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Transformation of bio-polymers into dissolved and colloidal organic matters in the ocean

Research Project

Project/Area Number 11304039
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 地球化学
Research InstitutionThe University of Tokyo

Principal Investigator

KOIKE Isao  The University of Tokyo, Ocean Research Institute, Professor, 海洋研究所, 教授 (30107453)

Co-Investigator(Kenkyū-buntansha) SUZUKI Satoru  Center for Marine Environmental Studies, Ehime Univ., Professor, 沿岸環境科学研究センター, 教授 (90196816)
TANOUE Eiichirou  Hydrospheric Atmospheric Research Center, Graduate School of Environmental Studies, Nagoya Univ., Professor, 地球水循環研究センター, 教授 (50133129)
KOGURE Kazuhiro  The University of Tokyo, Ocean Research Institute, Prof., 海洋研究所, 教授 (10161895)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥29,840,000 (Direct Cost: ¥28,700,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2001: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2000: ¥10,600,000 (Direct Cost: ¥10,600,000)
Fiscal Year 1999: ¥14,300,000 (Direct Cost: ¥14,300,000)
KeywordsDissolved organic carbon / Colloidal organic matter / Suspended organic matter / Aggregation process / Refractory organic matter / Thermocline / Microbial activitiy / 溶存蛋白 / 三次元蛍光法 / 躍層 / コロイド粒子 / 溶存有機炭素 / 溶存有機窒素 / 珪藻 / 原子力間顕微鏡 / 微細鞭毛虫類 / C / N比 / 細胞外酸性多糖類 / 難分解性有機物 / 生体高分子 / 群集 / 植物プランクトン群集
Research Abstract

In the ocean, biochemical characters of bio-polymers produced by various planktonic assemblages are modified through the interaction with microbes and abiotic processes such as UV-radiation, and are believed to obtain their refractory characters. At the same time, aggregation of modified bio-polymeis into suspended or settling particle size would occur. In this project, fate of bio-polymers in the upper layer of the ocean was chased through extensive field process studies together with simple model experiments in the laboratory. One of the focus of the project is to determine biological and physico-chemical factors controlling aggregation of colloidal and submicron organic particles into large aggregates having enough settling velocities. Following findings and interpretation were obtained from this project. 1) Determination of submicron sized TEP concentration in environmental gradient from coastal waters to oligotrophic open waters prevailed that, irrespective of the depth and areas, … More some 70-80% of total TEP concentrations were located in the submicron-sized fraction, suggesting importance of colloidal sized TEP to form TEP of suspended dimension. 2) Production of semi-refractory organic carbon and nitrogen through microbial uptake and subsequent metabolisms of simple organic matters such as amino acids and glucose was observed within 10-15 days. There is striking similarities of biochemical characters between those freshly formed semi-refractory organic matters and those obtained in open ocean samples, suggesting that microbial processes are a possible factor for the formation of semi-refractory organic matters in the ocean.. 3) Significant contribution of attached micro-flagellates on the aggregation of submicron particles into large aggregates in the upper ocean. The above possibility was tested by an experimental approach using artificial submicron particles, and we obtained significant stimulation of aggregation processes by feeding current of attached microflagellates at least in coastal marine environments. 4) Detection of 100-150 micron sized aggregates within thermocline of coastal waters, sensed by in situ particle size counter. Using a newly developed sensor, clear difference of size distribution of suspended particles between in the mixing layer and within theirmocline was observed, which is direct evidence of active aggregation processes in the upper water column. Less

