• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Molecular Mechanism of Functional Regulation of Spermatiogenic Cells by Interaction with Other Somatic Cells and Eggs

Research Project

Project/Area Number 11235201
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Review Section Biological Sciences
Research InstitutionUniversity of Tsukuba

Principal Investigator

BABA Tadashi  BABA,Tadashi, 応用生物化学系, 教授 (40165056)

Co-Investigator(Kenkyū-buntansha) KASHIWABARA Shin-ichi  KASHIWABARA,Shin-ichi, 応用生物化学系, 助教授 (00254318)
TAKAHASHI Satoru  TAKAHASHI,Satoru, 基礎医学系, 教授 (50271896)
OKAMURA Naomichi  OKAMURA,Naomichi, 基礎医学系, 教授 (30134224)
NISHIMURA Hitoshi  NISHIMURA,Hitoshi, 応用生物化学系, 講師 (80241347)
Project Period (FY) 1999 – 2002
Project Status Completed (Fiscal Year 2003)
Budget Amount *help
¥93,300,000 (Direct Cost: ¥93,300,000)
Fiscal Year 2002: ¥23,400,000 (Direct Cost: ¥23,400,000)
Fiscal Year 2001: ¥20,500,000 (Direct Cost: ¥20,500,000)
Fiscal Year 2000: ¥24,400,000 (Direct Cost: ¥24,400,000)
Fiscal Year 1999: ¥25,000,000 (Direct Cost: ¥25,000,000)
KeywordsMale gamete / Cell-to-cell interaction / Sperm / Sperm maturation / Fertilization / Sertoli cell / Oviduct / Spermatogenesis / 輪卵管
Research Abstract

To elucidate the functional regulation of mouse male gamete by other somatic cells and eggs, we have examined the cell interaction in spermatogenesis, sperm maturation, sperm transit from uterus to oviduct, and fertilization in mouse. The following experimental results have been obtained
(1)cDNA clones encoding a testis-specific poly(A) polymerase, TPAP, a candidate molecule responsible for cytoplasmic polyadenylation of pre-existing mRNAs in spermatogenic cells have been identified from a mouse testis cDNA library. Male mice lacking TPAP resulted in the arrest of spermatogenesis and in the increase of apoptosis in spermatogenic cells. The increased apoptosis before the stage of haploid round spermatids may be due not to direct effects of the spermatogenic arrest, but to a transduction of the spoptotic signals to the earlier stages of spermatogenic cells through Sertoli cells.
(2)In mouse, two different isoforms of ADAM1. ADAM1a and ADAM1b, are produced in the testis. ADAM1a is restricte … More dly localized within the endoplasmic reticulum of testicular germ cells, whereas epididymal sperm contain only ADAM1b on the plasma membrane. The loss of mouse ADAM1a resulted in the male infertility because of the severely impaired ability of sperm to migrate from the uterus into oviduct through the uterotubal junction. However, epididymal sperm of ADAM1a-deficient mice were capable of fertilizing the cumulus-intact, zona pellucida-intact eggs in vitro, despite the delayed dispersal of cumulus cells and the reduced adhesion/binding to the zona pellucida. These results suggest that the ADAM1a/ADAM2 fertilin complex may regulate the selective transport of specific sperm proteins including ADAM3, which are involved in the migration into the oviduct, in the dispersal of cumulus cells, and in the binding to the zona pellucida, from the endoplasmic reticulum of testicular germ cells onto the cell surface.
(3)Male mice lacking sperm PH-20 were still fertile, in spite of a reduced ability to disperse cumulus cells from the cumulus mass. Western blot analysis of sperm extracts revealed the presence of other hyaluronidase(s), except PH-20, presumably within the sperm acrosome. These data provide evidence that PH-20 is not essential for fertilization at least in the mouse, and suggest that the other hyaluronidase(s) may play an important role in the sperm penetration through the cumulus cell layer and/or the egg zona pellucida, possibly in cooperation with PH-20. Less

Report

(5 results)
  • 2003 Final Research Report Summary
  • 2002 Annual Research Report
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (52 results)

All Other

All Publications (52 results)

