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Research on the plant response to A1 stress and the production of Al tolerant plant

Research Project

Project/Area Number 11306006
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionOKAYAMA UNIVERSITY

Principal Investigator

MATSUMOTO Hideaki  Res. Inst. For Bioresources, Okayama Univ., Professor, 資源生物科学研究所, 教授 (80026418)

Co-Investigator(Kenkyū-buntansha) EZAKI Bunichi  Res. Inst. For Bioresources, Okayama Univ., Assistant Professor, 資源生物科学研究所, 助手 (90243500)
YAMAMOTO Yoko  Res. Inst. For Bioresources, Okayama Univ., Associate Professor, 資源生物科学研究所, 助教授 (50166831)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥32,880,000 (Direct Cost: ¥30,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2001: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2000: ¥11,000,000 (Direct Cost: ¥11,000,000)
Fiscal Year 1999: ¥12,000,000 (Direct Cost: ¥12,000,000)
KeywordsAcid soil / Aluminum stress / Aluminum tolerant gene / Malate exclusion / Transgenic rice / Aluminum injury / Mitochondrial function / Reactive oxygen species / アルミニウム / 毒性 / 耐性獲得 / 植物 / 応答反応 / 微小管 / 耐性 / 脂質過酸化 / 防御機構 / 発現タンパク質
Research Abstract

The major constraint to plant growth in acid soils is the presence of toxic aluminum (Al) cations which inhibit root elongation. The enhanced Al tolerance exhibited by some cultivars of wheat is associated with the Al-dependent efflux of malate from the root apices. Malate forms a stable complex with Al which is harmless to plants, and therefore this efflux of malate forms the basis of a hypothesis to explain Al tolerance in wheat. We carried out the cloning of a wheat gene, ALMT1, that co-segregates with Al tolerance in F_2 and F_3 populations derived from crosses between near-isogenic wheat lines that differ in Al tolerance. The ALMT1 gene encodes a membrane protein that is constitutively expressed in the root apices of the AI-tolerant line at greater levels than in the near-isogenic but Al-sensitive line. Heterologous expression of ALMT1 in Xenopus oocytes, rice and cultured tobacco cells conferred an Al-activated malate efflux.
Potential mechanisms of AI toxicity measured as Al-indu … More ced inhibition of growth in cultured tobacco cells (Nicotiana tabacum, nonchlorophyllic cell line SL) and pea (Pisum sativum) roots were investigated. The accumulation of Al in tobacco cells caused instantaneously the repression of mitochondrial activities and, after a lag of about 12 h, triggered reactive oxygen species (ROS) production, respiration inhibition, ATP depletion, and the loss of growth capability almost simultaneously. The presence of an antioxidant, butylated hydroxyanisol, during Al treatment of SL cells prevented not only ROS production but also ATP depletion and the loss of growth capability, suggesting that the AI-triggered ROS production seems to be a cause of ATP depletion and the loss of growth capability. Furthermore, these three late events were similarly repressed in an Al-tolerant cell line (ALT301) isolated from SL cells, suggesting that the acquisition of antioxidant functions mimicking burylated hydroxyanisol can be a mechanism of Al tolerance. We conclude that A1 affects mitochondrial functions, which leads to ROS production, probably the key critical event in Al inhibition of cell growth. Less

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (37 results)

All Other

All Publications (37 results)

  • [Publications] Matsumoto, H.: "Cell biology of aluminum toxicity and tolerance in higher plants"Int.Rev.Cytol.. 200. 1-46 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sivaguru, M., Fujiwara, T., Samaj, J., Baluska, F., Yang, Z.M., Osawa, H., Maeda, T., Mori, T., Volkmann, D., Matsumoto, H.: "Aluminum-induced 1→3-β-D-glucan inhibits cell-to-cell trafficking of molecules through plasmodesmata : A new mechanism of aluminum toxicity in plants"Plant Physiol.. 124. 991-1005 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yang, Z.M., Sivaguru, M., Horst, W.J., Matsumoto, H.: "Aluminum tolerance is achieved by exudation of citric acid from roots of soybean (Glycine max)"Physiol.Plant.. 110. 72-77 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Devi, S.R., Yamamoto, Y., Matsumoto, H.: "Isolation of aluminum-tolerant cell lines of tobacco in a simple calcium medium and their responses to aluminum"Physiol.Plant.. 112. 397-402 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Osawa, H., Matsumoto, H.: "Possible involvement of protein phosphorylation in aluminum-responsive malate efflux from wheat root apex"Plant Physiol.. 126. 411-420 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamamoto, Y., Kobayashi, Y., Matsumoto, H.: "Lipid peroxidation is an early symptom triggered by aluminum, but not the primary cause of elongation inhibition in pea roots"Plant Physiol.. 125. 199-208 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsumoto, H., Yamamoto, Y., Devi, S.R.: "Metals in the Environment : Analysis by Biodiversity, Prasad, M.N.V.(ed)"Marcel Dekker, Inc.. 31 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsumoto, H: "Cell biology of aluminum toxicity and tolerance in higher plants"Int. Rev. Cytol.. 200. 1-46 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sivaguru, M., Fujiwara, T., Samaj, J., Baluska, F., Yang, Z. M., Osawa, H., Maeda, T., Mori, T.,Volkmann, D. and Matsumoto, H.: "Aluminum-induced 1 → 3-β-D-glucan inhibits cell-to-cell trafficking of molecules through plasmodesmata : A new mechanism of aluminum toxicity in plants."Plant Physiol.. 124. 991-1005 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yang, Z. M., Sivaguru, M., Horst, W. J. and Matsumoto, H: "Aluminium tolerance is achieved by exudation of citric acid from roots of soybean (Glycine max)."Physiol. Plant.. 110. 72-77 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Devi, S. R., Yamamoto, Y. and Matsumoto, H.: "Isolation of aluminum-tolerant cell lines of tobacco in a simple calcium medium and their responses to aluminum."Physiol. Plant.. 112. 397-402 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Osawa, H. and Matsumoto, H.: "Possible involvement of protein phosphorylation in aluminum-responsive malate efflux from wheat root apex."Plant. Physiol.. 126. 411-420 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamamoto, Y., Kobayashi, Y. and Matsumoto, H.: "Lipid peroxidation is an early symptom triggered by aluminum, but not the primary cause of elongation inhibitionin pea roots."Plant.Physiol.. 125. 199-208 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsumoto, H., Yamamoto, Y. and Devi, S. R., M. N. V.(ed).: "Aluminum toxicity in acid soils: Plant response to aluminum. In Metals in the Environment Analysis by Biodiversity"Marcel Dekker ,Inc.. 289-319 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tabuchi, A.: "Changes in cell wall properties of wheat (Triticum aestivum) roots during aluminium-induced growth inhibition"Physiol.Plant.. 112. 353-358 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yamamoto, Y.: "Lipid peroxidation is an early symptom triggered by aluminum, but not the primary cause of elongation inhibition in pea roots"Plant Physiol.. 125. 199-208 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Osawa, H.: "Possible involvement of protein phosphorylation in aluminum-responsive malate efflux from wheat root apex"Plant Physiol.. 126. 411-420 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ahn, S.: "Aluminum inhibits the H^+-ATPase activity by permanently altering the plasma membrane surface potentials in squash roots^1"Plant Physiol.. 126. 1381-1390 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Devi, S.R.: "Isolation of aluminum-tolerant cell lines of tobacco in a simple calcium medium and their responses to aluminum"Physiol.Plant.. 112. 397-402 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ezaki.B: "Different mechanisms of four aluminum (Al) -resistant transgenes for Al toxicity in Arabidopsis^1"Plant Physiol.. 127. 918-927 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 松本 英明: "植物栄養学(森 敏・前忠彦・米山忠克 編)文永堂出版"アルミニウム. 6 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ezaki,B.: "Expression of aluminum-induced genes in transgenic arabidopsis plants can ameliorate aluminum stress and/or oxidative stress."Plant Physiol.. 122. 657-665 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ikegawa,H.: "Responses to aluminium of suspension-cultured tobacco cells in a simple calcium soluton."Soil Sci.Plant Nutr.. 46. 503-514 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Li,X.F.: "Mucilage strongly binds aluminium but does not prevent roots from aluminium injury in Zea mays."Physiol.Plant.. 108. 152-160 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 松本英明: "酸性土壌で発現するアルミニウムストレスに植物はどう応答するか・障害と耐性の分子機構."化学と生物. 38. 452-458 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Matsumoto,H.: "Cell biology of aluminum toxicity and tolerance in higher plants."Int.Rev.Cytol.Academic Press. 200. 1-46 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sivaguru,M.: "Aluminum-induced 1,3-D-glucan inhibits cell-to-cell trafficking of molecules through plasmodesmata : A new mechaninsm of Al Toxicity in plants."Plant Physiol.. 124. 991-1005 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Matsumoto,H.: "Aluminum toxicity in acid soils : Plant response to aluminum."Metals in the Environment : Analysis by Biodiversity, Prasad, M.N.V.(ed) Marcel Dekker, Inc.(未定). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Matsumoto,H: "Plant roots under aluminum stress : Toxicity and tolerance."Plant Roots : The Hidden Half. Third Edition. Waisee, Y., Eshel, A., Kafkafi, U.(eds). Marcel Dekker, Inc.(未定). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Chang,Y-C.: "Effect of Al on the phytosiderophore-mediated solubilization of iron and uptake of iron-phytosiderophore complex in wheat (Triticum aestivum)"Physiol.Plant.. 106. 62-68 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Chang,Y-C.: "Enhancement of callose production by a combination of aluminum and iron in suspension-cultured tobacco (Nicotiana tabacum) cells"Soil Sci.Plant Nutr.. 45. 337-347 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Chang,Y-C.: "Accumulation of aluminium in the cell wall pectin in cultured tobacco (Nicotiana tabacum L.) cells treated with a combination of aluminium and iron"Plant,Cell & Environment. 22. 1009-1017 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Sivaguru,M.: "Differential impacts of aluminium on microtubule organization depends on growth phase in suspension-cultured tobacco cells"Physiol.Plant.. 107. 110-119 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yamaguchi,Y.: "Protective effect of glutathione on the cytotoxicity caused by a combination of aluminum and iron in suspension-cultured tobacco cells"Physiol.Plant.. 105. 417-422 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yamaguchi,Y.: "Cell death process initiated by a combination of aluminium and iron in suspension-cultured tobacco cells (Nicotiana tabacum) : Apotosis-like cell death mediated by calcium and proteinase"Soil Sci.Plant Nutr.. 45. 647-657 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Matsumoto,H.: "Biotechnology and Biodiversity in Agriculture/Forestry(B.N.Prasad ed.) "The mechanisms of Al toxicity and tolerance in higher plants""Oxford and IBH Publishing Co.New Delhi. 18 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Matsumoto,H.: "International Review of Cytology. Kwang W Jeon(ed). "Cell biology of aluminum toxicity and tolerance in higher plants.""Academic Press (in press).

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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