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単一細胞機能評価の新手法

Research Project

Project/Area Number 11227202
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Science and Engineering
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

松岡 英明  東京農工大学, 工学部, 教授 (10143653)

Co-Investigator(Kenkyū-buntansha) 橋本 壽正  東京工業大学, 大学院・工学研究科, 教授 (50101001)
夏目 雅裕  東京農工大学, 農学部, 助教授 (10201683)
竹山 春子  東京農工大学, 工学部, 助教授 (60262234)
安藤 譲二  東京大学, 大学院・医学研究科, 教授 (20159528)
本多 裕之  名古屋大学, 大学院・工学研究科, 助教授 (70209328)
Project Period (FY) 1999 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥165,500,000 (Direct Cost: ¥165,500,000)
Fiscal Year 2002: ¥42,200,000 (Direct Cost: ¥42,200,000)
Fiscal Year 2001: ¥43,200,000 (Direct Cost: ¥43,200,000)
Fiscal Year 2000: ¥51,200,000 (Direct Cost: ¥51,200,000)
Fiscal Year 1999: ¥28,900,000 (Direct Cost: ¥28,900,000)
Keywords単一細胞機能 / 電気的シグナル / イネキチナーゼ / 流水せん断応力 / 電気化学的シグナル / 熱シグナル / 遺伝子発現制御 / CA2+ / Ca^<2+> / 単一細胞 / 微小電極 / 電気化学的殺菌 / 形態分化 / 交流熱刺激 / 熱ストレス / 機械受容体
Research Abstract

本研究は、単一細胞に対するストレスシグナルの定量的印加、それに対する細胞応答の計測、そのシグナル・応答の相関解析に基づく単一細胞機能評価法の確立を目的としている。前年度までに、ストレスとして電気的シグナル、電気化学的シグナル、熱シグナル、流れせん断応力シグナル、などを選び、要素技術開発と細胞機能解析を進めてきたが、本年度は最終年度にあたり、単一細胞実験法としてシステムの確立を達成した。具体的には、例えば、イネプロトプラスト単一細胞に対する電気的シグナルの定量的印加(実効膜電位の制御)、細胞応答(カルシウム流入、キチナーゼ遺伝子発現)のリアルタイム測定、単一細胞ナンバリングシステムによって、3-4日に渡る一続きの実験を、細胞毎に記録・相関解析できるようにした。そして「単一細胞操作支援ロボット」(特許出願中)という、世界に例のないユニークな概念の実験システムして結実させた。並行して、新しい電気的シグナル応答遺伝子の発見、電気的遺伝子発現のキー分子と考えられるカルシウムチャンネルのクローニングにも成功した。他のストレスシグナルの例として特記すべきは、熱シグナル(細胞の凍結・融解)に対する細胞応答測定である。独創的な温度波熱分析法で、細胞温度変化を単一細胞単位で、高感度・高速に解析できるシステムを完成した。これにより組織凍結時の温度波イメージングの動的解析が可能となり、例えば細胞凍結時に使用する浸透圧調節剤の性能を、植物組織(タマネギやタバコ)の場合について評価できることを示した。また、単一細胞実験には至らなかったが、酵母に対する電気化学的酸化ストレスでカタラーゼ遺伝子やTCAサイクル関連遺伝子の活性化されること、メラノーマ細胞を移植したマウスに磁気微粒子を投与しメラノーマ細胞内に導入後、高周波磁場照射して熱ストレスを誘導すると腫瘍の消失が促進されること、などの知見も得られた。

Report

(4 results)
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (99 results)

All Other

All Publications (99 results)

  • [Publications] Ki-Bong Oh, Hideaki Matsuoka: "Rapid Viability Assessment of Yeast cells Using Vital Staining with 2-NBDG, a Fluorescent Derivative of Glucose"Intern. J. Food Microbiol.. 76. 47-53 (2002)

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      2002 Annual Research Report
  • [Publications] Hideaki Matsuoka, Yuri Kosai, Mikako Saito, Norihide Takeyama, Hiroshi Suto: "Simultaneous Imaging of Multiple Fluorescent Probes in Bio-Cells"Anal.Sci.. 18. 1321-1324 (2002)

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      2002 Annual Research Report
  • [Publications] Hideaki Matsuoka, Kenji Hashimoto, Shigetoshi Horikiri, Mikako Saito, Kimiko Yamamoto, Joji Ando: "Single-Cell Imaging of Ca^<2+> Influx into Bovine Endotherial Cells Occurring in Response to an Alternating Electric Stimulus"Anal.Sci.. 18. 1205-1208 (2002)

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      2002 Annual Research Report
  • [Publications] Hideaki Matsuoka, Tomoko Kurokawa, Kanenari Oishi, Mikako Saito: "Rapid Detection of Small Number of Viable Cells by Fluorescent Glucose Method"J. Food Safety. 22. 141-153 (2002)

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      2002 Annual Research Report
  • [Publications] Hideaki Matsuoka, Yuri Kosai, Mikako Saito, Norihide Takeyama, Hiroshi Suto: "Single-cell Viability Assessment with a Novel Spectro-imaging System"J.Biotechnol.. 94. 299-308 (2002)

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      2002 Annual Research Report
  • [Publications] Ki-Bong Oh, Yasuo Tajima, Mikako Saito, Il-Moo Chang, Hideaki Matsuoka: "Cytotoxicity of Extracts from Alternaria alternata against Cultured Tobacco BY-2 Cells"Biocontrol Sci. 7(2). 127-130 (2002)

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      2002 Annual Research Report
  • [Publications] Mikako Saito, Hideaki Matsuoka, Kunihiro Kasamo: "Isolation of H^+-translocating ATPase in Tonoplast of Tradescantia virginiana L. Leaf Cells"J.Biotechnol.. 100. 221-229 (2003)

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      2002 Annual Research Report
  • [Publications] Mikako Saito, Shigetoshi Horikiri, Hideaki Matsuoka: "Dielectrophoretic Selection of Viable Single-cells of Rice and Tobacco"Electrochemistry. (in press). (2003)

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      2002 Annual Research Report
  • [Publications] S.Kasai, Y.Hirano, N.Motochi, H.Shiku, M.Nishizawa, T.Matsue: "Simultaneous Detection of Uric Acid and Glucose on a Dual-Enzyme Chip Using Scanning Electrochemical Microscopy/Scanning Chemiluminescence Microscopy"Anal.Chim.Acta. 1-8. 263-270 (2002)

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      2002 Annual Research Report
  • [Publications] M.Nishizawa, K.Takoh, T.Matsue: "Micropatterning of HeLa Cells on Glass Substrates and Evaluation of Respiratory Activity using Microelectrodes"Langmuir. 18. 3645-3649 (2002)

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      2002 Annual Research Report
  • [Publications] M.Nishizawa, A.Takahashi, H.Kaji, T.Matsue: "Micropatterned HeLa Cell Culture on PEG Monolayer-Coated Glass Substrates"Chem.Lett. 9. 904-905 (2002)

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      2002 Annual Research Report
  • [Publications] T.Yasukawa, A.Glidle, J.M.Cooper, T.Matsue: "Electroanalysis of Metabolic Flux from Single Cells in Picolitre-Volume Microsystems"Anal. Chem.. 74. 5001-5008 (2002)

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      2002 Annual Research Report
  • [Publications] Nakagawa H, Wachi M, Woo JT, Kato M, Kasai S, Takahashi F, Lee IS, Nagai K: "Fenton reaction is primarily involved in a mechanism of (-)-epigallocatechin-3-gallate to induce osteoclastic cell death"Biochem Biophys Res Commun. 292. 94-101 (2002)

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      2002 Annual Research Report
  • [Publications] K.Suda, J.-T.Woo, M.Takami, P M Sexton, K.Nagai: "Lipopolysaccharide supports survival and fusion of preosteoclasts independent of TNF-alpha, IL-1 and RANKL"J. Cell Physiol. 190. 101-108 (2002)

