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Histone acetylation and human leukemia

Research Project

Project/Area Number 11138264
Research Category

Grant-in-Aid for Scientific Research on Priority Areas (A)

Allocation TypeSingle-year Grants
Research InstitutionNATIONAL CANCER CENTER RESEARCH INSTITUTE

Principal Investigator

KITABAYASHI Kazuo  NATIONAL CANCER CENTER RES.INST., SECTION HEAD, 放射線研究部, 室長 (20261175)

Co-Investigator(Kenkyū-buntansha) SHIMIZU Kimiko  NATIONAL CANCER CENTER RES.INST., SENIOR RESEARCHER, 放射線研究部, 主任研究官 (00161414)
MOROHOSHI Fumiko  NATIONAL CANCER CENTER RES.INST., RESEARCHER, 放射線研究部, 研究員 (20157944)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥5,000,000 (Direct Cost: ¥5,000,000)
Fiscal Year 1999: ¥5,000,000 (Direct Cost: ¥5,000,000)
Keywordsleukemia / histone acetylation / cell differentiation / transcription factor / chromosome translocation
Research Abstract

The AML1-CBFβ transcription factor complex is the most frequent target of chromosomal rearrangements in human leukemia. In the t (8 ; 21) translocation associated with acute myeloid leukemia, the AML1 gene is juxtaposed to the MTG8 gene. We show that the resultant AML1-MTG8 gene product specifically and strongly interacts with a 85 kDa phosphoprotein. Molecular cloning of cDNA indicated that the AML1-MTG8-binding proteins (MTGR1 and MTG16) are highly related to MTG8 and similar to the Drosophila Nervy. Comparison of amino acid sequences among MTGR1, MTG8 and Nervy revealed four evolutionarily conserved regions (NHR1-NHR4). Ectopic expression of AML1-MTG8 in L-G murine myeloid progenitor cells inhibits differentiation to mature neutrophils and induces cell proliferation in response to G-CSF.Analysis with C-terminal deletion mutants of AML1-MTG8 indicated that the region of 51 residues (488 to 538), which contains NHR2, is essential for the induction of G-CSF-dependent cell proliferation … More . Immunoprecipitation analysis indicates that this region is required for AML1-MTG8 to form a stable complex with MTGR1. Overexpression of MTGR1 stimulates AML1-MTG8 to induce G-CSF-dependent proliferation of L-G cells and to interfere with AML1-dependent transcription. These results suggest that AML1-MTG8 could function as a complex with MTGR1, and that the complex might be important in promoting leukemogenesis. The normal AML1 proteins reverses the inhibition of cell differentiation by AML1-MTG8 and restores the competence to differentiate. Immunoprecipitation analysis shows that p300 and CBP interact with AML1. The C-terminal region of AML1 is responsible for the induction of cell differentiation and for the interaction with p300. Overexpression of p300 stimulates AML1-dependent transcription and the induction of cell differentiation. These results suggest that p300 plays critical roles in AML1-dependent transcription during the differentiation of myeloid cells. We also found that the MOZ gene is fused to the p300 gene in a t(8 ; 22) translocation associated with acute myeloid leukemia, resulted in expression of a MOZ-p300 fusion acetyltransferase. Less

Report

(2 results)
  • 2000 Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (29 results)

All Other

All Publications (29 results)

  • [Publications] H.shimada et al.: "Analysis of genes under the downstream control of the t(8 : 21) fusion protein AML1-MTG8 : overexpression of the TISb (ERF-1, cMG1) gene induces myeloid cell proliferation in response to G-CSF."Blood. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Kikuchi et al.: "AML1-MTG8 leukemic protein induces the expression of Granulocyte Colony-stimulating factor (G-CSF) receptor through the upregulation of CCAAT/enhancer binding protein epsilon."Blood. (in press). (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] F.Morohoshi et al.: "Structure and expression pattern of a human MTG8/ETO family gene, MTGR1."Gene. 241. 287-295 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Narita et al.: "Consistent detection of CALM-AF10 chimeric transcripts in haematological malignancies with t(10 : 11)(p13 : q14) and identification of novel transcripts."British J.Haematol. 105. 928-937 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Ichikawa et al.: "Dual transforming activities of the FUS (TLS)-ERG leukemia tusion protein conterred by two N-terminal domains of FUS (TLS)."M.C.B.. 19. 7639-7650 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Tanaka et al.: "Detection of translocation 8 : 21 on interphase cells from acute myelocytic leukemia by fluorescence in situ hybridization and its clinical application."Caner Genet.Cytogenet. 113 : 29-35,1999.. 113. 29-35 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] N.Takahashi et al.: "Base substitution spectra of nalidixylate resistant mutations indduced by monochromatic soft X and ^<60>Co γ-rays in Bacillus subtilis spores."J.Radiat.Res.. 40. 115-124 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] I.Kitabayashi et al.: "Interaction and functional cooperataion of the leukemia-associated factors AML1 and p300 in myeloid cell differentiation. EMBO J.17, 2994-3004, 1998."EMBO.J.. 17. 2994-3004 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] I.Kitabayashi et al.: "The AML1-MTG 8 leukemic fusion protein torms a complex with a novel member of the MTG8 (ETO/CDR) family, MTGR1."Mol.Cell.Biol.. 18. 846-858 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] F.Morohoshi et al.: "Genomic structure of the human RBP56/HTAF1168 and FUS/TLS genes."Gene. 221. 191-198 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Gamou et al.: "The partner gene of AML1 in t(1621) myeloid malignancies is a novel member of the MTG8 (ETO) family."Blood 91, 4028-4037, 1998. 91. 4028-4037 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Shimada, H.Ichikawa, S.Nakamura, R.Katsu, M.Iwasa, I.Kitabayashi and M.Ohki: "Analysis of genes under the downstream control of the t (8 ; 21) fusion protein AML1-MTG8 : overexpression of the TISb(ERF-1, cMG1) gene induces myeloid cell proliferation in response to G-CSF."Blood. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Shimizu, I.Kitabayashi, H.Ichikawa, N.Kamada, T.Abe, N.Maseki, K.Suzukawa and M.Ohki: "AML1-MTG8 leukemic protein induces the expression of Granulocyte Colony-stimulating factor (G-CSF) receptor through the upregulation of CCAAT/enhancer binding protein epsilon."Blood. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] F.Morohoshi, S.Mitani, N.Mitsuhashi, I.Kitabayashi, E.Takahashi, M.Suzuki, Munakata and M.Ohni: "Structure and expression pattern of a human MTG8/ETO family gene, MTGR1."Gene. 241. 287-295 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Narita, K.Shimizu, Y.Hayashi, T.Taki, M.Taniwaki, R.Hosoda, H.Kobayashi, H.Nakamura, N.Sadamori, H.Ohnishi, F.Bessho, M.Yanagisawa, M.Ohki: "Consistent detection of CALM-AF10 chimeric transcripts in haematological malignancies with t (10 ; 11)(p13 ; q14) and identification of novel transcripts."British J.Haematol.. 105. 928-937 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Ichikawa, K.Shimizu, R.Katsu, M.Ohki: "Dual transforming activities of the FUS (TLS) -ERG leukemia fusion protein conferred by two N-terminal domains of FUS (TLS)."Mol.Cell.Biol.. 19. 7639-7650 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] K.Tanaka, M.Arif, H.Asou, K.Shimizu, M.Ohki, T,Kyo, H.Dohy, N.Kamada: "Detection of translocation 8 ; 21 on interphase cells from acute myelocytic leukemia by fluorescence in situ hybridization and its clinical application."Cancer Genet. Cytogenet.. 113. 29-35 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] N.Takahashi, K.Hieda, F.Morohoshi and N.Munakata: "Base substitution spectra of nalidixylate resistant mutations indduced by monochromatic soft X and ^<60>Co γ-rays in Bacillus subtilis spores."J.Radiat.Res.. 40. 115-124 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] I.Kitabayashi, A.Yokoyama, K.Shimizu and M.Ohki: "Interaction and functional cooperataion of the leukemia-asociated factors AML1 and p300 in myeloid cell differentiation."EMBO J.. 17. 2994-3004 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] I.Kitabayashi, K.Ida, F.Morohoshi, A.Yokoyama, N.Mitsuhashi, K.Shimizu, N.Nomura, Y.Hayashi and M.Ohki: "The AML1-MTG 8 leukemic fusion protein forms a complex with a novel member of the MTG8 (ETO/CDR) family, MTGR1."Mol.Cell.Biol.. 18. 846-858 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] F.Morohoshi, Y.Ootsuka, K.Arai, H.Ichikawa, S.Mitani, N.Munakata, M.Ohki: "Genomic structure of the human RBP56/HTAFII68 and FUS/TLS genes."Gene. 221. 191-198 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] T.Gamou, E.Kitamura, F.Hosoda, K.Shimizu, K.Shinohara, Y.Hayashi, T.Nagase, Y.Yokoyama and M.Ohki: "The partner gene of AML1 in t (16 ; 21) myeloid malignancies is a novel member of the MTG8 (ETO) family."Blood. 91. 4028-4037 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] H.Shimada et al.: "Analysis of genes under the downsteam control of the t(8;21) fusion protein AML1-MTG8 : overexpression of the TISb(ERF-1, cMG1) gene induces myeloid cell proliferation in response to G-CSF"Blood. in press (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Shimizu et al.: "AML1-MTG8 leukemic protein induces the expression of Granulocyte Colony-stimulating factor (G-CSF) receptor through the upregulation of CCAAT/enhacer binding protein epsilon"Blood. in press (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] F.Morohoshi et al.: "Structure and expression pattern of a human MTG8/ETO family gene, MTGR1"Gene. 241. 287-295 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] M.Narita et al.: "Consistent detection of CALM-AF10 chimeric transcripts in haematological malignancie with t(10;11)(p13;q14) and identification of novel transcripts"British J. Haematol.. 105. 928-937 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] H.Ichikawa et al.: "Dual transforming activities of the FUS (TLS)-ERG leukemia fusion protein conferred by two N-terminal domains of FUS (TLS)"M. C. B.. 19. 7639-7650 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Tanaka et al.: "Detection of translocation 8;21 on interphase cells from acute myelocytic leukemia by fluorescence in situ hybridization and its clinical application"Cancer Genet. Cytogenet. 113 : 29-35, 1999.. 113. 29-35 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] N.Takahashi et al.: "Base substitution spectra of nalidixylate resistant mutations indduced by monochromatic Soft X and ^<**>Co γ-rays in Bacillus subtilis spores"J. Radiat. Res.. 40. 115-124 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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