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Metabolism of Proteoglycans in the Extracellular Matrix

Research Project

Project/Area Number 11307040
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional basic dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

YANAGISHITA Masaki  Tokyo Medical and Dental University, Graduate School, Professor, 大学院・医歯学総合研究科, 教授 (70132793)

Co-Investigator(Kenkyū-buntansha) YOKOYAMA Miki  Tokyo Medical and Dental University, Graduate School, Lecturer, 大学院・医歯学総合研究科, 講師 (70191533)
KATARZYNA anna podyma  Tokyo Medical and Dental University, Faculty of Dentistry, Teaching Stuff, 歯学部, 教務職員 (90302877)
SHINOMURA Tamayuki  Tokyo Medical and Dental University, Graduate School, Assosiate Professor, 大学院・医歯学総合研究科, 助教授 (70206118)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥35,390,000 (Direct Cost: ¥33,500,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2001: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
Fiscal Year 2000: ¥6,300,000 (Direct Cost: ¥6,300,000)
Fiscal Year 1999: ¥20,900,000 (Direct Cost: ¥20,900,000)
Keywordsextracellular matrix / Proteoglycan / heparan sulfate / degradation enzyme / heparanase
Research Abstract

The extracellular matrix is one of the major constituents of connective tissues and functions as a molecular complex providing proper environments necessary for cell development, differentiation and metabolism. Together with collagens, proteoglycans play major constitutive roles in the extracellular matrix. We have studied the metabolic regulation of extracellular matrix, especially focusing on the catabolism of heparan sulfate proteoglycans and a degrading enzyme, heparanase, necessary for this process. We have isolated a large quantity of the enzyme from cell cultures of rat parathyroid cell line (PTr) and characterized its enzymatic properties in detail. We have determined the nucleotide sequence of the mRNA encoding heparanase. We also investigated roles of extracellular matrix degradation by heparanase in the mechanism of cancer cell invasion and metastasis, using clinical specimens of lung cancers, oral squamous cell carcinomas and esophageal squamous cell carcinomas. The major results are summarized as follow.
1. Heparanase, a specific enzyme which degrades heparan sulfate proteoglycans, was isolated and its enzymatic properties, including the substrate structural requirements were studied in detail.
2. Nucleotide sequence of the gene encoding heparanase was determined.
3. Monoclonal and polyclonal antibodies against heparanase have been prepared. Characterization of these antibodies is still under way by immunohistochemical and Western blot procedures.
4. Good correlation between the invasiveness and the production of heparanase in lung cancers, oral squamous cell carcinomas and esophageal squamous cell carcinomas has been established. Studies on roles of heparanase in the mechanism of cancer invasion are under way.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (82 results)

All Other

All Publications (82 results)

  • [Publications] San Miguel, S.M.: "Tissue-nonspecific alkaline phosphatase expression and alkaline phosphatase activity following application of retinoic acid in human dental pulp cell"Arch. Oral Biol.. 44. 861-869 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Goseki-Sone, M.: "Expression of mRNA Encoding Tissue-Nonspecific Alkaline Phosphatase in Human Dental Tissues"Calcif. Tissue Int.. 64. 160-162 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shibata, S.: "Histochemical localization of hyaluronan and versican in the rat molar dental pulp"Arch. Oral Biol.. 44. 373-376 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Zou, K.: "A heparin-binding growth factor, midkine, binds to a chondroitin sulfate proteoglycan, PG-M/versican"Eur. J. Biochem.. 267. 4046-4053 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Perissinotto, D.: "Avian neural crest cell migration is diversely regulated by the two major hyaluronan-binding proteoglycans PG-M/versican and aggrecan"Development. 127. 2823-2842 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shibata, S.: "Isolation of proteoglycan (versican) aggregate from rat dental pulp"Arch. Oral Biol.. 45. 563-568 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 池田美子: "顎顔面領域発生の調節におけるヘパラン硫酸の関与"口腔病学会雑誌. 67. 94 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sugiya, H.: "Ca^<2+>-regulated nitric oxide generation in rabbit parotid acinar cells"Cell Calcium. 30. 107-116 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katsumata, O.: "Association of FcγII with Low-density Detergent-resistant Membranes Is Important for Crosslinking-dependent Initiation of the Tyrosine Phosphorylation Pathway and Superoxide Generation"J. Immun.. 167. 5814-5823 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hara-Yokoyama, M.: "Complex Gangliosides as Cell Surface Inhibitors for the Ecto-NAD^+ Glycohydrolase of CD38"Biochemistry. 40. 888-895 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kaneko, S.: "Occiusal hypofunction causes a decrease of proteoglycans in the rat periodontal ligament"J. Periodont. Res.. 36. 9-17 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ogihara, Y.: "Biosynthesis of Proteoglycans in Bone and Cartilage of Parathyroid Hormone-related Peptide (PTHrP)-knockout mouse"J. Bone Miner. Metab.. 19. 4-12 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikuta, M.: "Expression of heparanase in oral cancer cell lines and oral cancer tissues"Oral Oncology. 37. 177-184 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mikami, S.: "Loss of Syndecan-1 and Increased Expression of Heparanase in Invasive Esophageal Carcinomas"Jpn. J. Cancer Res.. 92. 1062-1073 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 池田美子: "ラット初期胚におけるN-deacetylase/N-sulfotransferase (NDST)の発現"口腔病学会雑誌. 68. 184-192 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maruyama, K.: "Heparanase activity, expression of mRNA encoding MMP-2, MMP-9, MTI-MMP, TIMP-2, and invasive potentials of lung cancer cell lines"Akita J. Med.. 27. 191-199 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shibata, S.: "Developmental changes and regional differences in histochemical localization of hyaluronan and versican in postnatal molar dental pulp"Int. Endodont. J.. 35. 159-165 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohashi, K.: "Affinity binding of glycosaminoglycan with β_2-microblobulin"Nephron. 90. 158-168 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hishimoto-Uoshima, M.: "Effect of Interleukin-4 on the Proteoglycan Accumulation in Human Gingival Fibroblasts"J. Period. Pes.. 37. 42-49 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoneda, S.: "Biosynthesis of proteoglycans by rat dental pulp cells in culture with reference to effects of L-ascorbate"Arch. Oral Biol.. (印刷中). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoneda, M.: "New Frontiers in Medical Sciences : Redefining Hyaluronan"Elsevier Science. 382 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 柳下正樹: "基礎生化学実験法, 第5巻"東京化学同人. 338 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yanagishita, M.: "Proteoglycan Protocols"Humana Press. 558 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yanagishita, M.: "Glycosyltransferases and Related Genes"Springer. 