196 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] J.Miyakoshi: "Decreased host-cell reactivation of UV-irradiated adenovirus in human Colon tumor cell lines that have normal ppost-UV survival"Photochemistry and Photobiology. 70. 217-227 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] J.Miyakoshi: "Intracellular generation on reactive oxygen species and DNA damage in Escherichia coli mutants exposed to electromagnetic fields and X-ray"Environmental Technology. 20. 45-51 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] J.Miyakoshi: "Effect of high-density extremely low frequency magnetic field on sister chromatid exchanges in mouse m5S cells"Mutation Research. 440. 189-194 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] J.Miyakoshi: "Long term exposure to a magnetic field (5mT at 60Hz) increases X-ray Induced mutations"Journal of Radiation Research. 40. 13-21 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Junji Miyakoshi and Kasumi Yagi: "Inhibition of I κ B-α phosphorylation at serine and tyrosine acts independently on sensitization to DNA damaging agents in human glioma cells."British Journal of Cancer. 82. 28-33 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Junji Miyakoshi, Yukihiro Mori, Hiroko Yaguchi, Guirong Ding and Akira Fujimori: "Suppression of heat-induced HSP-70 by simultaneous exposure to 50 mT magnetic field."Life Sciences. 66. 1187-1196 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Rufus. S. Day, III, Aghdass Rasouli-Nia, James, Meservy, Sibghat-Ullah Lari, Kelly Dobler, Shigeru Tsunoda, Junji Miyakoshi, and Hiraku Takebe: "Decreased host-cell reactivation of UV-irradiated adenovirus in human colon tumor cell lines that have normal post-UV survival."Photochemistry and Photobiology. 70. 217-227 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yusuke Yonezawa, Junji Miyakoshi, Hiraku Takebe and Hajime Nishioka: "Intracellular generation on reactive oxygen species and DNA damage in Escherichia coli mutants exposed to electromagnetic fields and X-ray."Environmental Technology. 20. 45-51 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Hiroko Yaguchi, Masami Yoshida, Yosuke Ejima and Junji Miyakoshi: "Effect of high-density extremely low frequency magnetic field on sister chromatid exchanges in mouse m5S cells."Mutation Research. 440. 189-194 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Junji Miyakoshi, Tasuhiro Koji, Tetsuji Wakasa and Hiraku Takebe: "Long term exposure to a magnetic field (5mT at 60Hz) increases X-ray-induced mutations."Journal of Radiation Research. 40. 13-21 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] J.Miyakoshi: "Inhibition of I-κBα Phosphorylation at serine and tyrosine acts independenty on sensitization to DNA damaging agents in human glioma cells"British Journal of Cancer. 82. 28-33 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] J.Miyakoshi: "Suppression of heat-induced HSP-70 by simultaneous exposure to 50mT Magnetic field"Life Science. 66. 1187-1196 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] J.Miyakoshi: "Decreased host-cell reactivation of UV-irradiated, adenovirus in human Colon tumor cell lines that have normal post-UV survival"Photochemistry and Photobiology. 70. 217-227 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] J.Miyakoshi: "lntracellular generation on reactive oxygen species and DNA damage in Escherichia coli mutants exposed to electromagnetic fields and X-ray"Environmental Technology. 20. 45-51 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] J.Miyakoshi: "Effect of high-density extremely low frequency magnetic field on sister chromatid exchanges in mouse m5S cells"Mutation Research. 440. 189-194 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] J.Miyakoshi: "Long term exposure to a magnetic field(5mT at 60Hz) increases X-ray-Induced mutations"Journal of Radiation Research. 40. 13-21 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] J.Miyakoshi: "Enhanced NOR-1 gene expression by exposure of Chinese hamster cells to high-density 50 Hz magnetic fields." Molecular and Cellular Biochemistry. 181. 191-195 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] J.Miyakoshi: "Suppression of high-density magnetic field (400 mT at 50 Hz)-induced mutations by wild-type p53 expression in human osteosarcoma cells." Biochemical and Biophysical Research Communications. 243. 579-584 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] J.Miyakoshi: "Intracellular generation on reactive oxygen species and DNA damage in Escherichia coli mutants exposed to electromagnetic fields and X-ray." Environmental Technology. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] J.Miyakoshi: "Effect of high-density extremely low frequency magnetic field on sister chromatid exhanges in mouse m5S cells." Mutation Reserach. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] J.Miyakoshi: "Long-term exposure to a magentic field (5 mT at 60 Hz) increases X-ray-induced mutations." Journal of Radiation Research. (in press).

    • Related Report
      1998 Annual Research Report
  • [Publications] 宮越順二: "環境と健康(II)" (池永満生、野村大成、森本兼曩編集)、へるす出版, 15 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] 宮越順二: "創造" 小学館, 13 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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