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Dynamics of Muscle and Bone Cells when Mechanically Extended via a Magnetic Field

Research Project

Project/Area Number 10838021
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field リハビリテーション科学
Research InstitutionHiroshima University

Principal Investigator

YUGE Louis  Faculty of Medicine, Hiroshima University Research assistant, 医学部, 助手 (20263676)

Co-Investigator(Kenkyū-buntansha) KAWAMATA Seiichi  Faculty of Medicine, Hiroshima University Professor, 医学部, 教授 (30127641)
Project Period (FY) 1998 – 1999
Project Status Completed (Fiscal Year 1999)
Budget Amount *help
¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1999: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1998: ¥1,600,000 (Direct Cost: ¥1,600,000)
Keywordsmyoblasts / osteoblasts / magnetic microparticles / magnetic field / mechanical stretch / cytoskeleton / cell differentiation / 分化マーカー / magnetic microparticle / 微小磁性体 / 機械的伸張
Research Abstract

We developed a new cell stimulation method in which magnetic microparticles (MPs) were introduced into the cytoplasm of cultured myoblasts or osteoblasts, and the cells were cultured in a magnetic field. The differentiation of myoblasts was examined from the viewpoint of their morphology and myogenin production. After exposure to the magnetic field, the cells containing MPs became larger and were elongated along the axis of the magnetic poles. Myogenin, a muscle specific regulatory factor involved in controlling myogenesis, was formed earlier, and myotubes were seen earlier and more frequently in this group of myoblasts than in the other groups (cells alone without magnetic field, cells containing MPs but without magnetic field, and cells alone with magnetic field). Moreover, we succeeded in differentiation of early muscle cells with striated myofibrils in culture at 0.05 T. The precisely quantitative and stable stimulus induced by a magnetic field developed in the present study offers … More a new approach to entire process of myblast differentiation into myotubes.
We also examined the differentiation of osteoblasts from the viewpoint of their morphology including distribution of actin filaments. After exposure to the magnetic field, the cells containing magnetic microparticles became larger and were elongated along the axis of the magnetic pole. Stress fibers were formed parallel to the axis. Since the stress fibers have been thought to be formed in accordance with the direction of the applied tension, the magnetic field might act as a stimulation necessary for stress fiber formation. Bone nodules and hydroxyapatite crystals were seen more frequently in this group of osteoblasts than in the other groups (cells alone without magnetic field, cells containing magnetic microparticles but without magnetic field, cells alone with magnetic field, cells alone with magnetic field). The differences were statistically significant at p<0.001 by analysis on a day-by-day basis by analysis of variance. The precisely quantitative and stable stimulus induced by a magnetic field developed in the present study offers a new apprpach to elucidate the entire process of osteoblast differentiation. Less

Report

(3 results)
  • 1999 Annual Research Report   Final Research Report Summary
  • 1998 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Yuge L: "Differentiation of myoblasts in accelerated in culture in a magnetic field"In Vitro Cell Dev Biol.. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 弓削 類: "磁場による培養骨芽細胞の分化促進、胞骨格と細胞分化に与える影響"Supace Utllization Research. 16(in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yuge L: "Differentiation is accelerated in culture a magnetics field"Intern Journal of Anatomy and Embryology. 787. 104 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 弓削 類: "Magnetic Microparticleを利用したmechanical tensionよる筋芽細胞の細胞化学的変化"Space Utilization Research. 15. 126-128 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 弓削 類: "Mechanical tension が筋芽細胞の細胞骨格と細胞分化に与える影響"理学療法学. 26(4). 149-150 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] 弓削 類: "Magnetic Microparticleを利用したmechanical tensionによる筋芽細胞及び骨芽・破骨細胞の細胞化学的変化."宇宙科学. 10(4). 146-155 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] YUGE L, KATAOKA K: "Differentiation of myoblasts in accelerated in culture in a magnetic field ."In Vitro Cell Dev Biol. in press,. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] YUGE L, KUMAGAI T, KATAYAMA Y, et al.: "Differentiation is accelerated in culture osteoblasts in a magnetic field ."Space Utilization Research. 16 in press,. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] YUGE L, KATAOKA K, KUMAGAI T, et al.: "Differentiation is accelerated in culture a magnetic field ."Italian Journal of Anatomy and Embryology :. 104. (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] YUGE L, KATAOKA K, KUMAGAI T, et al.: "Differentiation is accelerated in cultured myoblasts in a magnetic field ."Space Utilization Research 15 ,. 15. 126-128 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] YUGE L, KATAOKA K, KUMAGAI T, et al.: "Cytochemical properties of myoblasts subjected to mechanical tension."Physical Therapy Sciences 26,. 26. 149-150 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] YUGE L, KATAOKA K, KUMAGAI T, et al.: "Cytochemical changes in myoblasts or osteoblasts subjected to mechanical tension."Space Sciences 10,. 10. 146-155 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1999 Final Research Report Summary
  • [Publications] Yuge L: "Differentlation of myoblasts in accelerated in culture in a magnetlc fleld."In Vitro Cell Dev Biol.. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 弓削 類: "磁場による培養骨芽細胞の分化促進.胞骨格と細胞分化に与える影響"Space Utilization Research,. (in press)16. (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yuge L: "Differentlation is accelerated in culture a magnetlc fleld."Italian Journal of Anatomy and Embryology. 787. 104-433 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 弓削 類: "Magnetic Microparticleを利用したmechanical tensionによる筋芽細胞の細胞化学的変化"Space Utilization Research,. 15. 126-128 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 弓削 類: "Mechanical tensionが筋芽細胞の細胞骨格と細胞分化に与える影響"理学療法学. 26(4). 149-150 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 弓削 類: "Magnetic Microparticleを利用したmechanical tensionによる筋芽細胞及び骨芽・破骨細胞の細胞化学的変化"宇宙科学. 10(4). 146-155 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 弓削 類: "Magnetic Microparticleを利用したmechanical tensionよる筋芽細胞の細胞化学的変化" 第15回宇宙利用シンポジューム抄録集 日本学術会議宇宙科学研究所編. 126-128 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] 弓削 類: "Mechanical tensionが筋芽細胞の細胞骨格と細胞分化に与える影響" 理学療法学. 25. 51 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yuge L: "Immunocytochemical localization of connexin43 and E-Cadherin in I-407 cells mediated by cAMP." Acta Histochem.Cytochem. 31(5). 427-433 (1998)

    • Related Report
      1998 Annual Research Report

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Published: 1998-04-01   Modified: 2016-04-21  

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