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立体構造に基づく蛋白質合成マシーナリーの動作機構の解明

Research Project

Project/Area Number 11169204
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionThe University of Tokyo

Principal Investigator

濡木 理  東京大学, 大学院・理学系研究科, 助教授 (10272460)

Project Period (FY) 1999 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥32,700,000 (Direct Cost: ¥32,700,000)
Fiscal Year 2002: ¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2001: ¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2000: ¥7,200,000 (Direct Cost: ¥7,200,000)
Fiscal Year 1999: ¥20,400,000 (Direct Cost: ¥20,400,000)
Keywords遺伝情報変換 / タンパク質合成 / アミノアシルtRNA合成酵素 / トランスファーRNA / RNAの転写後修飾 / DNA複製 / X線結晶構造解析 / 蛋白質合成 / RNA転写後修飾 / RNAスプライシング
Research Abstract

本研究の目的は、遺伝情報の変換過程(複製、転写、翻訳)に携わる酵素およびRNAの高次構造を決定し、生物マシーナリーとしての動作機構を解明することにある。本年は、まず超好熱古細菌Methanococcus jannashii由来のチロシルtRNA合成酵素(TyrRS)とtRNA^<Tyr>とL-チロシンの複合体の結晶構造を2.0解能で決定し、真核型TyrRSによるtRNAの認識機構を解明し、さらにアンバーサプレッサーtRNA^<Tyr>を効率よくチャージするTyrRS変異体をデザイン、調製することに成功した(論文投稿中)。本古細菌のTyrRSは、大腸菌のtRNA^<Tyr>とクロスリアクトすることがないため、本TyrRSとアンバーサプレッサーtRNA^<Tyr>を大腸菌内で発現させることによって、アンバー終止コドンに21番目の非天然型アミノ酸を取り込ませることが本研究により可能になった。さらに、大腸菌由来TyrRSに2つの変異を入れることによって3-ヨードチロシンをtRNA^<Tyr>にチャージする変異体TyrRSを作り出すことに成功し、この変異体TyrRSと3-ヨードチロシンとの複合体の結晶構造解析を行い、変異体TyrRSが非天然型アミノ酸を認識する構造的基盤を明らかにした。tRNAの3ユ末端のトリミングを行うRNase PHの結晶構造を2.7解能で決定し、3ユ末端のCCA配列直前まで正確に削りこむ機構を推定した(論文改訂中)。また、古細菌由来のクラスICCA付加酵素の結晶構造を2.0解能で決定し、CCA付加酵素の分子進化機構を解明した(論文準備中)。tRNAの修飾過程に関しては、古細菌のtRNAのG15にアーケオシンを導入するアーケオシングアニントランスグリコシラーゼとtRNAの複合体の結晶構造を決定し、酵素がtRNAを大きく変形して、配列非特異的かつ位置特異的に、G15に修飾を入れる機構を解明した(論文改訂中)。さらに、tRNAのG18をメチル化するspoUに関して、酵素とメチル基供与体であるSAMとの複合体の結晶構造を1.5解能で決定し、触媒ドメインに存在する特徴的な結び目構造の持つ役割を解明した(論文準備中)。ヘミメチル化DNAに結合することで、細胞分裂に伴うDNA複製の回数を調節するSeqAタンパク質とDNAの複合体を2.5解能で決定し、ヘミメチル化DNAの認識機構を明らかにした(論文投稿中)。また、DNAの複製開始に関わるDnaAタンパク質とDNAの複合体の結晶構造を2.0&ェ解能で決定し、DNA認識機構を解明した(Nucleic Acid Res., in press)。さらに、ヒト由来EGFとEGFレセプターの複合体の結晶構造を解明し、リガンドによる新規のレセプター活性化機構を明らかにした(Cell,2002)。

