| Project/Area Number |
11307021
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Gunma University |
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Principal Investigator |
KUWANO Hiroyuki Gunma University, Faculty of Medicine, Professor, 医学部, 教授 (90186560)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMURA Tatsuo Gunma University, Faculty of Medicine, Instructor, 医学部, 助手 (00282393)
KATO Hiroyuki Gunma University, Facutly of Medicine, Assistant Professor, 医学部, 講師 (70224532)
ASAO Takayuki Gunma University, Faculty of Medicine, Associate Professor, 医学部, 助教授 (40212469)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥33,800,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2001: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2000: ¥6,000,000 (Direct Cost: ¥6,000,000)
Fiscal Year 1999: ¥20,000,000 (Direct Cost: ¥20,000,000)
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| Keywords | digestive cancer / mice metastatic model / oncogenesis / co-culture / epigenetic / atypical change / p53 / gap junction / コネキシン / 癌関連遺伝子 / RT-PCR / HLA-DR |
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| Research Abstract |
The purpose of this study was to establish a model of experimental lymph node metastasis by intra-rectal implantation of human cancer cell in nude mice. Four types of human cancer cell lines were investigated. Tumor cells were injected into the submucosal layer of the rectum. All cancer cell lines produced locally aggressive rectal tumors and, subsequently, para-aortic lymph node metastasis. Using this method, we were able to evaluate the effect of anti-cancer agent uracil/tegafur (UFT) on primary tumor growth and lymph node metastasis. Oral intake of UFT significantly suppressed implanted tumor volume and inhibited lymph node metastasis, We expect that the process of lymph node metastasis shown in this model will be studied as an experimental model of lymph node metastasis simulating human cancer.
Malignant Transformation of the Mice Ano-rectal Epithelium Induced by an Inocultated Human Cancer Cell Line
KATOIII, and Lu 135 showed the potential enforcement of atypical changes in the adjacent mice ano-rectal epithelium. Then, each six mice at different times at 1, 2, 4, 6, 8, 10weeks after KATOIII implantation and the groups of five mice each were sacrificed at same periods weeks after Lu 135 implantation. Atypical changes in the mice implanted with KATOIII appeared at 2W (week) and the incidence of an atypical epithelium was 33% at this time. The incidence of an atypical epithelium tended to increase with the passage of time. The total incidence was 47.2% and the incidence after 4W was 62.5%. Moreover, the submucosal invasion of the matignant transformed cells of the mice epithelium was demonstrated in specimens obtained from three KATOIII inoculated mice. This exciting and novel phenomenon clearly demonstrates the need to change the present general concept of single cell origin of cancer tissue and the novel experimental design itself is also extremely useful as a simple model for investigating the mechanisms of oncogenesis.
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