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      2002 Annual Research Report
  • [Publications] K.Igarashi, J-T Woo, P H Stern: "The effects of a selective cyclooxygenase-2 inhibitor, celecoxib, on bone resorption and osteoclastogenesis in vitro"Biochemical Pharmacology. 63. 523-532 (2002)

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      2002 Annual Research Report
  • [Publications] Bando, M., Hasegawa, M., Tsuboi, Y., Miyake, Y., Shuna, M., Ito, M., Handa, H., Nagai, K., Kataoka, T.: "The mycotoxin penicillic acid inhibits Fas ligand-induced apoptosis by blocking self-processing of caspase-8 in death-inducing signaling complex"J.Biol.Chem.. 278. 5786-5793 (2003)

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      2002 Annual Research Report
  • [Publications] Ando, I., Tsukumo, Y., Wakabayashi, T., Akashi, S., Miyake, K., Kataoka, T., Nagai, K.: "Safflower polysaccharides activate the transcription factor NF-_κB via Toll-like receptor 4 and induce cytokine production by macrophages"Inter.Immunopharmacol.. 2. 1155-1162 (2002)

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      2002 Annual Research Report
  • [Publications] Kataoka, T., Ito, M., Budd, R.C., Tschopp, J., Nagai, K.: "Expression level of c-FLIP versus Fas determines susceptibility to Fas ligand-induced cell death in murine thymoma EL-4 cells"Cell Res.. 273. 256-264 (2002)

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      2002 Annual Research Report
  • [Publications] Bando, M., Miyake, Y., Shima, M., Wachi, M., Nagai, K., Kataoka, T.: "Actin cytoskeleton is required for early apoptosis signaling induced by anti-Fas antibody but not Fas ligand in murine B lymphoma A20 cells. Biochem."Biophys. Res. Commun.. 290. 268-274 (2002)

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      2002 Annual Research Report
  • [Publications] T Ebihara, H Takayama, Y Yanagida, E Kobatake, Mauo Aizawa: "Thermostabilization of protein A-luciferase fusion protein by single amino acid mutation"Biotech. Lett. 24. 147-149 (2002)

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      2002 Annual Research Report
  • [Publications] Tetsuya Haruyama, Eiry Kobatake, Masuo Aizawa: "Cellular biosensing system for discovery of protein synthesis inhibitor with an electrochemical phosphate modulator to regulate the acid phosphatase gene expression of Saccharomyces cerevisiae"Biosens.Bioelect. 17. 209-215 (2002)

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      2002 Annual Research Report
  • [Publications] H.Funabashi, T.Haruyama, M.Mie, Y.Yanagida, E.Kobatake, M.Aizawa: "Non-destructive monitoring of rpoS promotor activity as stress marker for evaluating cellular physiological status"J.Biotechnol.. 95. 85-93 (2002)

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      2002 Annual Research Report
  • [Publications] H.Funabashi, M.Mie, Y.Yanagida, E.Kobatake, M.Aizawa: "Fluorescent monitoring of cellular physiological status depending on the accumulation of ppGpp"Biotech.Lett.. 24. 269-273 (2002)

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      2002 Annual Research Report
  • [Publications] S.Koyama, M.Horii, Y.Yanagida, E.Kobatake, M.Aizawa: "Pressure sensitivity of HSP7O promoter in transformed 3T3-L1 cells"JAMSTEC J. Deep Sea Res.. 20. 53-58 (2002)

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      2002 Annual Research Report
  • [Publications] T.Yoshino, T.Tanaka, H.Takeyama, T.Matsunaga: "Single Nucleotide Polymorphism In Aldehyde Dehydrogenase 2 Gene Using a Single Nano-Sized Magnetic Particle"Biosens.Bioelectron.. (in press). (2003)

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      2002 Annual Research Report
  • [Publications] H.Ohta, H.Takeyama, H.Nakayama, T.Katoh, T.Matsunaga: "SNP Detection in Transforming Growth Factor-* 1 Gene Using Bacterial Mabnetic Particles"Biosens. Bioelectron.. (in press). (2003)

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      2002 Annual Research Report
  • [Publications] Hashimoto, M., T.Kondo, I.Kozone, H.Kawaide, H.Abe, M.Natsume: "Effects of pamamycin-607 and A-factor on Streptomyces spp"Biosci.Biotech.Biochem.. (in press). (2003)

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      2002 Annual Research Report
  • [Publications] Natsume, M., M.Matsumoto, A.Ohshiro, I.Kozone, M.Hashimoto, H.Abe: "Effect of antibiotics on formation of aerial mycelium and production of phytotoxins in Streptomyces spp"J. Pesticide Sci.. (in press). (2003)

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      2002 Annual Research Report
  • [Publications] T.Hashimoto, J.Morikawa, C.Sawatari: "Relaxation Behavior of ultradrawn poly(ethylene) by temperature wave analysis"J. Thermal Analysis and Calorimetry. vol.70. (2002)

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      2002 Annual Research Report
  • [Publications] R.Satoto, J.Morikawa, T.Hashimoto: "Effect of catalysts on thermally stimulated current in poly(ethylene terephthalate)"Thermal Analysis and Calorimetry. vol.70. (2002)

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  • [Publications] Akikazu Maesono, Youichi Takasaki, Yukio Maeda, Ronald P Tye, Junko Morikawa, Toshimasa Hashimoto: "A new apparatus for thermal diffusivity and specific heat measurements of films and liquids by means of Fourier transform thermal analysis"High Temperatures-High Pressures. volume34. 127-134 (2002)

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      2002 Annual Research Report
  • [Publications] A.Ito, M.Shinkai, H.Honda, K.Yoshikawa, S.Saga, T.Wakabayashi, J.Yoshida, T.Kobayashi: "Heat Shock Protein 70 Expression induces Antitumor Immunity during Intracellular Hyperthermia Using Magnetic Nanoparticles"Cancer Immunology and Immunotherapy. 52(2). 80-88 (2003)

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  • [Publications] M.Suzuki, M.Shinkai, H.Honda, T.Kobayashi: "Anti-cancer effect and immune induction by hyperthermia of malignant melanoma using magnetite particles"Melanoma Research. (in press). (2003)

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      2002 Annual Research Report
  • [Publications] 松岡英明: "新しいスペクトロイメージングシステムによる単一細胞の顕微分光"telecom FRONTIER. No.36. 23-29 (2002)

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  • [Publications] 松岡英明: "植物用遺伝子導入支援ロボットを目指すテクノロジー"農業低温科学研究情報. 9(2). 2-4 (2002)

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  • [Publications] 小谷松大祐, 末永智一: "マイクロ電極を用いたバイオセンシング"マテリアルインテグレーション. 15. 39-43 (2002)

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  • [Publications] 末永智一他, "機器分析実験", 梅沢喜夫, 本水昌二, 渡会 仁, 寺前紀夫編: "顕微・形態分析"東京化学同人. 第8章. (2002)

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  • [Publications] 永井和夫: "緑茶渋み成分EGCGの骨代謝に対する効果治療"85. 165-167 (2003)

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  • [Publications] 森川淳子, 橋本寿正: "フーリエ変換型温度波熱分析法による薄膜の熱物性測定"膜. vol.25. 6316-6317 (2002)

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  • [Publications] 森川淳子, 山本暁, 陳寧媚, 橋本寿正: "温度波熱分析法-液体試料の熱物性測定への応用-"熱測定. 29(1). 27-32 (2002)

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  • [Publications] 井藤彰, 本多裕之, 小林猛: "リポソームを用いた腫瘍への薬剤ターゲティング"Drug Delivery System. 17(4). 347-354 (2002)

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      2002 Annual Research Report
  • [Publications] 斉藤美佳子, 松岡英明: "キャピラリーカーボンマイクロ電極"マテリアルインテグレーション. 15(2). 45-49 (2002)

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      2002 Annual Research Report
  • [Publications] 松岡英明: "「4章.微生物制御法の原理と科学」の中の「4.1.物理的方法」P44-54、「5章.制御における測定法・評価法」P82-95、「8章.菌株保存」p130-136. "微生物制御の科学と工学"(土戸哲明、高麗寛紀、松岡英明、小泉淳一、著)"講談社サイエンティフィク. (2002)

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      2002 Annual Research Report
  • [Publications] 松岡英明, 斉藤美佳子: "マイクロインジェクションを軽労化する単一細胞実験システムの構築"平成14年度マイクロバイオリアクターシステムの構築に関する基礎調査委託事業報告書""社)農林水産技術情報協会(印刷中).