Report

(4 results)
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] T.L.Hendrickson, T.K.Nomanbhoy, V.Crecy-Lagard, S.Fukai, O.Nureki et al.: "Mutation Separation of two pathways for editing by a class I tRNA synthetase"Molecular Cell. 9. 353-362 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] R.Ishitani, O.Nureki et al.: "Crystal structure of archaeosine tRNA-guanine transglycosylase"J.Mcl.Biol. 318. 665-667 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] O.Nureki et al.: "An enzyme with a deep trefoil knot for the active-site architecture"Acta Crystallographica D. 58. 1129-1137 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] W.Kagawa, H.Kurumizaka, S.Fukai, R.Ishitani, O.Nureki et al.: "Crystal structure of the human Rad52 protein and its implications for homologous recombination"Molecular Cell. 10. 359-371 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Ogiso, R.Ishitani, O.Nureki et al.: "Crystal structure of human epidermal growth factor in complex with the extracellular domains of the receptor"Cell. 110. 775-787 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] S.Sekine, O.Nureki et al.: "Two alternative ATP-binding modes mediate tRNA-dependent activation of Glu-tRNA synthetase"EMBO J.. 23. 1-13 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Sekine, S., O.Nureki et al.: "Structural basis for anticodon recognition by discriminating glutamyl-tRNA synthetase"Nature Struct. Biol.. 8. 203-206 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kurimoto, K., Fukai, S., O.Nurekiet al.: "Crystal structure of human AUH protein, a single-stranded RNA-binding homologue of enoyl-CoA hydratase"Structure. 9. 1253-1263 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shimada, A., O.Nureki et al.: "Structural and mutational studies of the recognition of the argine tRNA-specific major identity determinant, A20, by arginyl-tRNA synthetase"Proc. Natl. Acad. Sci. USA. 98. 13537-13542 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Nakama, T., O.Nureki et al.: "Structural basis for the recognition of isoleucyl-adenylate and an antibiotic, mupirocin, by isoleucyl-tRNA synthetase"J. Biol. Chem.. 276. 47387-47393 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] TanaKa, Y.^*, O.Nureki^* et al.(^*equal contribution): "DNA kink at the contact site with an arginine-rich helix of human centromere protein B"EMBO J.. 20. 6612-6618 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Terada, T.^*, O.Nureki^* et al.(^*equal contribution): "Functional convergence of two lysyl-tRNA synthetases with unrelated topologies"Nature Struct. Biol.. 9(印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 濡木 理: "蛋白質核酸酵素"アミノアシルtRNA合成酵素aaRSのtRNA認識機構. 46, 1651-1660 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kukimoto,M.,O.Nureki et al.: "Crystallization and preliminary X-ray diffraction analysis of the extracellular domain of the cell surface antigen CD38 complexed with ganglioside"J.Biochemistry. 127. 181-184 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fukai,S.,O.Nureki et al.: "Structural basis for double-sieve discrimination of the cognate L-valine from L-isoleucine and L-threonine by the complex of tRNA^<Val> and valyl-tRNA"Cell. 103. 793-803 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shimada,A.,O.Nureki,N.Dohmae,K.Takio,and S.Yokoyama: "Gene cloning, expression, crystallization, and preliminary X-ray analysis of Thermus thermophilus arginyl-tRNA synthetase"Acta Crystallographica D. 57. 272-275 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sekine,S.,O.Nureki,A.Shimada,D.G.Vassylyev and S.Yokoyama: "Structural basis for anticodon recognition by discriminating glutamyl-tRNA synthetase"Nature Structural Biol.. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Sekine,S.,A.Shimada,O.Nureki. et al.: "Crucial role of the HIGH-loop lysine for the catalytic activity of arginyl-tRNA synthetase"J.Biol.Chem.. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] Handa, N., O. Nureki et al.: "Structural basis for recognition of the tra mRNA precursor by the Sex-lethal protein"Nature. 398. 579-585 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sekine, S., O. Nureki., M. Tateno, and S. Yokoyama: "The identity determinants required for the discrimination between tRNA (Glue) and tRNA (Asp) by glutamyl-tRNA synthetase from Escherichia coli"Eur. J. Biochem.. 261. 354-360 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Nureki, O. et al.: "Proofreading by isoleucyl-transfer RNA synthetase"Science. 283. 459a (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Mechulam, Y., Schmitt, L. Maveyraud, C. Zelwer, O, Nuerki, et al.: "Crystal structure of Escherichia coli methionyl-tRNA synthetase highlights species-specific features"J. Mol. Biol.. 294. 1287-1297 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sugiura, I., O. Nureki, et al.: "The 2.0A crystal structure of Thermus thermophilus methionyl-tRNA synthetase reveals two RNA-binding modules"Structure. 8. 197-208 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kukimoto, M., O. Nureki et al.: "Crystallization and preliminary X-ray diffraction analysis of the extracellular domain of the cell surface antigen CD38 complexed with ganglioside"J. Biochemistry. 27. 181-184 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 濡木理: "化学と工業"tRNAへのアミノ酸の結合-その起源と進化-. 50,181-189 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2018-03-28  

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