670 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] San Miguel, S. M., Goseki-Sone, M., Sugiyama, E., Watanabe, H., Yanagishita, M. and Ishikawa, I.: "Tissue-nonspecific Alkaline phosphatase expression and alkaline phosphatase activity following application of retinoic acid in human dental pulp cells"Arch. Oral Biol.. 44. 861-869 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Goseki-Sone, M., Iimura, T., Takeda, K., Nifuji, A., Ogata, Y., Yanagishita, M. and Oida, S.: "Expression of mRNA Encoding Tissue-Nonspecific Alkaline Phosphatase in Human Dental Tissues"Calcif. Tissue Int.. 64. 160-162 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yanagishita, M.: "Proteoglycans in bone tissues. In "Osteoporosis - pathogenesis, diagnosis, prevention and therapy,""eds. Orimo, H., Suda,. 136-139 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shibata, S., Kaneko, S., Yanagishita, M. and Yamashita, Y.: "Histochemical localization of hyaluronan and versican in the rat molar dental pulp. Arch."Oral Biol. 44. 373-376 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Zou, K., Muramatu, H., Ikematsu, S., Sakuma, S., Salama, R.H., Shinomura, T., Kimata, K. and Muramatu, T.: "A heparin-binding growth factor, midkine, binds to a chondroitin sulfate proteoglycan, PG-M/versican."Eur. J. Biochem. 267. 4046-4053 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Perissinotto, D., Iacopetti, P., Bellina, I., Doliana, R., Colombatti, A., Pettway, Z., Bronner-Fraser, M., Shinomura, T., Kimata, K., Morgelin, M., Lofberg, J. and Perris, R: "Avian neural crest cell migration is diversely regulated by the two major hyaluronan-binding proteoglycans PG-M/versican and aggrecan."Development. 127. 2823-2842 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoneda, M., Zhao, M., Zhuo, L., Watanabe, H., Yamada, Y., Huang, L., Nagasawa, S., Nishimura, H., Shinomura, T., Isogai, Z. and Kimata, K.: "Roles of inter-α-trypsin inhibitor and hyaluronan-binding proteoglycans in hyaluronan-rich matrix formation"New Frontiers in Medical Sciences : Redefining Hyaluronan. pp21-30, ed. Abatangelo, G. and Weigel, P. H., r Elsevier Science B.V.. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shibata, S., Yoneda, S., Yanagishita, M. and Yamashita, Y.: "Isolation of proteoglycan (versican), aggregate from rat dental pulp"Arch. Oral. Biol.. 45. 563-568 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yanagishita, M., Podyma, Katarzyna Anna: "Metabolic labeling of heparan sulfate"In "Basic experimental procedures of biochemistry" vol. 5, ed. Taniguchi, N. pp. 305-307, Japanese Society of Biochemistry. (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikeda, M. and Yanagishita, M.: "Role of heparan sulfate proteoglycans in the craniofacial development"J. Stomatological Society (Japan). 67. 94 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Sugiya, H., Mitsui, Y., Michikawa, H., Fujita-Yoshigaki, J., Hara-yokoyama, M., Hashimoto, S. and Furuyama, S.: "Ca^<2+>-regulated nitric oxide generation in rabbit parotid acinar cells"Cell Calcium. 30. 107-116 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Katsumata, O., Hara-Yokoyama, M., Sautes-Fridman, C., Nagatsuka, Y., Katada, T., Hirabayashi, Y., Shimizu, K., Fujita-Yoshigaki, J., Sugiya, H. and Furuyama, S.: "Association of FcγII with Low-density Detergent-resistant Membranes Is Important for Crosslinking-dependent Initiation of the Tyrosine Phosphorylation Pathway and Superoxide Generation"J. Immun. 167. 5814-5823 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hara-Yokoyama, M., Nagatsuka, Y., Katsumata, O., Irie, F., Kontani, K., Hoshino, S., Katada, T., Ono, Y., Fujita-Yoshigaki, J., Sugiya, H., Furuyama, S. and Hirabayashi, Y.: "Complex Gangliosides as Cell Surface Inhibitors for the Ecto-NAD^+ Glycohydrolase of CD38"Biochemistry. 40. 888-895 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] yanagishita, M.: "Isolation of Proteoglycans from Cell Culture and Tissues : General Principles. In Proteoglycan Protocols"Current Methods and Applications (ed. Iozzo, R. V.). pp. 3-8, Humana Press, Philadelphia. (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yanagishita, M.: "Biosynthetic Pathways of Proteoglycans, in "Handbook of Glycosyltransferases and Related Genes," (eds. Taniguchi, N., Honke, K. and Fukuda, M.), Springer, Tokyo, 2001"

