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Temperature adaptation of fish - molecular mechanisms involved and application

Research Project

Project/Area Number 11306013
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionTHE UNIVERSITY OF TOKYO

Principal Investigator

WATABE Shugo  Graduate School of Agricultural and Life Sciences, The University Of Tokyo, Professor, 大学院・農学生命科学研究科, 教授 (40111489)

Co-Investigator(Kenkyū-buntansha) SAKAI Tadashi  Miyazaki University, Faculty of Agriculture, Professor, 農学部, 教授 (60136794)
USHIO Hideki  Tokyo University of Fisheries, Faculty of Fisheries, Associate Professor, 水産学部, 助教授 (50251682)
KOBAYASHI Makito  Graduate School of Agricultural and Life Sciences, The University Of Tokyo, Professor, 大学院・農学生命科学研究科, 助教授 (30183809)
HIRAYAMA Yasushi  Japan Society for the Promotion of Science, Postdoctoral Researcher, 特別研究員
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥40,470,000 (Direct Cost: ¥38,400,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2001: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2000: ¥10,200,000 (Direct Cost: ¥10,200,000)
Fiscal Year 1999: ¥21,300,000 (Direct Cost: ¥21,300,000)
KeywordsTemperature adaptation / Fish / Mg^<2+>-ATPase activity / Myosin heavy Chain gene / Mitochondria / Membrane voltage potential / Myofiril / 5' upstream region / Mg^<2+>ATPase活性 / 細胞小器官 / チロキシン / 高温耐性ニジマス / 筋小胞体 / ホルモン調節 / 遺伝子調節 / 分子機構 / ミオシン
Research Abstract

Carp acclimates to environmental temperature and induces genes encoding myosin heavy chain isoforms predominantly expressed for fish acclimated to 10 and 30 ℃. Such changes in biochemical changes in carp following temperature acclimation are also observed with intracellular apparatus such as sarcoplasmic reticulum and various enzymes. It seems that, fish alters dynamically many cellular components in response to environmental temperatures. It is considered that environmental temperature signals are transmitted to the central nervous system and subsequently act on gene expression via various hormonal regulations. The objective of this study was to disclose molecular mechanisms involved in temperature adaptation of fish and to utilize the results obtained for industrial application.
The 5' upstream regulatory regions of the 10 ℃- and 30 ℃-type carp myosin heavy chain isoforms were cloned and determined for their structural integrity, resulting in identification of MEF2 binding element res … More ponsible for activation of gene expression. Furthermore, it was found that 5'-upstream regions of myosin heavy chain genes from medaka, another eurythermal fish, also participate in such regulation for temperature-dependent gene expression.
Carp increased expression levels of 55 kDa cytosolic protein in association with acclimation to cold temperature. This protein was found to be one of subunits of mitochondrial FOF1-ATPase and increased its quantities two times following temperature shift from 30 ℃ to 10 ℃. Such different expression depending on acclimation temperature was regulated at the transcription level.
Various fish species were collected from Kasumigaura Lake periodically all around the year and determined for ATPase activity of fast skeletal muscle myofibrils. The activity was the highest with fish collected in November and decreased gradually reaching the minimum value in June and July.
Analytical method to determine mitochondrial membrane voltage was developed by using voltage-sensitive probe and fluorescence imaging. Changes in voltage-associated potential energy of mitochondrial membranes following adaptation to environmental temperatures of fish were successfully measured by this method. Less

