Circadian gene expression of the circadian clock gene cluster kaiABC in cyanobacteria.

• Project/Area Number: 11304055
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 遺伝
• Research Institution: Nagoya University, Center for Gene Research
• Principal Investigator: ISHIURA Masahiro Nagoya University, Center for Gene Research, Professor, 遺伝子実験施設, 教授 (20132730)
• Co-Investigator(Kenkyū-buntansha): KONDO Takao Nagoya University, Graduate School of Science, Professor, 大学院・理学研究科, 教授 (10124223) ; ITOH Shigeru Nagoya University, Graduate School of Science, Professor, 大学院・理学研究科, 教授 (40108634) ; ISHINO Yosizumi Biomolecular Engineering Research Institute, Principal investigator, 主任研究員
• Project Period (FY): 1999 – 2001
• Project Status: Completed (Fiscal Year 2001)
• Budget Amount: ¥37,960,000 (Direct Cost: ¥36,100,000、Indirect Cost: ¥1,860,000); Fiscal Year 2001: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000); Fiscal Year 2000: ¥6,100,000 (Direct Cost: ¥6,100,000); Fiscal Year 1999: ¥23,800,000 (Direct Cost: ¥23,800,000)
• Keywords: Cyanobacteria / Circadian clock / clock gene / kaiABC / Gene expression / Autoregulation / Feedbak regulation / 藍色細菌 / kaiABC / SasA / 分子機構 / クリプトクローム / フォトリアーゼ / 青色光受容体 / 生物発光 / ルシフェラーゼ / リアルタイムモニタリング / シアノバクテリア / 始原菌

Research Abstract

We analyzed the circadian clock in cyanobacteria, focused on the circadian clock gene cluster kaiABC and its gene products, KaiA, KaiB, and KaiC, and obtained the following results:
1 ) KaiC could bind to ATP and GTP, and it was autophosphorylated in the presence of ATP. These activities were necessary to clock oscillation.
2 ) We found SasA which bound to KaiC and enhanced the clock oscillation.
3 ) We analyzed the photolyase/cryptocrome family in cyanabacteria.
4 ) We found two KaiA-binding domains on KaiC.
5 ) We developed a promoter-trap method in cyanobacteria.
6 ) Pex functioned as a negative regulator for the expression of the kaiA gene.

Research Products

• [Publications] Aoki S, Kondo T, Ishiura M: "A promoter-trap vector for clock-controlled genes in the cyanobacterium Synechocystis sp. PCC 6803"J. Microbiol. Methods. (in press).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Taniguchi Y et al.: "Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC"FEBS Letters. 496. 86-90 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Iwasaki et al.: "Physical interactions among circadian clock proteins KaiA, KaiB and KaiC in cyanobacteria"EMBO J.. 18. 1137-1145 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Nishiwaki et al.: "Nucleotide binding and autophosphorylation of the clock protein KaiC as a circadian timing process of cyanobacteria"Proc Natl Acad Sci U S A. 97. 495-499 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Iwasaki et al.: "A KaiC-interacting sensory histidine kinase, SasA, necessary to sustain robust circadian oscillation in cyanobacteria"Cell. 101. 223-233 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Hitomi et al.: "Bacterial cryptochrome and photolyase : characterization of two photolyase-like genes of Synechocystis sp. PCC 6803"Nucleic Acids Res. 28. 2353-2362 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Aoki, S., Kondo, T, Ishiura, M.: "A promoter-trap vector for clock-controlled genes in the cyanobacterium Synechocystis sp. PCC 6803"J. Microbiol. Methods. (in press).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Tanuguchi, Y., et al.: "Two-KaiA-binding domains of cyanobacterial circadian clock protein KaiC"FEBS Lett.. 496. 86-90 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Iwasaki, H., et al.: "Physical interactions among circadian clock proteins KaiA, KaiB and KaiC in cyanobacteria"EMBO. J. 18. 1137-1145 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nishiwaki et al: "Nucleotide binding and autophosphorylation of the clock protein KaiC as a circadian timing process of cyanobacteria"Proc. Natl. Acad. Sci., U. S. A.. 97. 495-499 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Iwasaki, H., et al.: "A KaiC-interacting sensory histidine kinase, SasA, necessary to sustain robust circadian oscillation in cyanobacteria"Cell. 101. 223-233 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Hitomi et al.: "Bacterial cryptochrome and photolyase : characterization of two photolyase-like genes of Synechocysth sp. PCC 6803"Nucleic Acids Res.. 28. 2353-2362 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Aoki S, Kondo T, Ishiura M: "A promoter-trap vector for clock-controlled genes in the cyanobacterium Synechocystis sp. PCC 6803"J. Microbiol. Methods. (in press).
• [Publications] Taniguchi Y et al.: "Two KaiA-binding domains of cyanobacterial circadian clock protein KaiC"FEBS Letters. 496. 86-90 (2001)
• [Publications] Iwasaki et al.: "Physical interactions among circadian clock proteins KaiA, KaiB and KaiC in cyanobacteria"EMBO J.. 18. 1137-1145 (1999)
• [Publications] Nishiwaki et al.: "Nucleotide binding and autophosphorylation of the clock protein KaiC as a circadian timing process of cyanobacteria"Proc Natl Acad Sci U S A. 97. 495-499 (2000)
• [Publications] Iwasaki et al.: "A KaiC-interacting sensory histidine kinase, SasA, necessary to sustain robust circadian oscillation in cyanobacteria"Cell. 101. 223-233 (2000)
• [Publications] Hitomi et al.: "Bacterial cryptochrome and photolyase : characterization of two photolyase-like genes of Synechocystis sp. PCC 6803"Nucleic Acids Res. 28. 2353-2362 (2000)
• [Publications] Nishiwaki,T.,Iwasaki,H.,shiura,M.,and Kondo,T.: "Nucleotide binding and autophosphorylation of the clock protein KaiC as a circadian timing process of cyanobacteria."Proc. Natl. Acad. Sci., U.S.A.. 97:. 495-499 (2000)
• [Publications] Iwasaki,H.,Williams,S.B.,Kitayama,Y.,Ishiura,M.,Golden,S. S.,and Kondo,T.: "A KaiC-interacting sensory histidine kinase, SasA, necessary to sustain robust circadian oscillation in cyanobacteria."Cell. 101:. 223-233 (2000)
• [Publications] Hitomi,K.,Okamoto,K. Daiyasu,H.,Miyashita,H.,Iwai,S.,oh,H.,Ishiura,M.,and Todo,T.: "Bacterial cryptochrome and photolyase : Characterization of two photolyase-like genes of Synechocystis sp.PCC6803."Nucleic Acid.Res.. 28:. 2353-2362 (2000)
• [Publications] Iwasaki,T.: "Physical interactions among circadian clock proteins KaiA,KaiB and KaiC in cyanobacteria."EMBO.J.. 18. 1137-1145 (1999)
• [Publications] Nishiwaki,T.: "Nucleotide binding and autophosphorylation of the clock protein KaiC as a circadian timing process of cyanobacteria"Proc.Natl.Acad.Sci.,U.S.A. 97. 495-499 (2000)