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kaneko, S., Ohashi, K., Soma, K. and Yanagishita, M: "Occlusal hypofunction causes a decrease of proteoglycans in the rat periodontal ligament"J. Period. Res.. 36. 9-17 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ogihara, Y., Suda, N., Senior, P. V., Hammond, V. E., Beck, F. and Yanagishita, M.: "Biosynthesis of Proteoglycans in Bone and Cartilage of Parathyroid Hormone-related Peptide (PTHrP)-knockout mouse"J. Bone Miner. Metab.. 19. 4-12 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikuta, M., Podyma, K. A., Maruyama, K., Enomoto, S. and Yanagishita, M.: "Expression of heparanase in oral cancer cell lines and oral cancer tissues"Oral Oncology. 37. 177-184 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mikami, S., Ohashi, K, Usui, Y., Nemoto, T., Katsube, K., Yanagishita, M., Nakajima, M., Nakamura, K. and Koike, M.: "Loss of Syndecan-1 and Increased Expression of Heparanase in Invasive Esophageal Carcinomas"Jpn. J. Cancer Res.. 92. 1062-1073 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ikeda, M.: "Expression of N-deacetylase/N-sulfotransferase (NDST) in rat early embryo"J. Stomatological Society (Japan). 68. 184-192 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maruyama, K.: "Heparanase activity, expression of mRNA encoding MMP-2, MMP-9, MTI-MMP, TIMP-2, and invasive potentials of lung cancer cell lines"Akita J. Med.. 27. 191-199 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Shibata, S., Yoneda, S., Yanagishita, M. and Yamashita, Y.: "Developmental changes and regional differences in histochemical localization of hyaluronan and versican in postnatal molar dental pulp"Int. Endodont. J.. 35. 159-165 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohashi, K., Kisilevsky, R. and Yanagishita, M.: "Affinity binding of glycosaminoglycan with β_2-microblobulin"Nephron. 90. 158-168 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hashimoto-Uoshima, M., Noguchi, K., Suzuki M. Murata, A. Yanagishita, M. and Ishikawa, I.: "Effect of Interleukin-4 on the Proteoglycan Accumulation in Human Gingival Fibroblasts"J. Period. Res.. 37. 42-49 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoneda, S., Shibata, S., Yamashita, T. and Yanagishita, M.: "Biosynthesis of proteoglycans by rat dental pulp cells in culture with reference to effects of L-ascorbate. (2002)"Arch. Oral Biol.. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Kaneko, S.: "Occlusal hypofunction causes a decrease of proteoglycans in the rat periodontal ligament"J. Period. Res.. 36. 9-17 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ogihara, Y.: "Biosynthesis of Proteoglycans in Bone and Cartilage of Parathyroid Hormone-related Peptide (PTHrP)-knockout mouse"J. Bone Miner. Metab.. 19. 4-12 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ohashi, K.: "Affinity binding of glycosaminoglycan with β_2-microblobulin"Nephron. 90. 158-169 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hashimoto-Uoshima, M.: "Effect of Interleukin-4 on the Proteoglycan Accumulation in Human Gingival Fibroblasts"J. Period. Res.. 37. 42-49 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ikuta, M.: "Expression of heparanase in oral cancer cell lines and oral cancer tissues"Oral Oncology. 37. 177-184 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mikami, S.: "Loss of Syndecan-1 and Increased Expression of Heparanase in Invasive Esophageal Carcinomas"Jpn. J. Cancer Res.. 92. 1062-1073 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yoneda, S.: "Biosynthesis of proteoglycans by rat dental pulp cells in culture with reference to effects of L-ascorbate"Arch. Oral Biol.. (in press). (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Hara-Yokoyama, M.: "Complex Gangliosides as Cell Surface Inhibitors for the Ecto-NAD+ Glycohydrolase of CD38"Biochemistry. 40. 888-895 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Katsumata, O.: "Association of FcgRII with low-density detergent-resistant membranes is important for cross-linking-dependent initiation of the tyrosine phosphorylation pathway and superoxide generation"J. Immunol.. 167. 5814-5823 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Sugiya, H.: "Ca2+-regulated nitric oxide generation in rabbit parotid acinar cells"Cell Calcium. 30. 107-116 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yanagishita, M.: "Proteoglycan Protocols"Humana Press. 558 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Yanagishita, M.: "Glycosyltransferases and Related Genes"Springer. 670 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Shibata,S.: "Isolation of Proteoglycon (versican) aggregate from rat dental pulp"Arch.Oral Biol.. 45. 563-568 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 池田美子: "顎顔面領域発生の調節におけるヘパラン硫酸の関与"口腔病学会雑誌. 67. 94 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kaneko,S.: "Occulusal hypofunction causes a decrease of proteoglycans in rat periodontal ligament."J.Period.Res. (in press). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ogihara,Y.: "Biosynthesis of proteoglycans in Bone and Cartilage of Parathyroid Hormone-related peptide knock-out mouse"J.Bone Miner,Metab.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ohashi,K.: "Affinity binding of heparin with β_2-microglobulin, and its enhancement of β_2 microglobulin amyloid fibrillogenesis"Am.J.Path.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ikuta,M.: "Expression of heparanase in oral cancer cell lines and oral tissnes"Oral Oncology. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hashimoto-Uoshima,M.: "Effect of Interleukin 4 on the Proteoglycan Accumulation in Human Gingival Fibroblasts"J.Period, Res.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 柳下正樹(共著): "新歯学大事典"永未書店(in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 柳下正樹(共著): "基礎生化学実験法第5巻"東京化学同人. 338 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yanagishita,M.(共著): "Proteoglycan Protocols"Humana Press (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yanagishita,M.(共著): "Handbook of Glycosyltransferases and Their Related"Springer Verlag (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shibata, S.: "Histochemical localization of hyaluronan and versican in the rat molor dental pulp"Arch. Oral Biol.. 44. 373-376 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] San Miguel, S.M.: "Tissue-nonspecific alkaline phosphatase expression and alkaline phosphatase activity following application of retinoic"Arch. Oral Biol.. 44. 861-869 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Goseki-Sone, M: "Expression of mRNA encoding tissue-nonspecific alkaline phosphatase in human dental Tissues"Calcif Tissue Int.. 64. 160-162 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shibata, S.: "Isolation of proteoglycan (versican) aggregate from rat dental pulp"Arch.Oral Biol.. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 池田美子: "顎顔面領域発生の調節におけるヘパラン硫酸の関与"口腔病学会雑誌. 67(印刷中). (2000)

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      1999 Annual Research Report
  • [Publications] Kaneko, S.: "Occulusal hypofunction causes a decrease of proteoglycans in the rat periodontal ligament"J. Period. Res. (印刷中). (2000)

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      1999 Annual Research Report
  • [Publications] 柳下正樹: "最新骨粗鬆症"ライフサイエンス出版. 640 (1999)

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      1999 Annual Research Report
  • [Publications] Yanagishita,M.: "Proteoglycan Protocols : Current Methods and Applications"Humana Press(印刷中). (2000)

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      1999 Annual Research Report
  • [Publications] 柳下正樹: "基礎生化学実験法,第5巻"日本生化学会(印刷中). (2000)

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  • [Publications] 柳下正樹: "新歯学大事典"永末書店(印刷中). (2000)

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      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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