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  • [Publications] Kataoka, T., Nagai, K.: "Molecular dissection of cytotoxic functions mediated by T cells. in "Molecular Anatomy of Cellular Systems" I. Endo et al., (Eds.)"Elsevier Science B. V.. 11 (2002)

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  • [Publications] 大野典也, 相澤益男 監訳: "エヌテイーエス"再生医学-テイッシュエンジニアリングの基礎から最先端技術まで-. (2002)

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  • [Publications] 夏目雅裕: "抗生物質(微生物の生産する生理活性物質)In「次世代の農薬開発-ニューナノテクノロジーによる探索と創製-」;桑野栄一、鈴木義勝、安部浩編"ソフトサイエンス(印刷中). (2003)

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  • [Publications] 松岡英明, 斉藤美佳子: "バイオセンシング・バイオエレクトロニクス"生命工学への招待-基礎と応用-"(松永是編)"朝倉書店. 4 (2002)

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  • [Publications] 松岡英明, 斉藤美佳子: "平成14年度マイクロバイオリアクターシステムの構築に関する基礎調査委託事業報告書"(社)農林水産技術情報協会(印刷中). (2003)

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  • [Publications] K.-B.Oh: "Purification and Characterization of an Autoregulatory substance Capable of regulating the morphological transition in Cnadida albicans"Proc.Natl.Acad.Sci. 98. 4664-4668 (2001)

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  • [Publications] K.-B.Oh: "Detection of antifungal activity in Belamcanda chinensis by a Single-cell Bioassay method and isolation of its compound, Tectorigenin"Biosci.Biotechnol.Bioche.. 65. 939-942 (2001)

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  • [Publications] H.Matsuoka: "Single-cell imaging of Ca2+ influx into bovine endotherial cells occurring in response to an alternating electric stimulus"Proceeding of Joint Symposium on Bio-Sensing and Bio-Imaging. (in press).

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      2001 Annual Research Report
  • [Publications] M.Yokouchi: "Electrochemical killing of Streptococcus mutants causing dental caries"Electrochemistry. (in press).

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      2001 Annual Research Report
  • [Publications] M.Natsume: "Phytotoxin production and werial mycelium formation by Streptomyces scabies and S. acidacabies in vitro"J.General Plant Pathol.. 67(in press). (2001)

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      2001 Annual Research Report
  • [Publications] I.Kozone: "De-N-methylation of pamamycins on aerial mycelium indusing and growth inhibition activities"J.Pesticide Sci.. 26. 149-153 (2001)

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      2001 Annual Research Report
  • [Publications] H.Honda: "Preparation of tumor-specific magnetoliposomes and their application for hyrperrtherima"J.Chem.Eng., Jpn. 34. 66-72 (2001)

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      2001 Annual Research Report
  • [Publications] R.Korenaga: "Sp-1 mediated downregulation of P2X4 receptor gene transcription in endothelial cells exposed to Shear stress"Am.J.Physiol Heart Cric Physiol.. 280. 2214-2221 (2001)

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  • [Publications] S.Kato: "MRNA expression on shape-engineered endothelial cells ; Adhesion molecules ICAM-1 and VCAM-1"J.Biomed Mater Res. 54. 366-372 (2001)

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  • [Publications] H.Zhou: "Imaging localized horse radish peroxidase on glass sureface with scanning electrochemical/chemiluminescence microscopy"Anal.Biochem.. 290. 83-88 (2001)

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      2001 Annual Research Report
  • [Publications] H.Shiku: "Oxygen consumption of single bovine embryos with scanning electrochemical microscopy"Anal Chem.. (in press).

