• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development technique of bone regeneration using Mesenchymal Stem Cell

Research Project

Project/Area Number 11307048
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionNagoya University

Principal Investigator

UEDA Minoru  Nagoya University, Graduate School of Medicine, Professor, 大学院・医学研究科, 教授 (00151803)

Co-Investigator(Kenkyū-buntansha) OKAZAKI Yasuhiro  Nagoya University, Graduate School of Medicine, Assistant Professor, 大学院・医学研究科, 助手 (60313996)
HATA Ken-ichiro  Nagoya University, School of Medicine, Associate Professor, 医学部, 助教授 (80293710)
MIZUTANI Hideki  Nagoya University, Graduate School of Medicine, Associate Professor, 大学院・医学研究科, 助教授 (30167663)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥38,230,000 (Direct Cost: ¥37,000,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2001: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2000: ¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 1999: ¥28,900,000 (Direct Cost: ¥28,900,000)
KeywordsCultured Bone / Tissue Engineering / Marrow Cell / Carrier / Bone Regeneration / 組織再生 / 培養
Research Abstract

Recent studies in the field of tissue engineering have developed and established the technique of cultured bone regeneration with mesenchymal stem cells (MSCs) derived from bone marrow. Most of characteristics of the regenerat ion have been clarified by these recent studies. Generally, it have described a new technology called tissue engin eering that involves the morphogenesis of new tissue from constructs formed of isolated cells, biocompatible scaff olds and growth factors. In the present studies, we used MSCs as cell sources, ceramics and absorbable collagen as matrices, and rhBMP-2 and Platelet Rich Plasma as growth factors. In order to examine the effectiveness of cultur ed bone regeneration for clinical use, we investigated the following five things : 1) development of matrix, 2) ost eogenesis in vivo, 3) establishment of the method of larger scale of osteoblast culture, 4) the effectiveness of growth factors to improve the bone formation. 5) A possibility of clinical application in human. In regard to the matrix, we investigated the osteogenic potential of non resorbable HA and a new biodegradable β-tricalcium phosph ate (β-TCP), showing that β-TCP would be an ideal scaffolds for bone regeneration. In addition, we found that absorbable collagen would be an effective scaffold for rhBMP-2 in the sinus as well as autogenous bone. However, m ost studies regarding bone formation have adopted two dimensional histomorphometric evaluations and molecular biol ogys, not three dimensional such as micro CT. We also successfully evaluated microstructure of trabecular bone in human clinical cases. In some years from now, we are going to perform studies to improve the utility of the graft for the periodontal or alveolar defect for clinical use of technique of the cultured bone regeneration using MSCs with plasticity.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (43 results)

All Other

All Publications (43 results)

  • [Publications] Keisuke Wada: "Maxillary Sinus Floor Augmentation in Rabbits : A Comparative Histologic-Histomorphometric Study Between rhBMP-2 and Autogenous Bone"The International Journal of Periodontics & Restrative Dentistry. 21.3. 252-263 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masaki Honda: "Cartilage Formation by Cultured Chondrocytes in a New Scaffold Made of Poly (L-Lactide-Caprolactone) Spoge"Journal of Oral & Maxillofacial Surgery. 58. 767-775 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 夫 才成: "生体吸収性セラミックスβ-TCPを足場に用いた培養人工骨に関する実験的研究"日本口腔外科学会雑誌. 47.1. 1-7 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Minoru Ueda: "Tissue Engineering : applications for maxillofacial surgery"Materials Science and Engineering. C13. 7-14 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 山田陽一: "注入型培養骨"Clincal Calcium. 12.2. 50-54 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 上田 実: "人体再生"現代医学. 49.1. (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 松野 恵: "皮膚と骨-骨と皮膚の再生"外科. 63.3. 323-327 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 上田 実: "ティッシュエンジニアリングの最新動向-幹細胞を利用した骨再生"The Quintessence Year Book 2001. 10-14 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 本田雅規: "Tissue Engineeringによる軟骨組織再生"治療学. 35.1. 63-66 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 本田雅規: "ティッシュエンジニアリングによる軟骨修復"蛋白質 核酸 酵素. 45.13. 2297-2300 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 山田陽一: "骨形成細胞による骨再生"炎症と免疫. 9.3. 3-9 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 藤内 祝: "再生医学における移植組織の構築法"Surgery Frontier. 8.2. 86-90 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 上田 実: "骨・軟骨と再生医療"今日の移植. 14.51. 612-618 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 日比野祥敬: "生体組織工学の進歩"遺伝子医学. 4.2. 37-39 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 畠 賢一郎: "硬組織と皮膚の再生"現代医療. 31.12. 109-114 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Minoru Ueda: "Tissue Engineering for Therapeutic"Hard and soft tissue engineering : applications in implant surgery. 11 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Keisuke Wada: "Maxillary Sinus Floor Augmentation in Rabbits : A Comparative His oric Histomorphometric Study Between rhBMP and Autogenous Bone"The International Journal of Periodontics & Restrative Dentistry. 21.3. 252-263 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masaki Honda: "Cartilage Formation by Cultured Chondrocytes in a New Scaffold Made of Poly(I,-I, actide-ε-caprolaotone) sponge"Journal of Oral & Maxillofacial Surgely. 58. 767-775 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Saisei Fu: "Experimental study of cultured artificial bone using the bioahsorbable ceramic β-TCP as a scaffold"Japanese Journal of Oral & Maxillofacial Surgery. 47.1. 1-7 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mrnoru Ueda: "Tissue Engineering: applications for Maxillofacial Surgery"Materials Science and Engineering. C13. 7-14 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoiohi Yamada: "Tnjectable Bone"Clinical Calcium. 12.2. 50-54 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Minoru Ueda: "Human regeneration"The.Current Medicine. 49.1. 19-23 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Megumi Matuno: "Tissue and Bone - tissue and bone regeneratio"Surgery. 63.3. 323-327 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Minoru Ueda: "Recent Advance in Tissue Engineering : Bone Regeneration using Osteogenic Stem Cell"The Quintessence Year Book 2001. 10-14 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masaki Honda: "Cartilage Regeneration Using Tissue Engineering"Biomedicine & Therapeutic. 35-10. 63-66 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Masaki Honda: "Cartilage Regeneration Using Tissue Engineering"Protein, Neucric acid and Enzyme. 45.13. 2297-2300 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoichi Yamada: "Bone Regeneration Using Osteogenic Stem Cell"Inflammation and Immunology. 9.3. 3-9 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Twai Tounai: "Transplantation Using Tissue Engineering"Surgery Frontier. 8.2. 86-90 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Minoru Ueda: "Tissue Engineering in Bone and Cartilage"Transplant Today. 14.5. 612-618 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshitaka Hibino: "The Progress of Tissue Engineering -Regeneration of the Bone and Cartilages"gene and Medicine. 4.2. 37-39 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ken-ichiro Hata: "Regeneration of Hard tissue and Skin"New Horizon for Medicine. 31.12. 109-114 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Minoru Ueda: "Hard and soft tissue engineering: applications in implant surgery"Tissues Engineering for Therapeu tic. 191-202 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Keisuke Wada: "Maxillary Sinus Floor Augmentation in Rabbits : A Comparative Histologic-Histomorphometric Study Between rhBMP-2 and Autogenous Bone"The International Journal of Periodontics & Restrative Dentistry. 21.3. 252-263 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 山田陽一: "注入型培養骨"Clincal Calcium. 12.2. 50-54 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 上田 実: "人体再生"現代医学. 49.1. 19-23 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 松野 恵: "皮膚と骨-骨と皮膚の再生"外科. 63.3. 323-327 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 上田 実: "ティッシュエンジニアリングの最新動向-幹細胞を利用した骨再生"The Quintessence Year Book 2001. 10-14 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 本田雅規: "Tissue Engineeringによる軟骨組織再生"治療学. 35.10. 63-66 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 山田陽一: "骨形成細胞による骨再生"炎症と免疫. 9.3. 3-9 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 藤内 祝: "再生医学における移植組織の構築法"Surgery Frontier. 8.2. 86-90 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 上田 実: "骨・軟骨と再生医療"今日の移植. 14.5. 612-618 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Minoru Ueda: "Tissue Engineering for Therapeutic"Hard and soft tissue engineering : applications in implant surgery. 11 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 夫才成,岡崎恭宏,畠賢一郎,上田実 他: "生体吸収性セラミックスβ-TCPを足場に用いた培養人工骨に関する実験的研究"日本口腔外科学会誌. 47巻1号. 1-7 (2001)

    • Related Report
      2000 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi