| Project/Area Number |
11307008
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| Research Category |
Grant-in-Aid for Scientific Research (A).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Nagoya University |
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Principal Investigator |
KATSUMATA Yoshinao School of Medicine, Nagoya University, Professor, 医学部, 教授 (30109326)
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| Co-Investigator(Kenkyū-buntansha) |
ITO Kensuke Faculty of Law, Nagoya University, Professor, 法学部, 教授 (00107492)
UCHIHI Rieko School of Medicine, Nagoya University, Research Associate, 医学部, 助手 (20223571)
YAMAMOTO Toshimichi School of Medicine, Nagoya University, Associate Professor, 医学部, 助教授 (50260592)
NUDESHIMA Jiro Mitsubishi Kasei, Institute of Life Sciences, Chief, 社会生命科学研究室, 主任研究員
OBATA Nobuaki Graduate School of Polymathematics, Nagoya University, Associate Professor, 多元数理科学研究科, 助教授 (10169360)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥37,600,000 (Direct Cost: ¥37,600,000)
Fiscal Year 2000: ¥8,400,000 (Direct Cost: ¥8,400,000)
Fiscal Year 1999: ¥29,200,000 (Direct Cost: ¥29,200,000)
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| Keywords | Paternity test / Database / Guideline / STR |
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| Research Abstract |
The first one of results in the present study was "The guideline of paternity test in Japan" fixed up by the working group of paternity test in the Japanese Society of Legal Medicine. The head investigator of this study organized this group as the chairperson to make the guideline in cooperation with the other investigators. This guideline is the first and overall one in Japan concerning the parentage testing, which includes not only technical protocol but also sample collection and storage, the formula of expert report, and ethical guideline. We also recommended the three tests, homozygosity test, likelihood ratio test and exact test for the Hardy-Weiberg equilibrium by re-verification, and constructed a highly reliable database in Japanese at general STR loci available by commercially released multiplex amplification and typing kits. As more practical studies, we applied some specimens such as saliva, plucked hairs and cells from toothbrushes to multiplex STR genotyping thoroughly, and expanded the potential limitation of specimens. Furthermore, we tried to apply the minisatellite variant repeat mapping by PCR (MVR-PCR) to paternity tests. On the other hand, we discussed about the mutation of STRs which has considerably high rates with multiplex STR systems, and calculated the rate using a multiplex system. Addition of this rate in calculating the probabilities makes it possible to construct a more reliable system for parentage testings. In the present study, we have almost established a reliable paternity test system in Japan. It is necessary to make efforts to recognize the courts and society about the rightful paternity tests in future.
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