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      2001 Annual Research Report
  • [Publications] S.Ogata: "Dielectrophoretic manipulation of a single cell with dual-microdisk electrode"Bioelectrochemistry. (in press).

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      2001 Annual Research Report
  • [Publications] M.nishiyama: "Substeps within the 8 nm step per ATPase cyclesof single kinesin molecules"Nat.Cell Biol.. (in press).

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  • [Publications] T.Ito: "Determination of absolute concentration of nitroxide radial by radio-frequency EPR imaging"Appl.Magn.Reson.. 20. 257-263 (2001)

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  • [Publications] G.Umitsuki: "Involvement of Rnase G in vivo mRNA metabolism in Escherichia coli"Genes Cells. 6. 403-410 (2001)

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      2001 Annual Research Report
  • [Publications] M.Wachi: "Eacherichia coli ribonuclease G"Methods Enzymol.. 342. 55-63 (2001)

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      2001 Annual Research Report
  • [Publications] K.kobayashi: "Suppression of the hypersensitivity of Escherichia coli acrB mutant to organic solvents by integrational activation of the acrEF operon with the IS1 or IS2 element"J.Bacteriol.. 183. 2646-2653 (2001)

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  • [Publications] R.Ando: "Evaluation ofthe growth inhibition strength of hydrocarbon solvents against Escherichia coli and pseudomonas putida grown in a two-liquid phase culture system consisting of a medium and organic solvent"Extremophiles. 5. 11-15 (2001)

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  • [Publications] A.Ito: "Heat-inducible TNF-a gene therapy combined with hyperthermia using magnetic nanoparticles as a novel tumortargeted therapy"Cancer gene therapy. 8(9). 649-654 (2001)

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  • [Publications] S.Luo: "The dimerization of folded monomers of ribulose 1,5-bisphosphate carboxylase/oxtgenase"J.Biol.Chem. 276. 7023-7026 (2001)

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      2001 Annual Research Report
  • [Publications] K.Nakasone: "Transcriptional regulation under pressure conditions by the RNA polymerase sigma54 factor with a two components regulatory system in Shewanella vilacea"Extremophiles. (in press).

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  • [Publications] H.Matsuoka: "Simultaneous imaging of multiple fluorescent probes in bio-cells"Proceeding of Joint Symposium on Bio-Sensing and Bio-Imaging. (in press).

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      2001 Annual Research Report
  • [Publications] 斉藤美佳子: "キャピラリーカーボンマイクロ電極(Galss capillary carbon microelectrode)"マテリアル インテグレーション. 15(2). 45-49 (2002)

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      2001 Annual Research Report
  • [Publications] 松岡英明: "単一細胞実験技術の開発(Development of single-cell experiment technology)"バイオサイエンスとインダストリー.

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  • [Publications] H.Matsuoka: "Single-cell viability assessment with a novel spectro-imaging"J.Biotechnol. 94(3). 299-308 (2002)

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      2001 Annual Research Report
  • [Publications] 斉藤美佳子: "単一細胞実験法による遺伝子発現解析(Gene expression analysis by single-cell experiment)"生物物理. 41(5). 255-257 (2001)

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  • [Publications] M.Saito: "pH microelectrode-micropipette system for the measurement of enzyme reactions in a picoliter volume of a living plant cell"Anal.Chim.Acta. 404(2). 223-229 (2000)

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  • [Publications] M.Saito: "Elicitor action via cell membrane of a cultured rice cell demonstrated by the single-cell transient assay"J.Biotechnol.. 76(2/3). 227-232 (2000)

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  • [Publications] M.Saito: "Modulation of Ca^<2+> influx into a single rice cell with an electric signal"Electrochemistry. 68(5). 333-336 (2000)

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  • [Publications] M.Saito: "Use of acetylene for the fabrication of a glass capillary carbon microelec-trode"Electrochemistry. 68(11). 924-926 (2000)

