| Project/Area Number |
10837011
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
電磁場環境
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| Research Institution | The University of Tokushima |
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Principal Investigator |
HOUCHI Hitoshi The University of Tokushima, Pharmacy, Associate Professor, 医学部・附属病院, 助教授 (00219156)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIZUMI Masanori The University of Tokushima, Pharmacology, Assistant, 医学部, 助手 (60294667)
SHIMIZU Eiji The University of Tokushima, Third Internal Medicine, Assistant Professor, 医学部, 講師 (50187449)
IKEHARA Toshitaka The University of Tokushima, Physiology, Assistant Professor, 医学部, 講師 (40111033)
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| Project Period (FY) |
1998 – 1999
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| Project Status |
Completed (Fiscal Year 1999)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 1999: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1998: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | Magnetic Field / Intracellular Free Calcium / Bradykinin / Adrenal Chromaffin Cells |
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| Research Abstract |
In adrenal chromaffin cells, which are regarded as model catecholamine-containing neurons and are useful for studies on the mechanism of stimulus-secretion coupling, secretory stimulation causes an increase in the intracellular free CaィイD12+ィエD1 ([CaィイD12+ィエD1]i) level originating from intracellular pools and extracellular spaces, leading to catecholamine secretion and activation of catecholamine biosynthesis.
The effect of exposure to the time-varying strong magnetic field on bradykinin-stimulated mobilization of [CaィイD12+ィエD1]i in adrenal chromaffin cells was examined. It exposed to time-varying magnetic field changing between 0.07 and 1.7 T at an interval of 3 s on transient increase in [CaィイD12+ィエD1]i stimulated by bradykinin in the cells. Addition of bradykinin induced the increase in [CaィイD12+ィエD1]i Within a few minutes. The exposure to the magnetic field perfectly suppressed the increase in [CaィイD12+ィエD1]i in CaィイD12+ィエD1 free medium. The inhibition occurred for 15 min when the maximum magnetic flux density was more than 1.4 T. These results suggest that the exposure inhibits CaィイD12+ィエD1 release from intracellular CaィイD12+ィエD1 stores.
Thus, magnetic field may have influence on the mechanism of stimulus-secretion coupling in catecholamine-containing neurons.
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