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Yamada, N., E.Tanoue: "Detection and partial characterization of dissolved glycoproteins in oceanic waters"Mar. Chem.. (submitted). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamashita, Y., E.Tanoue: "Chemical characterization of protein-like fluorophores in DOM in relation to aromatic amino acids"Mar. Chem.. (submitted). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ogawa, H., E.Tanoue: "Dissolved organic matter in oceanic seawater"J. Oceanogr.. (submitted). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tanoue, E.: "Dissolved organic matter, its chemical image, source and enigma"Bull. Coastal Oceanogr. (in Japanese with English abstract). 40(in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Obayashi, Y., S.Hara, K.Suzuki, E.Tanoue: "Characterization of phytoplankton communities in and around the Kuroshio Warm-Core Ring 93A in the northwestern North Pacific in autumn 1997 by using chemotaxonomic pigment analysis"J. Plankton Res.. (submitted). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Obayashi, Y., E.Tanoue: "Growth and mortality rates of phytoplankton in the northwestern North Pacific estimated by dilution method with HPLC pigment analysis"J. Exp. Mar. Biol. Ecol.. (submitted). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamada, N. and E. Tanoue: "Detection and partial characterization of dissolved glycoproteins in oceanic waters."Mar. Chem.. (submitted). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamashita, Y. and E. Tanoue: "Chemical charactetization of protein-like fluorophores in DOM in relation to aromatic amino acids"Mar. Chem.. (submitted). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ogawa, H. and E. Tanoue: "Dissolved organic matter in oceanic seawater"J. Oceanogr.. (submitted). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tanoue, E.: "Dissolved organic matter, its chemical image, source and enigma (in Japanese with English abstract)"Bull. Coastal Oceanogr.. 40(in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Obayashi, Y., S. Hara, K. Suzuki and E. Tanoue: "Characterization of phytoplankton communities in and around the Kuroshio Warm-Core Ring 93A in die north-western North Pacific in autumn 1997 by using chemotaxonomic pigment analysis"J. Plankton Res.. (submitted). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Obayashi, Y. and E. Tanoue: "Growth and mortality rates of phytoplankton in the northwestern North Pacific estimated by dilution method with HPLC pigment analysis"J. Exp. Mar. Biol. Ecol.. (submitted). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Volkman, J. and E. Tanoue: "Chemical and biological studies of paniculate organic matter in the ocean"J. Oceanogr.. 265-279 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hamanaka, J., E. Tanoue, T. Hama and N. Handa: "Production and export of particulate fatty acdis, carbohydrates and combined amino acids in the euphotic zone"Mar. Chem.. 77. 55-69 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wu F. and E. Tanoue: "Tryptophan in sediments of lakes from Southwestern China Plateau"Chemical Geology. (accepted). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wu F. and E. Tanoue: "Sensitive determination of dissolved tryptophan in freshwater by alkaline hydrolysis and HPLC"Analytical Science. 17. 1063-1066 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 田上英一郎, 小川浩史: "溶存有機物"海の研究. 11. 48-51 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Suzuki, S., Kitamura, S., Chiura, H. X.: "Aquabirnavirus widely distrivutes in the ocena,providing a dissolved RNA pool"Microbes and Environments. 16. 191-196 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ymamda, N., Suzuki, S., Tanoue, E.: "Detection of Vivrio angullarum Porin homologue proteins and their source bacteria from coastal seawater"Journal of Oceanography. 56. 583-590 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Volkman, J., Tanoue, E.: "Chemical and biological studies of particulate organic matter In the ocean"Journal of Oceanography. 58. 265-279 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] NAGATA,T.,H.FUKUDA,R.FUKUDA and I.KOIKE :: "Bacterioplankton distribution and production in deep Pacific waters : Large scale geographic variations and possible coupling with sinking particle fluxes."Limnol.Oceanogr.. 45. 426-435 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.HASEGAWA,I.KOIKE,and H.MUKAI: "The fate of food nitrogen in marine copepods."Marine Ecology Progress Series. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.HASEGAWA,I.KOIKE,and H.MUKAI: "Release of dissolved organic nitrogen by planktonic community in Akkeshi Bay."Aquatic Microbial Ecology. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] R. Fukuda,et al.: "East-west gradient in ectoenzyme activities in the subarctic Pacific:"Limnology and Oceanography. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. Hasegawa, I. Koike, H. Mukai: "Release of dissolved organic nitrogen by size-fractionated natural planktonic"Marine Ecology Progress Series. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] H. Kukuda, I. Koike: "feeding current of attached nanoflagellates-a novel mechanism for aggregation"Marine Ecology Progress Series. (in press). (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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