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] Yasushi Hirayama et al.: "Structure-function relationships of the two surface loops of myosin heavy chain isoforms From thermally acclimated carp"Biochem. Biophys. Res. Commun.. 269. 24-25 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Makoto Kakinuma et al.: "Differential scanning calorimetry of light meromyosin fragmens having various lengths of carp fast skeletal muscle isoforms"J. Biochem.. 128. 11-20 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Atsushi Kobiyama et al.: "Expression changes of transcripts for fast skeletal myosin heavy chain isoforms during warm temperature acclimation of carp in relation to those of myoD and MEF2 families"Fisheries Sci.. 66. 761-767 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shugo Watabe: "Myogenic regulatory factors and muscle differentiation during ontogeny in fish"J. Fish Biol.. 55(Suppl. A). 1-18 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shigeharu Kinoshita et al.: "cDNA cloning and characterization of the warm-temperature-acclimation-associated protein Wap65 from Carp, Cyprinus carpio"Fish Physiol. Biochem.. 24. 125-134 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shigeharu Kinoshita et al.: "A novel heat stress-responsive gene in the marine diatom Chaetoceros compressum encoding two types of transcripts, a trypsin-like protease and its related protein, by alternative RNA splicing"Eur. J. Biochem. 268. 4599-4609 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shugo Watabe: "Fish Physiology"Ian A.Johnston (Academic Press). 318 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kiyoshi Kikuchi et al.: "Characterization of the carp myosin heavy chain multigene family"Gene. 228. 189-196 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshiaki Nihei et al.: "The mRNA expression patterns of E12 transcription factor in various developmental stages and adult tissues of carp"Fisheries Sci.. 64. 600-605 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kiyoshi Kikuchi et al.: "Increased levels of mitochondrial ATP synthase β-subunit in fast skeletal muscle of carp acclimated to cold temperature"Fisheries Sci.. 64. 629-636 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yasushi Hirayama et al.: "Structure-function relationships of the two surface loops of myosin heavy chain isoforms from thermally acclimated carp"Biochem. Biophys. Res. Commun.. 269. 24-25 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Makoto Kakinuma et al.: "Differential scanning calorimetry of light meromyosin fragments having various lengths of carp fast skeletal muscle isoforms"J. Biochem.. 128. 11-20 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Atsushi Kobiyama et al.: "Expression changes of transcripts for fast skeletal myosin heavy chain isoforms during warm temperature acclimation of carp in relation to those of myoD and MEF2 families"Fisheries Sci.. 66. 761-767 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shugo Watabe: "Myogenic regulatory factors and muscle differentiation during ontogeny in fish"J.Fish Biol.. 55(Suppl. A). 1-18 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shugo Watabe: "Myogenic regulatory factors"Fish Physiology. 18. 19-41 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Md. Nazmul Ahsan et al.: "Molecular cloning and characterization of two isoforms of trypsinogen from anchovy pyloric caeca"Marine Biotechnol.. 3. 80-90 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shiro Itoi et al.: "Analysis on serum proteins from rainbow trout Oncorhynchus mykiss exposed to high temperature"Fisheries Sci.. 67. 191-193 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shigeharu Kinoshita et al.: "cDNA cloning and characterization of the warm-temperature-acclimation-associated protein Wap65 from carp, Cyprinus cardio"Fish Physiol.Biochem.. 24. 125-134 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shigeharu Kinoshita et al.: "A novel heat stress-responsive gene in the marine diatom Chaetoceros compression encoding two types of transcripts, a trypsin-like protease and its related protein, by alternative RNA splicing"Eur.J.Biochem.. 268. 4599-4609 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shugo Watabe: "Expression changes of transcripts for fast skeletal myosin heavy chain isoforms of carp during temperature acclimation"Proceedings of International Workshop on Biology Hanoi, Vietnam, 2- 5 July. 253-256 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Atsushi Kobiyama et al.: "Sprmger- verlag (in press)"Temporal and spatial expression patterns of mRNAs encoding myosin heavy chain isoforms in association with those of related transcription factors during temperature acclimati on of carp "Aquagenome" (Shimizu, N., and Aoki, T., ed).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shigeharu Kinoshita, et al.: "cDNA cloning arid characterization of the warm-temperature-acclimation-associated protein Wap65 from Carp, Cyprinus carpio"Fish Physiol. Biochem.. 24. 125-134 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Md. Nazmul Ahsan, et al.: "Molecular cloning and characterization of two isoforms of trypsinogen from anchovy pyloric caeca"Marine Biotechnol.. 3. 80-90 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hidehiro Kondo, et al.: "The novel structure of major plasma apolipoproteins in the eel Anguilla japonica"Biochim. Biophys. Acta. 1351. 132-142 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Md. Nazmul Ahsan, Shugo Watabe: "Kinetic and structural properties of two isoforms of trypsin isolated from the viscera of Japanese anchovy Engiraulis japonicus"J. Protein Chem.. 20. 49-58 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shigeharu Kinoshita, et al.: "A novel heat stress-responsive gene in the marine diatom Chaetoceros compressum encoding two types of transcripts, a trypsin-like protease and its related protein, by alternative RNA splicing"Eur. J. Biochem. 268. 4599-4609 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shugo Watabe: "Proceedings of International Workshop on Biology (Hanoi, Vietnam, 2-5 July)"Expression changes of transcripts for fast skeletal myosin heavy chain isoforms of carp during temperature acclimation. 253-256 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Atsushi Kobiyama et al.: "Aquagenome (Shimizu, N., and Aoki, T., ed), Springer-Verlag"Temporal and spatial expression patterns of mRNAs encoding myosin heavy chain isoforms in association with those of related transcription factors during temperature acclimation of carp(印刷中). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yasushi Hirayama et al.: "Structure-function relationships of the two surface loops of myosin heavy chain isoforms from thermally acclimated carp"Biochem.Biophys.Res.Commun.. 269. 24-25 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Makoto Kakinuma et al.: "Differential scanning calorimetry of light meromyosin fragmens having various lengths of carp fast skeletal muscle isoforms"J.Biochem.. 128. 11-20 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Chie Yoneda et al.: "Abalone collagens : immunological properties and seasonal changes of their mRNA levels"Comp.Biochem.Physiol.. 126B. 59-68 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Atsushi Kobiyama et al.: "Expression changes of transcripts for fast skeletal myosin heavy chain isoforms during warm temperature acclimation of carp in relation to those of myoD and MEF2 families"Fisheries Sci.. 66. 761-767 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shugo Watabe: "Myogenic regulatory factors and muscle differentiation during onto geny in fish"J.Fish Biol.. 55(Suppl.A). 1-18 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shiro Itoi et al.: "Analysis on serum proteins from rainbow trout Oncorhynchus mykiss exposed to high temperature"Fisheries Sci.. (印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shugo Watabe: "Fish Physiology (Academic Press)"Ian A.Johnston. 318 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kiyoshi Kikuchi et al.: "Characterization of the carp myosin heavy chain multigene family"Gene. 228. 189-196 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yoshiaki Nihei et al.: "The mRNA expression patterns of E12 transcription factor in various developmental stages and adult tissues of carp"Fishries Science. 64. 600-605 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kiyoshi Kikuchi et al.: "Incrensed levels of mitochondrial ATP synthase β-subunit in fast skeletal muscle of carp acclimated to cold temperature"Fishries Science. 65. 629-636 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shugo Watabe: "Myogenic regulatory factors and muscle differentiation during ontogeny in fish"J. Fish Biol.. 55. 1-18 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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