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Isolation of Plastics-Degrading Thermophiles and Degradation of Plastics by Them

Research Project

Project/Area Number 11217215
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionKanto Gakuin Universtiy

Principal Investigator

TOMITA Kosuke  Kanto Gakuin University, College of Engineering, Professor, 工学部, 教授 (80237114)

Project Period (FY) 1999 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥13,800,000 (Direct Cost: ¥13,800,000)
Fiscal Year 2002: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2001: ¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2000: ¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 1999: ¥4,300,000 (Direct Cost: ¥4,300,000)
KeywordsPlastics Degrading Microbe / Thermophile / Poly (L-Lactic Acid) / Poly (D-Lactic Acid) / Poly (Ethylene-co-Vinyl Alcohol) / Nylon / Poly (Butylene Succinate-co-Butylene Adipate) / エチレンビニルアルコール共重合体 / Geobacillus sp. / ポリ(L-乳酸)共重合体 / ナイロン12 / ナイロン6 / 脂肪族ポリエステル / Bacillus sp. / ポリ(エチレン-co-ビニルアルコール) / Bacillus stearothermophilus / ポリ(ブチレンスクシナート / ブチレンアジパート)共重合体
Research Abstract

Thermophiles degrading several kinds of plastics, mainly aliphatic polyesters, were successfully isolated from soils. The results are summarized by plastics as follows.
Aliphatic Polyesters : At first, a thermophile degrading poly (L-lactic acid) (PLLA) was isolated. It was not only the first thermopile degrading PLLA, but also the first microbe degrading PLLA except for Actinomycete. This strain was assumed to degrade PLLA endogenously and exogenously. As for the latter degradation, some enzymatic informations were obtained. Afterwards, another strain was isolated. Both of them were of Bacillaceae, but belonged to rather different line from each other, which suggests that PLA-degrading strains do not center on a particular genus. Moreover, a thermophile degrading poly (butylene succinate-co-butylene adipate) (PBSA) was isolated. It was not only the first thermophile degrading PBSA, but also the second microbe degrading PBSA.
New Materials Based on Poly (Lactic Acid) : Many novel informations on the biodegradation of copolyesters of PLLA, poly (D-lactic acid) (PDLA), etc. were obtained through collaboration with "Chemical Synthesized Polyester Group" in this area. Especially, PDLA regarded as a nonbiodegradable material was found to be markedly biodegraded by the strain degrading PBSA, which is the first information on the microbial degradation of PDLA in the world.
Others : Thermophiles degrading poly (ethylene-co-vinyl alcohol) and nylon taken up as references for molecular design of biodegradable material were isolated for the first time in the world, respectively. As for the latter, two strains were obtained. One degraded nylon 12 and nylon 6, and another degraded nylon 12 and nylon 66. It is suggestive for nylon 12 to be degraded by both strains.

Report

(5 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] K.Tomita, Y.Kuroki, K.Nagai: "Isolation of Thermoph lies Degrading Poly(L-Lactic Acid)"Journal of Bioscience and Bioengineering. 87. 752-755 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Tomita, Y.Kuroki N.Hayashi, Y.Kornukai: "Isolation of a Thermophile Degrading Poly(Butylene Succinate-co-Butylene Adipate)"Journal of Bioscience and Bioengineering. 90. 350-352 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Tomita, N.Hayashi, Y.Kuroki: "Biodegradability of Poly(L-Lactic Acid) by a Thermophile"Proceedings of Fifth International Symposium on Environmental Biotechnology. 1001-1004 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Tomita, Y.Kuroki, T.Nakajima, A.Suzuki: "A Thermophile Degrading Poly(Ethylene-co-Vinyl Alcohol)"Journal of Environmental Biotechnology. 2. 53-58 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Tomita, H.Tsuji, T.Nakajima, et al.: "Degradation of Poly(D-Lactic Acid) by a Thermophile"Polymer Degradation and Stability. 81. 167-171 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 冨田耕右, 辻秀人: "ポリ(D-乳酸)の生分解と応用"ECO INDUSTRY. 8(6). 33-41 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] N.Tomita, N.Hayashi, N.Ikeda, Y.Kikuchi: "Isolation of a Thermophilic Bacterium Degrading Some Nylons"Polymer Degradation and Stability. 81. 511-514 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 冨田耕右(監修および分担): "化学結合から見た有化合の生解性 冨田耕右監修「生分解性ケミカルスとプラスチック」"シーエムシー. 253 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 冨田耕右(監修および分担): "ファナインケミカルスやプラスチックグを指向した生分解性素材の開発動向 冨田耕右監修「生分解性ケミカルスとプラスチック」"シーエムシー. 253 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 冨田耕右(分担): "生分解性プラスチックの微生物分解 井上義夫監修「グリーンプラスチック最新技術」"シーエムシー. 304 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 冨田耕右(分担): "微生物による生分解技術情報協会編「生分解性プラスチックの改質技術と成型加工における課題と対策」"技術情報協会. 356 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Tomita, Y.Kuroki, K.Nagai: "Isolation of Thermophiles Degrading Poly (L-Lactic Acid)"J. Biosci. Bioeng.. Vol.87. 752-755 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Tomita, Y.Kuroki, N.Hayashi, Y.Komukai: "Isolation of a Thermophile Degrading Poly (Butylene Succinate-co-Butylene Adipate)"J. Biosci. Bioeng.. Vol.90. 350-352 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Tomita, N.Hayashi, Y.Kuroki: "Biodegradability of Poly (L-Lactic Acid) by a Thermophile"Proc. Fifth Int. Symp. on Environ. Biotech.. 1001-1004 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Tomita, Y.Kuroki, T.Nakajima, A.Suzuki: "A Thermophile Degrading Poly (Ethylene-co-Vinyl Alcohol)"J. Environ. Biotech.. Vol.2. 53-58 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Tomita, H.Tsuji, T.Nakajima, Y.Kikuchi, K.Ikarashi, N.Ikeda: "Degradation of Poly (D-Lactic Acid) by a Thermophile"Polym. Degrad. Stab.. Vol.81. 167-171 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Tomita, H.Tsuji: "Biodegradation and Application of Poly (D-lactic acid)"ECO INDUSTRY. Vol.8, No.6. 33-41 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Tomita, N.Hayashi, N.Ikeda, Y.Kikuchi: "Isolation of a Thermophilic Bacterium Degrading Some Nylons"Polym. Degrad. Stab.. Vol.81. 511-514 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Tomita: "Chemical Bond Approach to Biodegradability of Organic Compounds"CMC. 10 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Tomita: "Developmental Trend of Biodegradable Material for Fine Chemicals and Plastics"CMC. 17 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Tomita: "Microbial degradation of Biodegradable Plastics"CMC. 191 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Tomita: "Biodegradation by Microbes"Technical Information Institute. 88 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] K.Tomita, Y.Kuroki, T.Nakajima, A.Suzuki: "A Thermophile Degrading Poly (Ethylene-co-Vinyl Alcohol)"Journal of Environmental Biotechnology. 2. 53-58 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Tomita, H.Tsuji, T.Nakajima, Y.Kikuchi, K.Ikarashi, N.Ikeda: "Degradation of Poly (D-Lactic Acid) by a Thermophile"Polymer Degradation and Stability. (in press). (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] 冨田耕右: "生分解性プラスチツクの微生物分解 井上義夫監修「グリーンプラスチック最新技術」"シーエムシー. 304 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] K.Tomita, N.Hayashi, Y.Kuroki: "Biodegradability of Poly(L-Lactic Acid)by a Thermophile"Proceedings of Fifth International Symposium on Environmental Biotechnology. 1001-1004 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Tomita, Y.Kuroki, T.Nakajima, A.Suzuki: "A Thermophile Degrading Poly(Ethylene-co-Vinyl Alcohol)"Journal of Environmental Biotechnology. 2(in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 冨田耕右(相澤益男監修): "耐熱性酵素の新展開(最新酵素利用技術と応用展開)"シーエムシー. 15(370) (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] K.Tomita,Y.Kuroki,N.Hayashi,Y.Komukai: "Isolation of a Thermophile Degrading Poly (Butylene Succinate-co-Butylene Adipate)"Journal of Bioscience and Bioengineering. 90・3. 350-352 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] K.Tomita,N.Hayashi,Y.Kuroki: "Biodegradability of Poly (L-Lactic Acid) by a Thermophile"Proceedings of Fifth International Symposium on Environmental Biotechnology. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] 冨田耕右(監修/共同執筆): "生分解性ケミカルスとプラスチック"株式会社 シーエムシー. 255 (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2018-03-28  

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