  • [Publications] Kazuo Yamagata: "Difference of acrosomal serine protease system between mouse and other rodent sperm"Developmental Genetics. 25. 115-122 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ko Ohmura: "A homologue of pancreatic trypsin is localized in the acrosome of mammalian sperm, and is released during acrosome reaction"Journal of Biological Chemistry. 274. 29426-29432 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shin-ichi Kashiwabara: "Identification of a novel isoform of poly(A) polymerase, TPAP, specifically present in the cytoplasm of spermatogenic cells"Developmental Biology. 228. 106-115 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Eri Kodama: "cDNA cloning and functional analysis of ascidian sperm proacrosin"Journal of Biological Chemistry. 276. 24594-24600 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takehide Murata: "The hiiragi gene encodes a poly(A) polymerase, which controls the formation of the wing margin in Drosophila melanogaster"Developmental Biology. 233. 137-147 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hitoshi Nishimura: "The ADAM1a and ADAM1b genes, instead of the ADAM1 (fertilin _) gene, are localized on mouse chromosome 5"Gene. 291. 67-76 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] 柏原 真一: "アクロシンインヒビター"制御タンパク質(インヒビター):廣川書店. (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Arata Honda: "A mouse seine protease TESP5 is selectively included into lipid rafts of sperm membrane presumably as a glycosyiphosphatidylinositol-anchored protein"Journal of Biological Chemistry. 277. 16976-16984 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Daichi Baba: "Mouse sperm lacking cell surface hyaluronidase PH-20 can pass through the layer of cumulus cells and fertilize the egg"Journal of Biological Chemistry. 277. 30310-30314 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yoshinori Takahashi: "Mammalian copper chaperone Cox17p has an essential role in activation of cytochrome c oxidase and embryonic development"Molecular and Cellular Biology. 22. 7614-7621 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shin-ichi Kashiwabara: "Regulation of spermatogenesis by testis-specific, cytoplasmic poly(A) polymerase TPAP"Science. 298. 1999-2002 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Shin Sugiura: "Expression of a testis-specific form of TBP-related factor 2 (TRF2) mRNA during mouse spermatogenesis"Journal of Reproduction and Development. 49. 107-111 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ekyune Kim: "Differential localization of ADAM1a and ADAM1b in the endoplasmic reticulum of testicular germ cells and on the surface of epididymal sperm"Biochemical and Biophysical Research Communications. 304. 313-319 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Tiangang Zhuang: "Transgenic expression of testis-specific poly(A) polymerase TPAP in wild-type and TPAP deficient mice"Journal of Reproduction and Development. (In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hitoshi Nishimura: "Possible implication of fertilin complex between ADAM1a and ADAM2 in the appearance of ADAM3 on sperm surface"Journal of Biological Chemistry. (In press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Yamagata, K.: "Difference of acrosomal serine protease system between mouse and other rodent sperm."Dev. Genet.. 25. 115-122 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Ohmura, K.: "A homologue of pancreatic trypsin localized in the acrosome of mammalian sperm, and is released during acrosome reaction."J. Biol. Chem.. 271. 29426-029432 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kashiwabara, S.: "Identification of a novel isoform poly (A) polymerase, TPAP, specifically present in the cytoplasm of spermatogenic cells."Dev. Biol.. 228. 106-115 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kodama, E.: "cDNA cloning and functional analysis of asciadian sperm proacrosin."J. Biol. Chem.. 276. 24594-24600 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Murata, T.: "The hiiragi gene encodes a poly (A) polymerase, which controls the formation of the wing margin in Drosophila melanogaster"Dev. Biol.. 233. 137-147 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishimura, H.: "The ADAM1a and ADAM1b genes, instead of the ADAM1 (fertilin_) gene, are localized on mouse chromosome 5."Gene. 291. 67-76 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Honda, A.: "A mouse serine protease TESP5 is selectively included into lipid rafts of sperm membrane presumably as a glycosylphosphatidylinositol-anchored protein."J. Biol. Chem.. 277. 16976-16984 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Baba, D.: "Mouse sperm lacking cell surface hyaluronidase PH-20 can pass through the layer of cumulus cells and fertilize the egg."J. Biol. Chem.. 277. 30310-30314 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Takahashi, Y.: "Mammalian copper chaperone Cox17p has an essential role in activation of cytochrome c oxidase and embryonic development."Mol. Cell. Biol.. 22. 7614-7612 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kashiwabara, S.: "Regulation of spermatogenesis by testis-specific, cytoplasmic poly (A) polymerase TPAP."Science. 298. 1999-2002

