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Molecular evolution and G protein subtype specificity of G protein coupled receptors

Research Project

Project/Area Number 11308027
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Biophysics
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

SHICHIDA Yoshimori  Dept. Biophys, Grad. sh. Sci., Professor, 大学院・理学研究科, 教授 (60127090)

Co-Investigator(Kenkyū-buntansha) TERAKITA Akihisa  Dept. Biophys, Grad. sh. Sci., Associate Professor, 大学院・理学研究科, 助教授 (30212062)
Project Period (FY) 1999 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥38,980,000 (Direct Cost: ¥36,700,000、Indirect Cost: ¥2,280,000)
Fiscal Year 2002: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2001: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2000: ¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 1999: ¥14,900,000 (Direct Cost: ¥14,900,000)
Keywordsrhodopsim / G protein coupled receptor / crystal structure / G protein / G protein specificity / chimeric mutant / biochemistry / molecular evolution / 2次元結晶解析 / ムスカリン受容体
Research Abstract

One of the motifs of signal transduction systems widely present in organisms is the [Receptor→G protein→Enzyme]. The receptor (G protein-coupled receptor, GPCR) which participates in this system has seven-transmembrane α-helical structure, and 2000 or more kinds are discovered so far.
GPCR have been variously diversified in the course of molecular evolution, and they support organisms working as sophisticated information processing systems. In the present study, we have investigated the functional expression of GPCR using a rhodopsin as a model GPCR for furthering our understanding of the common mechanism of G protein activation by GPCR and its diversification.
Consequently, it was revealed that the fundamental molecular architectures for activating G protein are similar among GPCRs belonging to a rhodopsin super family (family 1). On the other hand, comparison of rhodopsin with metabotropic glutamate receptor (mGluR) suggested that the molecular architectures of rhodopsin and mGluR related to G protein interaction are different but the G protein activation mechanism involving the cytoplasmic loops is common. Moreover, the structural changes of rhodopsin in the process from photoreception to G protein activation were investigated by means of various spectroscopic and molecular biological techniques. Consequently, it was elucidated the mechanism of helical movements induced by the light-activated retinal chromophore, and identified the amino acid residues which control the rate of structural changes in rhodopsin molecule.
Furthermore, the three-dimensional structure of rhodopsin was explored and seven water molecules were identified in the molecule. It was also found that the counterion which are important for absorption of a visible light have been diversified among the groups of rhodopsin family.

Report

(5 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (114 results)

All Other

All Publications (114 results)

  • [Publications] H.Imai: "Probing for the threshold energy for visual transduction : Red-shifted visual pigment analogs from 3-methoxy-3-dehydroretinal and related compounds"Photochem.Photobiol.. 70. 111-115 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Imamoto: "Anion binding effect to iodopsin studied by low-temperature FTIR spectroscopy"Biochemistry. 38. 11749-11754 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Kandori: "Structural change of threonine 89 upon photoisomerization in bacteriorhodopsin as revealed by polarized FTIR spectroscopy"Biochemistry. 38. 9676-9683 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A.Nakamura: "Chimeric nature of pinopsin between rod and cone visual pigments"Biochemistry. 38. 14738-14745 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A.Onishi: "Dichromatism in macaque monkeys"Nature. 402. 139-140 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Kandori: "Local and distant protein structural changes on photoisomerization of the retinal in bacteriorhodopsin"Proc Natl.Acad.Sci USA. 97. 4643-4648 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Shichida: "Visual pigment : Photochemistry and molecular evolution"The Retinal Basis of Vision.(eds.J.Toyoda et al.)(Elsevier Science, The Netherlands). 15 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] B.Borhan: "Movement of retinal along the visual transduction path"Science. 288. 2209-2212 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Yamashita: "Distinct roles of the second and the third cytoplasmic loops of bovine rhodopsin in G protein activation"J Biol.Chem.. 275. 34272-34279 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A.Terakita: "Highly conserved glutamic acid in the extracellular IV-V loop in rhodopsins acts as the counterion in retinochrome, a member of rhodopsin family"Proc Natl.Acad.Sci USA. 97. 14263-14267 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Kandori: "Direct observation of the bridged water stretching vibrations inside a protein"J.Am.Chem.Soc.. 122. 11745-11746 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S.Nishimura: "Activation mechanisms of G protein by seven-transmembrane receptors : A view from rhodopsin studies"J.Indian Chem.Soc.. 77. 665-676 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Kandori: "Photoisomerization in rhodopsin"Biochemistry (Moscow). 66. 1197-1209 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Shichida: "Amino acid residues controlling the properties and functions of rod and cone visual pigments"Rhodopsins and Phototransduction. (ed. Novartis Foundation)(John Wiley & Sons, England). 12 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Hirano: "Chloride effect on iodopsin studied by low-temperature visible and infrared spectroscopies"Biochemistry. 40. 1385-1392 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Imai: "Difference in molecular structure of rod and cone visual pigments studied by Fourier transform infrared spectroscopy"Biochemistry. 40. 2879-2786 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A.Nakamura: "Regulatory mechanism for stability of meta II intermediate of pinopsin"J.Biochem (Tokyo). 129. 329-334 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Kandori: "Excited-state dynamics of rhodopsin probed by femtosecond fluorescence spectroscopy"Chem.Phys.Lett.. 334. 271-276 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Kandori: "Tight Asp85-Thr89 association during the pump switch of bacteriorhodopsin"Proc.Natl.Acad.Sci.USA. 98. 1571-1576 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] N.Katagiri: "Demonstration of a rhodopsin-retinochrome system in the stalk eye of a marine gastropod Onchidium by immunohistochemistry"J.Comp.Neurology. 433. 380-389 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Nakagawa: "O-Glycosylation of G-protein-coupled receptor, octopus rhodopsin. Direct analysis by FAB mass spectrometry"FEBS Lett.. 496. 19-24 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Yamashita: "The second cytoplasmic loop of metabotropic glutamate receptor functions at the third loop position of rhodopsin"J.Biochem.(Tokyo). 130. 149-155 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Kandori: "Structural changes of pharaonis phoborhodopsin upon photoisomerization of the retinal chromophore : Infrared spectral comparison with bacteriorhodopsin"Biochemistry. 40. 9238-9246 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A.Wada: "Retionids and related compounds. Part 26. Synthesis of (11-Z)-8, 18-propano-and methano-retinals and conformational study of the rhodopsin choromophore"J.Chem.Soc., Perkin Trans. 1. 2430-2439 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Kandori: "Internal water molecules of pharaonis phoborhodopsin studied by low-temperature infrared spectroscopy"Biochemistry. 40. 15693-15698 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A.Terakita: "Functional interaction between bovine rhodopsin and G protein transducin"J.Biol.Chem.. 277. 40-46 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A.Onishi: "Variations in long-and middle-wavelength-sensitive opsin gene loci in crab-eating monkeys"Vision Res.. 42. 281-292 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Nagata: "Isomer-specific interaction of the retinal chromophore with threonine-118 in rhodopsin"J.Phys.Chem.. 106. 1969-1975 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Kandori: "Interaction of Asn105 with the retinal chromophore during photoisomerization of pharaonis phoborhodopsin"Biochemistry. 41. 4554-4559 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Okada: "Functional role of internal water molecules in rhodopsin revealed by X-ray crystallography"Proc.Natl.Acad.Sci.USA. 99. 5982-5987 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Ueyama: "Novel missense mutations in red/green opsin genes in congenital color-vision deficiencies"Biochem.Biophys.Res.Commun.. 294. 205-209 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M.Koyanagi: "Amphioxus homologs of Go-coupled rhodopsin and peropsin having 11-cis-and all-trans-retinals as their chromophores"FEBS Letters. 531. 525-528 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T.Hirano: "Constraints of Opsin Structure on the Ligand Binding Site : Studies with Ring-fused Retinals"Photochem. and Photobiol.. 76. 606-615 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S.Kuwayama: "Conserved Proline Residue at Position 189 in Cone Visual Pigments as a Determinant of Molecular Properties Different from Rhodopsin"Biochemistry. 41. 15245-15252 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H.Imai: "Analysis of amino acid residues in rhodopsin and cone visual pigments that determine their molecular properties. Methods Enzymol"Academic Press, U.S.A.. 21 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y.Shichida: "Heterogeneity of rhodopsin intermediates states interacting with transducin. Methods Enzymol"Academic Press, U.S.A.. 18 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] 七田芳則: "視物質からみた光情報伝達系の多様性「遺伝子で生物の進化を考える」第3回「大学と科学」公開シンポジウム組織委員会編"クバプロ. 12 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Imai, T. Hirano, A. Terakita, Y. Shichida, R. Muthyala, R. -L. Chen, L. U. Colmenares and R. S. H. Liu: "Probing for the threshold energy for visual transduction: Red-shifted visual pigment analogs from 3-methoxy-3-dehydroretinal and related compounds"Photochem. Photobiol.. 70 70. 111-115 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Y. hamoto, T. Hirano, H. Imai, H. Kandori, A. Maeda, T. Yoshizawa, M. Groesbeek, J. Lugtenburg and Y. Shichida: "Anion binding effect to iodopsin studied by low-temperature FTIR spectroscopy"Biochemistry. 38. 11749-11754 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Kandori, N, Kinoshita, Y. Yamazaki, A. Maeda, Y. Shichida R. Needleman, J. K. Lanyi, M. Bizounok J. Herzfeld, J. Raap and J. Lugtenburg: "Structural change of threonine 89 upon photoisomerization in bacteriorhodopsin as revealed by polarized FTIR spectroscopy"Biochemistry. 38. 9676-9683 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A. Nakamura, D. Kojima, H. Imai, A. Terakita, T. Okano, Y. Shichida and Y. Fukada: "Chimeric nature of pinopsin between rod and visual pigments"Biochemistry. 38. 14738-14745 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A. Onishi, S. Koike, M. Ida, H. Imai, Y. Shichida, O. Takenaka, A. Hanazawa, H. Komatsu, A. Komatsu, S. Goto, B. Suryobroto, K. Kitahara and T. Yamamori: "Dichromatism in macaque monkeys"Nature. 402. 139-140 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Kandori, N. Kinoshita Y. Yamazaki, A. Maeda, Y. Shichida, R. Needleman, J. K. Lanyi, M. Bizounok, J. Herzfeld, J. Raap, and J. Lugtenburg: "Local and distant protein structural changes on photoisomerization of the retinal in bacteriorhodopsin"Proc Natl. Acad. Sci USA.. 97. 4643-4648 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] B. Borhan, M. L. Souto, H. Imai, Y. Shichida, and K. Nakanishi: "Movement of retinal along the visual transduction path"Science. 288(5474). 2209-2212 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Yamashita, A. Terakita, and Y. Shichida Y.: "Distinct roles of the second and the third cytoplasmic loops of bovine rhodopsin in G protein activation"J Biol. Chem.. 275. 34272-34279 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A. Terakita, T. Yamashita, and Y. Shichida: "Highly conserved glutamic acid in the extracellular IV-V loop in rhodopsins acts as the counterion in retinochrome, a member of rhodopsin family"Proc. Natl. Acad. Sci. USA. 97. 114263-14267 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Kandori and Y. Shichida: "Direct observation of the bridged water stretching vibrations inside a protein"J. Am. Chem. Soc.. 122. 11745-11746 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S. Nishimura and Y. Shichida: "Activation mechanisms of G protein by seven-transmembrane receptors: A view from rhodopsin studies"J. Indian Chem. Soc.. 77. 665-676 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Kandori, Y. Shichida and T. Yoshizawa: "Photoisomerization in rhodopsin"Biochemishy(Moscow). 66(11). 1197-209 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Hirano, H. Imai, H. Kandori and Y. Shichida: "Chloride effect on iodopsin studied by low-temperature visible and infrared spectroscopies"Biocheistry. 40. 1385-1392 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Imai, T. Hirano, H. Kandori, A. Terakita and Y. Shichida: "Difference in molecular structure of rod and cone visual pigments studied by Fourier transform infrared spectroscopy"Biochemistry. 