• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study on the induction system of host specificity mutations in Magnaporthe grisea,: avirulence genes and recombinational repair genes

Research Project

Project/Area Number 11306007
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionHokkaido University

Principal Investigator

TOMITA Fusao  Hokkaido University, Graduate School of Agriculture, Professor, 大学院・農学研究科, 教授 (60217536)

Co-Investigator(Kenkyū-buntansha) YASUDA Nobuko  National Agriculture Research Center, Researcher, 中央農業研究センター・北陸研究センター, 研究員 (00355504)
NAKAJIMA Toshihiko  National Agriculture Research Center, Laboratory Head, 中央農業研究センター・北陸研究センター, 室長 (70355585)
ISHII Chizu  Saitama University, Faculty of Science, Associate Professor, 理学部, 助教授 (00114215)
SONE Teruo  Hokkaido University, Graduate School of Agriculture, Assistant Professor, 大学院・農学研究科, 助手 (00333633)
TSUJIMOTO Masako  National Agriculture Research Center, Researcher, 中央農業研究センター・北陸研究センター, 研究員 (80355586)
犬飼 剛  北海道大学, 大学院・農学研究科, 助手 (90223239)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥40,040,000 (Direct Cost: ¥37,700,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2001: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Fiscal Year 2000: ¥7,300,000 (Direct Cost: ¥7,300,000)
Fiscal Year 1999: ¥22,600,000 (Direct Cost: ¥22,600,000)
KeywordsAvirulence genes / Magnaporthe grisea / Recombinational repair / RAD52 / RAD54 / MRE11 / Neurospora crassa / Mutation / Avirulence gene / Neurospora crassa / トランスポゾン / RFLP / Mre11 / Magnaporthe / いもち病 / AFLP / PCR / 変異株 / 組み換え
Research Abstract

(1) Analysis of avirulence genes in M. grisea
Three avirulence genes, Avr-pii, Avr-pia, Avr-Hattan3 was investigated using a cross between two Yunnang isolates. These avirulence genes were mapped onto the genetic map of the fungus. Along the Avr-Hattan 3, a BAC contig of 600kb in length was constructed. On the other hand, Avr-pia was identified also from a cross between Japanese isolate and rare hermaphloditic isolate Guy11. By utilization of the host specificity mutant, a DNA fragment named PM-01, which was tightly linked to the Avr-pia locus was isolated. One of the cosmid clone containing the PM-01 fragment was found to be able to complement the host specificity mutation, indicating that the clone containing the Avr-pia gene.
(2) Analysis of recombnational repair genes in M. grisea
Homologous genes for RAD52, RAD54, and MRE11 was cloned from M. grisea genome, and named Rhm52, Rhm54 and Mhm11, respectively. Among these homologs, Rhm54 was found to complement the phenotype of mus-23 mutant of N. crassa, and to be inducible by MMS treatment in M. grisea cells.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report

Research Products

(14 results)

All Other

All Publications

  • [Publications] T.Sone, S.Fukiya, M.Kodama, F.Tomita: "Molecular structure of rDNA repeat unit in Magnaporthe grisea"Bioscience, Biotechnology & Biochemistry. 64. 1733-1736 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Fukiya, M.Kodama, H.Kito, T.Sone, F.Tomita: "Establishment of a new cross of the rice blast fungus derived from Japanese differential strain Ina168 and hermaphroditic rice pathogen Guy11"Bioscience, Biotechnology & Biochemistry. 65. 1464-1473 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S.Fukiya, T.kuge, T.Tanishina, et al.: "Identification of a putative vacuolar serine protease gene in the rice blast fungus, Magnaporthe grisea"Bioscience, Biotechnology & Biochemistry. 66. 663-666 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H.Kito, Y.Takahashi, J.Sato, S.Fukiya, et al.: "Occan, a novel transposon in the Fot1 Family is ubiquitously found in several Magnaporthe grisea isolates"Current Genetics. 42. 322-331 (2003)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Sone, F.Tomita: "Claral lineages of Japanese Blast-fungus population -variation of pathogenicity & karyotype"Advances in Rice Blast Research. 221-233 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] N.Yasuda, Y.Fujita, M.Tsujimoto: "Identification of avirulence genes of Magnaporthe grisea and selection of Molecular markes for rapid mapping"Advances in the Rice blast fungus. 303-307 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Sone, S. Fukiya, M. Kodama and F. Tomita: "Molecular structure of rDNA repeat unit in Magnaporthe grisea"Bioscience, Biotechnology, and Biochemistry. 64. 1733-1736 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S. Fukiya, M. Kodama, H. Kito, T. Sone and F. Tomita: "Establishment of a new cross of the rice blast fungus derived from Japanese differential strain Ina168 and hermaphroditic rice pathogen Guy11"Bioscience, Biotechnology, and Biochemistry. 65. 1464-1473 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] S. Fukiya, T. Kuge, T. Tanishima, T. Sone, T. Kamakura, I. Yamaguchi and F. Tomita: "Identification of a Putative Vacuolar Serine Protease Gene in the Rice Blast Fungus, Magnaporthe grisea"Bioscience, Biotechnology, and Biochemistry. 66. 663-666 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] H. Kito, Y. Takahashi, J. Sato, S. Fukiya, T. Sone and F. Tomita: "Occan, a novel transposon in the Fot 1 family is ubiquitously found in several Magnaporthe grisea isolates"Current Genetics. 42. 322-331 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Sone and F. Tomita: "Clonal lineages of Japanese blast fungus population - variation of pathogenicity and karyotype"Advances in Rice Blast Research, ed. by D. Tharreau et al.(Kluwer Academic Publishers, Dordrecht, Netherlands). 221-233 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] N. Yasuda, Y. Fujita and M. Tsujimoto: "Identification of avirulence genes of Magnaporthe grisea and selection of molecular markers for rapid mapping"Advances in Rice Blast Research, ed. by D. Tharreau et al.(Kluwer Academic Publishers, Dordrecht, Netherlands). 303-307 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Teruo Sone: "Molecular structure of rDNA repeat unit in Magnaporthe grisea"Bioscience, Biotechnology and Biochemistry. 64(8). 1733-1736 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Teruo Sone: "Advances in Rice Blast Research (ed.by D.Tharreau et al .)"Kluwer Academic Publishers. 364 (2000)

    • Related Report
      2000 Annual Research Report

URL: 

Published: 1999-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi