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Development and evaluation of artificial nerve which could enhance axonal elongation in peripheral and central nervous system

Research Project

Project/Area Number 11307037
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Plastic surgery
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

NISHIMURA Yoshihiko  Kyoto Univ Grad. School of Med., Dep. of Plastic surgery Prof., 医学研究科, 教授 (50081790)

Co-Investigator(Kenkyū-buntansha) TANIHARA Masao  Nara Institute of Sci. and Technology., Sci Grad School of material Prof., 物質創成科学研究科, 教授 (50294286)
ENDO Katuaki  Kyoto Univ Grad. School of Med., Dep. of Physioligy Instructor, 医学研究科, 助手 (30025613)
SUZUKI Yoshihisa  Kyoto Univ Grad. School of Med., Dep. of Plastic surgery Assoc. Prof, 医学研究科, 助教授 (30243025)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥36,720,000 (Direct Cost: ¥34,200,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2001: ¥10,920,000 (Direct Cost: ¥8,400,000、Indirect Cost: ¥2,520,000)
Fiscal Year 2000: ¥8,400,000 (Direct Cost: ¥8,400,000)
Fiscal Year 1999: ¥17,400,000 (Direct Cost: ¥17,400,000)
Keywordsneural stem cell / spinal cord / regeneration / central system / injury / immunohistochemistry / sciatic nerve / peripheral nervous system / アルギン酸 / 神経再生 / 脊髄再生 / 末梢神経再生
Research Abstract

Alginate, derived from brown seaweeds was chemically crosslinked with covalent bond. Biodegradation speed can be controlled by initial concentration of alginate and dose of irradiation. For peripheral nerve repair, sciatic nerve gap in the cats was bridged using alginate sponge, which was specially designed to biodegradate within 3 months. Three months after operation, many regenerated axon were found in the middle of the gap. Electrophysiologically, muscle action potentials could be recorded at the gastrocnemius muscle after electrical stimulation of the sciatic nerve proximal to the gap. Rat sciatic nerve gap model, regenerating axons can be seen in the biodegrading alginate, which does not have sponge structure. For central nerve repair, freeze-dried alginate sponge was implanted into the gap of rat spinal cord. Regenerating axons was seen in the implanted alginate and in the host spinal cord, which was examined by HRP-tracing study. Electrophysiologically, action potential was recorded beyond the gap. Next, hippocampus-derived neurospheres were prepared from transgenic rat fetuses expressing green fluorescent protein (GFP), and transplanted into an alginate-filled lesion of young rat spinal cord. One, two and four weeks after transplantation, a large number of grafted cells survived, many of which expressed immunoreactiveity for glial fibrillary acidic protein, and a few expressed immunoreactiveity for beta-tubulin These findings demonstrate that rat fetal hippocampus-derived neurosphere cells could survive, differentiate, and integrate well into the host spinal cord tissue.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (33 results)

All Other

All Publications (33 results)

  • [Publications] Wu, S.F.: "Facial nerve repair using alginate sponge without suturing"Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Suzuki, Y.: "Electrophysiological and HRP-tracing studies of nerve regeneration through alginate-filled gap in adult rat spinal cord"Neuroscience Letters. 318. 121-124 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wu, S.F.: "New method for transplantation of neurosphere cells into injured spinal cord through cerebrospinal fluid in rat"Neuroscience Letters. 318. 81-84 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wu, S.F.: "Migration, integration, and defferentiation of hippocampus-derived neurosphere cells after transplantation into injured rat spinal cord"Neuroscience Letters. 312. 173-176 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wu, S.F.: "Sciatic nerve regeneration through alginate with tubulation or non-tubulation repair in cat"Journal of Neurotrauma. 18. 329-338 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ide C.: "Grafting of choroid plexus ependymal cells promotes the growth of regenerating axons in the dorsal funiculus of rat spinal cord"Experimental Neurology. 167. 242-251 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kataoka, K.: "Alginate, a bioresorbable material derived from brown seaweed, enhances elongation of amputated axons of spinal cord in infant rats"Jounal of Biomedical Materials Research. 54. 373-384 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Suzuki, Y.: "Alginate hydrogel linked with synthetic oligopeptide derived from BMP-2 allows ectopic osteoinduction in vivo"Journal of Biomedical Materials Research. 50. 405-409 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wu, S.F.: "Facial nerve repair using alginate sponge without suturing."Sandinavianc Journal of Plastic and Reconstructive Surgery and Hand Surgery. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Suzuki, Y.: "Electrophysiological and HRP-tracing studies of nerve regeneration through alginate-filled gap in adult rat spinal cord"Neuroscience Letters. 318. 121-124 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wu, S.F.: "New method for transplantation of neurosphere cells into injured spinal cord through cerebrospinal fluid in rat"Neuroscience Letters. 318. 81-84 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wu, S.F.: "Migration, integration, and differentiation of hippocampus-derived neurosphere cells after transplantation into injured rat spinal cord"Neuroscience Letters. 312. 173-176 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wu, S.F.: "Sciatic nerve regeneration through alginate with tubulation or non-tubulation repair in cat"Journal of Neurotrauma. 18. 329-338 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ide C.: "Grafting of choroid plexus ependymal cells promotes the growth of regenerating axons in the dorsal funiculus of rat spinal cord : A preliminary report"Experimental Neurology. 167. 242-251 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kataoka, K.: "Alginate, a bioresorbable material derived from brown seaweed, enhances elongation of amputated axons of spinal cord in infant rats"Journal of Biomedical Materials Research. 54. 373-384 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Suzuki, Y.: "Alginate hydrogel linked with synthetic oligopeptide derived from BMP-2 allows ectopic osteoinduction in vivo"Journal of Biomedical Materials Research. 50. 405-409 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Wu, S.F.: "Facial nerve repair using alginate sponge without suturing"Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery. (in press).

    • Related Report
      2001 Annual Research Report
  • [Publications] Suzuki Y.: "Electrophysiological and HRP-tracing studies of nerve regeneration through alginate-filled gap in adult rat spinal cord"Neuroscience Letters. 318. 121-124 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Wu, S.F.: "New method for transplantation of neurosphere cells into injured spinal cord through cerebrospinal fluid in rat"Neuroscience Letters. 318. 81-84 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Wu, S.F.: "Migration, integration, and differentiation of hippocampus-derived neurosphere cells after transplantation into injured rat spinal cord"Neuroscience Letters. 312. 173-176 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Wu, S.F.: "Sciatic nerve regeneration through alginate with tubulation or non-tubulation repair in cat"Journal of Neurotrauma. 18. 329-338 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ide C.: "Grafting of choroid plexus ependymal cells promotes the growth of regenerating axons in the dorsal funiculus of rat spinal cord"Experimental Neurology. 167. 242-251 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Kataoka K.: "Alginate, a bioresorbable material derived from brown seaweed, enhances elongation of amputated axons of spinal cord in infant rats"Journal of Biomedical Materials Research. 54. 373-384 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sualki Y.: "Alginate hydrogel linked with synthetic oligopeptide derived from BMP-2 allows ectopic osteoinduction in vivo"Journal of Biomedical Materials Research. 50. 405-409 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Suzuki K: "Regeneration of transected spinal cord in young adult rats using freeze-dried alginate gal."Neuroreport. 10. 2891-2894 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Suzuki K: "Reconstruction of rat peripheral nerve gap without sutures using freeze-dried alginate,"Journal of Biomedical Materials Research. 49. 582-533 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kataoka K: "Alginate, a bioresorbable material derived from brown seaweed, enhances elongation of amputated axons of spinal cord in infant rats."Journal of Biomedical Materials Research. 54. 373-384 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kitahara A.K.: "Facial nerve repair using collagen nerve conduit"Scandinavian Journal of Plastic and Reconstru. Surg. And Hand Surg.. 33. 187-193 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Suzuki Y.: "In vivo evaluation of a novel alginate dressing"J of Biomed. Materials Res. (Applied Biomaterial). 48. 522-527 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Suzuki Y.: "Peripheral nerve regeneration across 50 mm gap repaired with a novel nerve guide composed of freeze-dried alginate gel"Neuroscience Letters. 259. 75-78 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yamawaki Y.: "Eustaian tube cartilage and medial movement of lateral pharyngeal wall on phonation"Plastic and Reconstructive Surgery.. 104. 350-356 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Suzuki Y.: "Regeneration of transected spinal cord in young adult rats using freeze-dried alginate gel"Neuroreport.. 10. 2891-2894 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Suzuki Y.: "Reconstruction of rat peripherol nerve gap without sutures using freeze-dried alginate gel"J of Biomed. Materials Res.. 49. 528-533 (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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