| Project/Area Number |
11234204
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | The Institute of Physical and Chemical Research (2002)
Kumamoto University (1999-2001) |
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Principal Investigator |
ABE Kuniya The Institute of Physical and Chemical Research, Technology & Development Team for Mammalian Cellular Dynamics, Team Leader, 動物変異動態解析技術開発チーム, チームリーダー (40240915)
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| Co-Investigator(Kenkyū-buntansha) |
松居 靖久 大阪府立母子保健総合医療センター研究所, 研究部長 (40241575)
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| Project Period (FY) |
1999 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥72,200,000 (Direct Cost: ¥72,200,000)
Fiscal Year 2002: ¥12,800,000 (Direct Cost: ¥12,800,000)
Fiscal Year 2001: ¥12,800,000 (Direct Cost: ¥12,800,000)
Fiscal Year 2000: ¥22,500,000 (Direct Cost: ¥22,500,000)
Fiscal Year 1999: ¥24,100,000 (Direct Cost: ¥24,100,000)
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| Keywords | primordial germ cells / totipotency / development / differentiation / gene expression / Embryonic stem cell / genome / 未分化幹細胞 / 細胞分化 / トランスジェニックマウス / 生殖系列 / 幹細胞 |
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| Research Abstract |
We have established techniques to specifically mark and purify totipotent stem cells as well as primordial germ cells (PGC) from mouse embryos. Using purified PGCs and embryonic cells collected from 16 different developmental stages, representative cDNA libraries have been constructed, and cDNA clones were subjected to one pass sequencing to obtain EST sequences. Homology search of the EST from PGC-expressed genes against GenBank non-redundant database revealed that approximately one third of the cDNA represents novel sequences, and we found a number of known genes whose expression were not known in the PGC in our EST collection. Large-scale RT-PCR expression analysis of about 100 PGC-expressed genes was carried out to examine temporal changes in expression of those genes during germ cell development. We could define distinct gene clusters showing co-expression at specific stages of germ line development. In order to analyze organization of the PGC-expressed genes, 〜2,000 genes have been mapped onto mouse genome. Interestingly, there is a tendency that PGC-expressed genes are clustered on the genome. We have made custom cDNA array using PGC-EST sequences and have done expression analysis, and found that PGC-expressed genes are expressed abundantly in embryonic stem (ES) cells. Taken together, PGC may have expression profile similar to that of ES cell or early embryo like blastocyst implying that these cells share some common regulatory mechanisms for gene expression.
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