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Screening for signal-responsive genes by an enhancer-trap vector and their application

Research Project

Project/Area Number 11357002
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section展開研究
Research Field General medical chemistry
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

NODA Makoto  GRADUATE SCHOOL OF MEDICINE, KYOTO UNIVERSITY, PROFESSOR, 医学研究科, 教授 (30146708)

Co-Investigator(Kenkyū-buntansha) KITAYAMA Hitoshi  GRADUATE SCHOOL OF MEDICINE, KYOTO UNIVERSITY, ASSISTANT PROFESSOR, 医学研究科, 助教授 (30231286)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥31,500,000 (Direct Cost: ¥29,700,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2001: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Fiscal Year 2000: ¥9,800,000 (Direct Cost: ¥9,800,000)
Fiscal Year 1999: ¥13,900,000 (Direct Cost: ¥13,900,000)
Keywordsretroviral vector / TGF-β / TMX / thioredoxin / ER stress / bleomycin / c-IAP2 / X-ray-responsive genes / レトロウイルスベクター / エンハンサートラップ / X線 / NF-kB / Bax / 遺伝子発現 / エンハンサー・トラップ / シグナル伝達 / TGF-β
Research Abstract

As applications of the newly developed enhancer trap system using a retroviral vector (pROSA-nGBT), we screened genes responsive to TGF-β or bleomycin, succeeded in isolating multiple genes of interest in each case, and could publish a part of these results. One of the TGF-β-responsive genes was found to encode a novel, thioredoxin-related transmembrane protein localized in the endoplasmic reticulum (ER) and was suggested to be a molecule involved in the management of ER stress. One of the genes responsive to bleomycin, a drug inducing DNA double strand break, was identified as C-IAP2 know to be involved in apoptosis. Since the promoter of this gene was found also to respond to therapeutic dosages of ionizing radiation, its application in radiation gene therapy is an interesting possibility and warrants further investigation.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] W.Matsuo: "Identifiaction of a novel thioredoxin-related transmembrane protein"J. Biol. Chem.. 276. 10032-10038 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Ueda: "c-IAP2 is induced by ionizing radiation through NF-kB binidng sites"FEBS Lett.. 491. 40-44 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T. Ueda: "c-IAP2 is induced by ionizing radiation through NF-kB binidng sites"FEBS Lett.. 491. 40-44 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y. Matsuo: "Identifiaction of a novel thioredoxin-related transmembrane protein"J. Biol. Chem. 276. 10032-10038 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.Matsuo: "Identifiaction of a novel thioredoxin-related transmembrane protein"J.Biol.Chem.. 276. 10032-10038 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] T.Ueda: "c-IAP2 is induced by ionizing radiation through NF-kB biniding sites"FEBS Lett.. 491. 40-44 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 松尾禎之 ら: "Identifiaction of a novel thioredoxin-related transmembrane protein"J.Biol.Chem.. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] 上田俊彦 ら: "c-IAP2 is induced by ionizing radiation through NF-kB binidng sites"FEBS Lett.. 491. 40-44 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 今村行雄 ら: "Roles of GABAergic inhibition and NMDA receptor subunits in the spatio-temporal integration in the cerebellar cortex of mice"Neurosci.Res.. 38. 289-301 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 木下彩栄 ら: "Differential localization of septins in the mouse brain"J.Comp.Neurol.. 428. 223-239 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 秋山暢丈 ら: "Identification of a series of TGF-beta-responsive genes by retrovirus-mediated gene trap screening"Mol.Cell.Biol.. 20. 3266-3273 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 松本貴子 ら: "Transient overexpression of NGFI-A gene suppresses NGF-induced neurite outgrowth in PC12 cells"NeuroReport. 11. 1001-1005 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 秋山暢丈: "Identification of a series of TGF-β-responsive genes by retrovirus-mediated gene-trap screening"Molecular and Cellular Biology. (印刷中).

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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