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  • [Publications] H.Matsuoka: "Microbioelectronics for single-cell experiment"Electrochemistry. 68(5). 314-320 (2000)

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  • [Publications] 斉藤美佳子: "ボーダーレス電気化学者をめざす"電気化学. 69(2). 128-129 (2001)

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  • [Publications] M.Natsume: "Effect of blue light on endogenous brassinosteroid activity and leaf unrolling in rice seedlings."ITE Letters on Batteroes, New Technologuies & Medicine. 1. 781-785 (2000)

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  • [Publications] M.Natsume: "Teasterone-3-O-b-D-glucopyranoside, a new conjugated brassinosteroid metabolite from lily cell suspension cultures and its identification in lily anthors."Biosci.Biotech.Biochem.. 64. 702-709 (2000)

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  • [Publications] M.Natsume: "Reversible Conversion between Teasterone and Its Ester Conjugates in Lily Cell Culture."J.Pesticide Sci.. 25. 117-122 (2000)

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  • [Publications] M.Natsume: "Effects of De-N-methylation of Pamamycins on Aerial Mycelium-inducing and Growth Inhibition Activities."J.Pesticide Sci.. (in press).

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  • [Publications] H.Honda: "Induction of TNF-a gene expression by heat inducible promoter gadd"Japanese Journal of Hyperthermia Oncology.. 16(2). 91-98 (2000)

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  • [Publications] H.Honda: "Heat induction of reporter gene expression via the gadd 153 promoter and its possible application to hyperthermia treatment of cancer."Cytotechnology.. 33. 131-137 (2000)

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  • [Publications] H.Honda: "Bystander-killing effect and cyclic induction of TNF-a Gene under heat-inducible promoter gadd 153."Journal of Bioscience and Bioengineering.. 90(4). 437-441 (2000)

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  • [Publications] H.Honda: "Preparation of tumor-specific magnetoliposomes and their application for hyperthermia."Journal of Chemical Engineering, Japan.. 34(1). 66-72 (2001)

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  • [Publications] J.Ando: "Sp 1-mediated downregulation of P2X4 receptor gene transcription in endothelial cells exposed to shear stress."Am.J.Physiol Heart Circ.Physiol.. (in press).

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  • [Publications] J.Ando: "mRNA expression on shape-engineered endothelial cells : Adhesion molecules ICAM-1 and VCAM-1."J.Biomed.Mater.Res.. 54. 366-372 (2001)

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  • [Publications] J.Ando: "Fluid shear stress activates Ca^<2+> influx into human endothelial cells via P2X4 purinoceptors."Circ.Res.. 87. 385-391 (2000)

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  • [Publications] J.Ando: "P2X4 receptors mediate ATP-induced calcium influx in human vascular endothelial cells."Am.J.Physiol Heart Circ.Physiol.. 279. 285-292 (2000)

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  • [Publications] H. Matsuoka: "Effects of a pulsing electric signal on the cross membrane potential and the cell division potentiality of a single cell of tobacco"Bioelectrochem. Bioenerg.. 49. 65-72 (1999)

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  • [Publications] H. Matsuoka: "Control of the quantity and the timing of microinjection into biocell with a multifunctional microelectrode"Electrochim. Acta. 44. 3801-3807 (1999)

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  • [Publications] T. Hashimoto: "Thermal diffusivity measurement on composite materials of polymers"Fiber Composites. I (1). 54-57 (1999)

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  • [Publications] M. Shinkai: "Effect of functional magnetic particles on radiofrequency capacitive heating"JAPAN J.cancer Res.. 90. 699-704 (1999)

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  • [Publications] M. Saito: "pH-Microelectrode-micropipette system for the measurement of Enzyme Reaction in Picoliter Space of a Living Plant Cell"Anal. Chim. Acta. 404 (2). 223-229 (2000)

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  • [Publications] K. Yamamoto: "P2x4 receptors mediate ATP-induced calcium influx in human vascular endothelial dells"Am. J. Physiol.. (in press).

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Published: 1999-04-01   Modified: 2021-08-26  

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