| Project/Area Number |
11356004
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | TOKYO INSTITUTE OF TECHNOLOGY |
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Principal Investigator |
KAKINUMA Katsumi TOKYO INSTITUTE OF TECHNOLOGY, GRADUATE SCHOOL OF SCIENCE AND ENGINEERING, PROFESSOR, 大学院・理工学研究科, 教授 (90092543)
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| Project Period (FY) |
1999 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥37,860,000 (Direct Cost: ¥33,600,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2002: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2001: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
Fiscal Year 2000: ¥7,100,000 (Direct Cost: ¥7,100,000)
Fiscal Year 1999: ¥12,300,000 (Direct Cost: ¥12,300,000)
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| Keywords | sugar-carbocyclization enzymes / aminoglycoside antibiotics / metabolic engineering / 2-deoxy-scyllo-inosose / mechanism-based enzyme inhibitor / benzenoid resources / carbaglucose / secondary metabolism enzymes / 糖質還化酵素 / カナマイシン / アミノ基転移酵素 / 基質認識 / 部位特異的変異 / 糖質環化 / スーデオキシ-scyllo-イノソース合成酵素 / 反応機構 / カテコール / 持続可能資源 / バイオマス |
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| Research Abstract |
New paradigm for manufacturing sustainable chemical resources based on the CO_2 fixation is highly desirable to conserve fossil resources and to protect our living environments. An approach using carbohydrate-cyclizing enzyme, 2-deoxy-scyllo-inosose (DOI) synthase (DOIS), being originally involved in the biosynthesis of butirosins in Bacillus circulans, has been developed, and detailed analysis and alternative application of DOIS have been exploited. (1) DOIS of B. circulans was purified, and the responsible gene (btrC) was identified and expressed in E. coli. (2) Fermentation conditions of the transformed E. coli and preparative protocol of DOI were established. (3) Conversion of DOI into catechol or 1, 2, 4-triacetoxybenzene was confirmed by simple chemical treatment. (4) Detailed interaction between the enzyme and substrate was analyzed using substrates analogues to elucidate importance of specific hydrogen-bondings. (3) Active site mapping was pursued with mechanism-based inhibitor
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with aids of enzymatic digestion and LC/ESI/MS/MS analysis. (4) For alternative preparation of DOI by metabolic engineering, the biosynthetic gene cluster of butirosin was extensively analyzed. (5) A new transamination enzyme (BtrS) was identified to be responsible for the step next to DOIS, and was over-expressed in E. coli to confirm the function. (6) The btrS gene in B. circulans was disrupted by in frame deletion. Potential of the disruptant for DOI production is currently under investigation. (7) The over-expressed BtrS was utilized to prepare.2-deoxy-scyllo-inosamine as a potential resource for the development of pharmaceuticals. (8) DOI was successfully converted into carbaglucose which is a promising substitute for glucose without dietary load. (8) A new DOIS was identified from a Streptomyces sp. and overexpressed. Its function was confirmed. All these works appear to constitute significant fundamentals for the precise understanding of DOISs and for its application to the preparation of organic resources. Less
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