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Sugiura, S.: "Expression of a testis-specifc form of TBP-related factor2 (TRF2) mRNA during mouse spermatogenesis."J. Reprod. Dev.. 49. 107-111 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kim, E.: "Differential localization of ADAM1a and ADAM1b in the endoplasmic reticulum of testicular germ cells and on the surface of epididymal sperm."Biochem. Biophys. Res. Commun.. 304. 313-319 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Zhuang, T.: "Transgenic expression of testis-specific poly (A) polymerase TPAP in the wild-type and TPAP-deficient mice"J. Reprod. Dev., in press. (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Nishimura, H.: "Possible implication of fertilin complex between ADAM1a And ADAM2 in the appearance of ADAM3 on sperm surface."J. Biol. Chem., in press. (2004)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Kashiwabara S.: "Acrosin Inhibitor"Hirokawa-shoten. 5. (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2003 Final Research Report Summary
  • [Publications] Hitoshi Nishimura: "The ADAM1a and ADAM1b genes, instead of the ADAM1(fertilin α)gene, are localized on mouse choromosome 5"Gene. 291. 67-76 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Arata Honda: "A mouse serine protease TESP5 is selectively included into lipid rafts of sperm membrane presumably as a glycosylphosphatidylinositol-anchored protein"Journal of Biological Chemistry. 277. 16976-16984 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Jindawan Siruntawineti: "Occurrence of small, round vesicles in the acrosome of elongating spermatids from a mouse mutant line with a partial deletion of the Y chromosome."Journal Reproduction and Development. 48. 513-521 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Daichi Baba: "Mouse sperm lacking cell surface hyaluronidase PH-20 can pass through the layer of cumulus cells and fertilize the egg"Journal of Biological Chemistry. 277. 30310-30314 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shin-ichi Kashiwabara: "Regulation of spermatogenesis by testis-specific, cytoplasmic poly(A) polymerase TPAP"Science. 298. 1999-2002 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Shin Sugiura: "Expression of a testis-specific from of TBP-related factor 2(TRF2)mRAN during mouse spermatogenesis"Journal Reproduction and Development. 49. 107-111 (2003)

    • Related Report
      2002 Annual Research Report
  • [Publications] Arata Honda: "A mouse serine protease TESP5 is selectively included into lipid rafts of sperm membrane presumably as a GPI-anchored protein"Journal of Biological Chemistry. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shin-ichi Kashiwabara: "Role of acrosomal matrix proteases in sperm-zona pellucida interactions"Human Reproduction Update. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Eri Kodama: "Spermosin, a trypsin-like protease from ascidian sperm : cDNA cloning, protein structures and functional analysis"European Journal of Biochemistry. 269. 657-663 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hitoshi Nishimura: "The ADAM1a and ADAM1b genes, instead of the ADAM1 (fertilin α) gene, are localized on mouse chromosome 5"Gene. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Eri Kodama: "cDNA cloning and functional analysis of ascidian sperm proacrosin"Journal of Biological Chemistry. 276. 24594-24600 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Takehide Murata: "The hiiragi gene encodes a poly(A) polymerase, which controls the formation of the wing margin in Drosophila melanogaster"Developmental Biology. 233. 137-147 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tsuneatsu Mori: "Molecular and immunological approaches to mammalian fertilization"Journal of Reproductive Immunology. 47. 139-158 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Atsuto Inoue: "The transcript for a novel protein with a zinc finger motif is expressed at specific stages of mouse spermatogenesis"Biochemical and Biophysical Research Communications. 273. 398-403 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shin-ichi Kashiwabara: "Identification of a novel isoform of poly(A) polymerase, TPAP, specifically present in the cytoplasm of spermatogenic cells"Developmental Biology. 228. 106-115 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hitoshi Nishimura: "Analysis of loss of adhesive function in sperm lacking cyritestin and fertilin β"Developmental Biology. (In press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Nakanishi: "Real-time observation of acrosomal dispersal from mouse spperm using GFP as a marker protein"FEBS Lett. 449. 277-283 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Yamagata: "Difference of acrosomal serine protease system between mouse and other rodent sperm"Dev:Genet.. 25. 115-122 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] K.Ohmura: "A homologue of pancreatic trypsin is loculized in the acrosome of mammalian sperm,and is released dming acrosome reaction"J.Biol.Chem.. 274. 29426-29432 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] N.Okamura: "Aporcine homolog of the major secretory protein of human epididy mis,HEI,spenifically binds chakesterol"Biochim.Biophys.Acta. 1438. 377-387 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Y.Z.Jin: "Puritication and properties of major α-D-mannosidase in the lmminal tlaid of porcine epididynis"Biochim.Biophys.Acta. 1432. 382-392 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] S.Nishimura: "A GATA box in the GATA-1 gene hematopoitic enhancer is a critical element in the network of GATA factors"Mol.Cell.Biol.. 20. 713-723 (2000)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2018-03-28  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi