| Project/Area Number |
11138232
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas (A)
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| Allocation Type | Single-year Grants |
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| Research Institution | Osaka University |
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Principal Investigator |
NOMURA Shintaro Osaka University, Associate Professor, 医学系研究科, 助教授 (80159087)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIKAWA Hideki Osaka University, Professor, 医学系研究科, 教授 (60191558)
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| Project Period (FY) |
1999
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1999: ¥5,000,000 (Direct Cost: ¥5,000,000)
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| Keywords | Osteosarcoma / Metastasis / Osteopontin / Bone matrix protein / Transgenic Mice / In situ hybridization / Transcription factor / PEBP2α |
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| Research Abstract |
Histological features of osteogenic tumor are affected of gene expression encoding bone matrix proteins. Therefore, pathological determination of osteogenic tumors, paticularly atipysm, may results of the abnormal gene expression of bone matrix proteins. We examined gene expression patterns encoding bone matrix proteins with surgically-obtained samples and analyzed by northern hybridization and in situ hybridization. Among samples examined, we observed abnormal expression of osteopontin mRNA in the highly metastatic osteosarcoma. These osteosarcomas had different gene expression pattern with normal cells. To investigate the mechanism of upregulated expression of osteopontin gene, the expression of major transcription factors such as PEBP2 alpfa-A and Ets-1 has examined. We detected mRNAs encoding these transcription factors with higher levels in compared to non-metastatic osteosarcoma. In addition, we generated transgenic mice carrying promoter region of osteopontin gene jointed with reoprter gene. some of these trasgenic line showed expression of reporter gene with the manner similar to the normal expression pattern of endogenous osteopontin gene.
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