| Project/Area Number |
11236205
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| Research Category |
Grant-in-Aid for Scientific Research on Priority Areas
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NONAKA Masaru The University of Tokyo, Graduate School of Science, Professor, 大学院・理学系研究科, 教授 (40115259)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Hiroshi Shiga University of Medical Science, School of Medicine, Professor, 医学部, 教授 (00110560)
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| Project Period (FY) |
1999 – 2003
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| Project Status |
Completed (Fiscal Year 2003)
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| Budget Amount *help |
¥84,600,000 (Direct Cost: ¥84,600,000)
Fiscal Year 2003: ¥18,900,000 (Direct Cost: ¥18,900,000)
Fiscal Year 2002: ¥18,900,000 (Direct Cost: ¥18,900,000)
Fiscal Year 2001: ¥15,300,000 (Direct Cost: ¥15,300,000)
Fiscal Year 2000: ¥15,300,000 (Direct Cost: ¥15,300,000)
Fiscal Year 1999: ¥16,200,000 (Direct Cost: ¥16,200,000)
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| Keywords | MHC / BAC contig / Polymorphism / Evolution / Inhred Strain / 抗原提示 / 塩基配列決定 / メダカ / BAC / 塩基配列 / ゲノム進化 / 連鎖 |
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| Research Abstract |
Human MHC (major histocompatibility complex) extending over 3.6 Mb at 6p21.3 contains many genes essential for adaptive immunity. The MHC is one of the most polymorphic regions of the human genome, and this polymorphism is believed to be formed by adaptive evolution against various pathogens. To clarify MHC polymorphism in bony fish, we determined the complete nucleotide sequence of the MHC class I region of the HNl strain (Northern Japan population-derived inbred medaka) and compared it with the published sequence of the Hd-rR strain (Southern Japan population-derived inbred medaka) MHC class I region. The compared region (〜 420 kb) was divided into three segments based on degree of sequence conservation. The middle segment (〜lOO kb) was so divergent between HNI and Hd-rR that it is almost impossible to make alignment, whereas the other two segments were easily aligned with an average 96.2% nucleotide identity between HNI and Hd-rR. The middle segment encompassed two MHC class I genes and immunoproteasome subunit genes, PSMB8 and PSMB10, and one class I gene and the PSMB8 gene were extremely divergent between two strains. This result indicates that the middle segment is evolving not only by simple nucleotide substitutions but also by extensive DNA rearrangement such as duplication, deletion and insertion within the medaka species.
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