| Project/Area Number |
11307006
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | Kanazawa University |
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Principal Investigator |
OGINO Keiki Kanazawa University, Graduate School of Medical Science, Professor, 医学系研究科, 教授 (70204104)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAJIMA Madoka Kanazawa University, Graduate School of Medical Science, Research Associate, 医学系研究科, 助手 (10324071)
NAGASE Hirofumi Kanazawa University, Graduate School of Medical Science, Assistant Professor, 医学系研究科, 講師 (00251918)
NAKAMURA Hiroyuki Kanazawa University, Graduate School of Medical Science, Associate Professor, 医学系研究科, 助教授 (30231476)
FUJIKURA Yoshihisa Oita Medical University, Professor, 医学科, 教授 (10165368)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥37,190,000 (Direct Cost: ¥34,400,000、Indirect Cost: ¥2,790,000)
Fiscal Year 2001: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
Fiscal Year 2000: ¥14,900,000 (Direct Cost: ¥14,900,000)
Fiscal Year 1999: ¥10,200,000 (Direct Cost: ¥10,200,000)
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| Keywords | eosinophil / peroxidase / nitrotyrosine / immunohistochemistry / NC / Nga mouse / Eol-1 / reactive nitrogen species / heme / アトピー性皮膚炎 / 一酸化窒素(NO) / Ige / 好酸球 / シグナル伝達機構 / 組織固定法 / 二酸化窒素 / ニドロチロシン / パーオキシダーゼ / 小腸 |
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| Research Abstract |
The pathophysiological significance of free-tyrosine or protein-bound tyrosine nitration by the generation of reactive nitrogen species via eosinophil peroxidase was investigated with eosinophils, eosinophil-derived leukemia cells or atopic dermatitis- like-NC/Nga mice. Regarding the immunohistochemical staining method, artifact were occasionally observed in sections containing eosinophils. As a result of this research, an increase in the immunostaining cells for nitrotyrosine in eosinophils of atopic dermatitis-like lesions in NC/Nga mice and the susceptibility of nitrotyrosine staining in eosinophils by reactive nitrogen species derived from adjacent cells were demonstrated. Therefore, it was speculated that eosinophils have a role in the scavenging of reactive nitrogen species. We demonstrated that the expression of myeloperoxidase in human eosinophilic leukemia cell line (Eol-1) occurred when the cells were degenerated by butyric acid and when the ability of nitrotyrosine formation was detected after cytospin preparations of the cells with H_20_2 and NO_2 contrary to the expectation of eosinophil peroxidase expression. Moreover, in further investigations into the localization of peroxidase responsible for the nitration of tyrosine in rat organs, two peroxidases were recognized. One is eosinophil peroxidase found in the gastrointestinal tract and the other is an unknown peroxidase -like that found in the lungs, spleen and heart. In the heart, an unknown peroxidase-like enzymes or the heme protein contributed for the nitration of tyrosine was localized in myocytes.
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