40. 2879-2786 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A. Nakamura, D. Kojima, T. Okano, H. Imai, A. Terakita, Y. Shichida and Y. Fukada: "Regulatory mechanism for stability of meta II intermediate of pinopsin"J. Biochem(Tokyo). 129. 329-334 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Kandori, Y. Furutani, S. Nishimura, Y. Shichida, H. Chosrowjan, Y. Shibata and N. Mataga: "Excited-state dynamics of rhodopsin probed by femotosaond fluorescence spechoscopy"Chem. Phys. Lett.. 334. 271-276 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Kandori, Y. Yarnazaki, Y. Shichida, J. Raap, J. Lugtenburg, M. Belenky and J. Herzfeld: "Tight Asp85-Thr89 association during the pump-switch of bacteriorhodopsin"Proc. Natl. Acad. Sci. USA.. 98. 1571-1576 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] N. Katagiri, A, Terakita, Y. Shichida and Y. Katagiri: "Demonstration of a rhodopsin-retinochrome system in the stalk eye of a marine gastropod Onchidium by immunohistochemistry"J. Comp. Neurology.. 433. 380-389 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M. Nakagawa, T. Miyamoto, R. Kusakabe, S. Takasaki, T. Takao, Y. Shichida, Tsuda M.: "O-Glycosylation of G-protein-coupled receptor, octopus rhodopsin. Direct analysis by FAB mass spechometry"FEBS Lett.. 496. 19-24 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Yamashita, A. Terakita and Y. Shichida: "The second cytoplasmic loop of metabotropic glutamate receptor functions at the third loop position of rhodopsin"J. Biochem. (Tokyo). 130. 149-155 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Kandori, K. Shimono, Y. Sudo, M. Iwamoto, Y. Shichida and N. Kamo: "Structural changes of pharaonis phoborhodopsin upon photoisomerization of the retinal chromophore: Infrared spectal comparison with bacteriorhodopsin"Biochemistry. 40. 9238-9246 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A. Wada, M. Tsutsumi, Y. Inatomi, H. Imai, Y. Shichida and M. Ito: "Retionids and related compounds. Part26. Synthesis of(11-Z)-8, 18-propano- and methano-retinals and conformational study of the rhodopsin choromophore"J. Chem. Soc. , Perkin Trans. 1. 2430-2439 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Kandori, Y. Furutani, K. Shimono, Y. Shichida and N. Kamo: "Internal water molecules of pharaonis phoborhodopsin studied by low-temperature infrared spectroscopy"Biochemistry. 40(51). 15693-15698 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A. Terakita, T. Yamashita, N. Nimbari, D. Kojima and Y, Shichida: "Functional interaction between bovine rhodopsin and G protein transducin"J. Biol. Chem.. 277(1). 40-46 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] A. Onishi, S. Koike, M. Ida-Hosonuma, H. Imai, Y. Shichida, O. Takenaka, A. Hanazawa, H. Komatsu, A. Mikami, S. Goto, B. Surybroto, A. Farajallah, P. Varavudhi, K. Kitahara and T. YamamoriVariations in long-and middle-wavelength-sensitive opsin gene loci in crab-eating monkeys.: "Variations in long- and middle-wavelength-sensitive opsin gene loci in crab-eating monkeys"Vision Res.. 42(3). 281-292 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Nagata, Y. Oura, A. Terakita, H. Kandori and Y. Shichida: "Isomer-specific interaction of the retinal chromophore with threonine-118 in rhodopsin"J. Phys. Chem.. 106(10). 1969-1975 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Kandori, K. Shimono, Y, Shichida, and N. Kamo: "Intraction of Asn105 with the retinal chromophore during photoisomerization of pharaonis phoborhodopsin"Biochemistry. 41(14). 4554-4559 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Okada, Y. Fujiyoshi, M. Silow, J. Navarro, E. M. Ltndau and Y. Shichida: "Functional role of intemal water molecules in rhodopsin revealed by X-ray crystal lography"Pros. Natl. Acad. Sol. USA. 99(9). 5982-5987 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] H. Ueyama, S. Kuwayama, H. Imai, S. Tanabe, S. Oda, Y. Nishida, A. Wada, Y. Shichida, and S. Yamade: "Novel missense mutations in red/green opsin genes in congenital color-vision deficiencies"Biochem. Biophys. Res. Commun.. 294(2). 205-209 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] M. Koyanagi, A. Terakita, K. Kubokawa and Y. Sbichida: "Amphioxus homologs of Go-coupled rhodopsin and peropsin having 11-cis- and all-trans-retinals as their chromophores"FEBS Letters. 531. 525-528 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] T. Hirano, I. T. Lim, D. M. Kim, X. -G. Zheng, K. Yoshihara, Y. Oyama, H. Imai, Y. Shichida and M. Ishiguro: "Constraints of Opsin Sbcture on the Ligand Binding Site: Studies with Ring-fused Retinals"Photochem. and Photobiol.. 76(6). 606-615 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S. Kuwayama, H. Imai, T. Hirano, A. Terakita and Y. Shichida: "Conserved Proline Residue at Position 189 in Cone Visual Pigments as a Dekrminant of Molecular Properties Different from Rhodopsin"Biochemistry. 41. 15245-15252 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] S.Kuwayama: "Conserved Proline Residue at Position 189 in Cone Visual Pigments as a Determinant of Molecular Properties Different from Rhodopsin"Biochemistry. 41. 15245-15252 (2002)

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      2002 Annual Research Report
  • [Publications] H.Kandori: "Interaction of Asn105 with the retinal chromophore during photoisomerization of pharaonis phoborhodopsin"Biochemistry. 41. 4554-4559 (2002)

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      2002 Annual Research Report
  • [Publications] T.Okada: "Functional role of internal water molecules in rhodopsin revealed by X-ray crystallography"Proc. Natl. Acad. Sci. USA. 99. 5982-5987 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] H.Ueyama: "Novel missense mutations in red/green opsin genes in congenital color-vision deficiencies"Biochem. Biophys. Res. Commun.. 294. 205-209 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] M.Koyanagi: "Amphioxus homologs of Go-coupled rhodopsin and peropsin having 11-cis-and all-trans-retinals as their chromophores"FEBS Letters. 531. 525-528 (2002)

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      2002 Annual Research Report
  • [Publications] T.Hirano: "Functional role of internal water molecules in rhodopsin revealed by X-ray crystallography"Photochem. and Photobiol.. 76. 606-615 (2002)

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  • [Publications] 七田芳則: "色覚(生物物理学ハンドブック)"朝倉書店(印刷中). (2003)

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  • [Publications] H.lmai: "Difference in molecular structure of rod and cone visual pigments studied by Fourier transform infrared spectroscopy"Biochemistry. 40. 2879-2886 (2001)

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      2001 Annual Research Report
  • [Publications] A.Nakamura: "Regulatory mechanism for the stability of the meta II intermediate of pinopsin"J.Biochem.(Tokyo). 129. 329-334 (2001)

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  • [Publications] H.Kandori: "Exited-state dynamics of rhodopsin probed by femtosecond fluorescence spectroscopy"Chem.Phys.Lett.. 334. 271-276 (2001)

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  • [Publications] N.Katagiri: "Demohstration of a rhodopsin-retinochrome system in the stalk eye of a marine gastropod, Onchidium, by immunohistochemistry"J.Comp.Neurol.. 433. 380-389 (2001)

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  • [Publications] M.Nakagawa: "O-Glycosylation of G-protein-coupled receptor, octopus rhodopsin. Direct analysis by FAB mass spectrometry"FEBS Lett.. 496. 19-24 (2001)

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      2001 Annual Research Report
  • [Publications] A.Wada: "Retionids and related compounds. Part26. Synthesis of(11-Z)-8, 18-propano-and methano-retinals and conformational study of the rhodopsin choromophore"J.Chem.Soc., Perkin Trans. 1. 2430-2439 (2001)

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  • [Publications] T.Yamashita: "The second cytoplasmic loop of metabotropic glutamate receptor functions at the third loop position of rhodopsin"J.Biochem.(Tokyo). 130. 149-155 (2001)

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  • [Publications] H.kandori: "Structural changes of pharaonis phoborhodopsin upon photoisomerization of the retinal chromophore : infrared spectral comparison with bacteriorhodopsin"Biochemistry. 40. 9238-9246 (2001)

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  • [Publications] H.kandori: "Photoisomerization in rhodopsin"Biochemistry(Mosc). 66. 1197-1209 (2001)

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  • [Publications] H.kandori: "lnternal water molecules of pharaonis phoborhodopsin studied by low-temperature infrared spectroscopy"Biochemistry. 40. 15693-15698 (2001)

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  • [Publications] A.Terakita: "Functional interaction between bovine rhodopsin and G protein transducin"J.Biol.Chem.. 277. 40-46 (2002)

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  • [Publications] A.Onishi: "Variations in long-and middle-wavelength-sensitive opsin gene loci in crab-eating monkeys"Vision Res.. 42. 281-292 (2002)

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  • [Publications] T.Nagata: "Isomer-specific interaction of the retinal chromophore with threonine-118 in rhodopsin"J.Phys.Chem. (in press). (2002)

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  • [Publications] 七田芳則: "ロドプシンの光情報変換過程-立体構造の解明により見えてきたメカニズム-"ビタミン. 75. 300-305 (2001)

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  • [Publications] 七田芳則: "視物質はいかにして多様化したのか-分子進化から探る-"現代化学. 362. 55-62 (2001)

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  • [Publications] 平野貴弘: "視物質における吸収波長制御機構-アニオンによる制御を中心として-"生物物理. 41. 284-289 (2001)

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  • [Publications] 七田芳則: "生物物理学とはなにか「受容体」(日本生物物理学会編)"共立出版(印刷中). (2001)

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  • [Publications] H.Imai: "Difference in molecular structure of rod and cone visual pigments studied by Fourier transform infrared spectroscopy"Biochemistry. (in press). (2001)

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  • [Publications] H.Kandori: "Excited-state dynamics of rhodopsin probed by femtosecond fluorescence spectroscopy"Chem.Phys.Lett. (in press). (2001)

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  • [Publications] H.Kandori: "Tight Asp85-Thr89 association during the pump switch of bacteriorhodopsin"Proc.Natl.Acad.Sci.USA. 98(4). 1571-1576 (2001)

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  • [Publications] T.Hirano: "Chloride effect on iodopsin studied by low-temperature visible and infrared spectroscopies"Biochemistry. 40(5). 1385-1392 (2001)

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  • [Publications] H.Kandori: "Direct observation of the bridged water stretching vibrations inside a protein"J.Am.Chem.Soc. 122. 11745-11746 (2000)

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  • [Publications] A.Terakita: "Highly conserved glutamic acid in the extracellular IV-V loop in rhodopsins acts as the counterion in retinochrome, a member of rhodopsin family"Proc.Natl.Acad.Sci.USA.. 97. 14263-14267 (2000)

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  • [Publications] T.Yamashita: "Distinct roles of the second and the third cytoplasmic loops of bovine rhodopsin in G protein activation"J.Biol.Chem.. 275. 34272-34279 (2000)

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  • [Publications] B.Borhan: "Movement of retinal along the visual transduction path"Science. 288. 2209-2212 (2000)

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  • [Publications] H.Kandori: "Local and distant protein structural changes on photoisomerization of the retinal in bacteriorhodopsin"Proc Natl.Acad.Sci USA.. 97. 4643-4648 (2000)

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  • [Publications] S.Nishimura: "Activation mechanisms of G protein by seven-transmembrane receptors : A view from rhodopsin studies"J.Indian Chem.Soc.. (in press). (2001)

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  • [Publications] H.Imai: "Methods Enzymol."Academic Press. 959 (2000)

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      2000 Annual Research Report
  • [Publications] Y.Shichida: "Methods Enzymol."Academic Press. 959 (2000)

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  • [Publications] 七田芳則: "生き物はどのように世界を見ているか"学会出版センター(印刷中).

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  • [Publications] H.Imai: "Probing for the threshold energy for visual transduction: Red-shifted visual pigment analogs from 3-methoxy-3-dehydroretinal and related compounds"Photochem Photobiol.. 70,. 111-115 (1999)

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  • [Publications] Y.Imamoto: "Anion binding effect to iodopsin studied by low-temperature FTIR spectroscopy"Biochemistry. 38. 11749-11754 (1999)

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  • [Publications] H.Kandori: "Structural change of threonine 89 upon photoisomerization in bacteriorhodopsin as revealed by polarized FTIR spectroscopy"Biochemistry. 38. 9676 (1999)

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  • [Publications] A.Nakamura: "Chimeric nature of pinopsin between rod and cone visual pigments"Biochemistry. 38. 14738-14745 (1999)

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      1999 Annual Research Report
  • [Publications] A.Onishi: "Dichromatism in macaque monkeys"Nature. 402. 139-140 (1999)

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  • [Publications] H.Kandori: "Local and distant protein structural changes upon photoisomerization of the retinal in bacteriorhodopsin"Proc. Natl. Acad. Sci. USA. (in press). (2000)

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      1999 Annual Research Report
  • [Publications] Y. Shichida: "The Retinal Basis of Vision. (eds. J. Toyoda et al.)"Elsevier Science, The Netherlands.. 290 (1999)

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  • [Publications] Y. Shichida: "Rhodopsins and Phototransduction."John Wiley &. Sons, England.. 306 (1999)

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Published: 1999-04-01   Modified: 2016-04-21  

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