| Project/Area Number |
11357017
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 展開研究 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Osaka University |
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Principal Investigator |
MORIMOTO Toshifumi Graduate School of Dentistry, Osaka University, Professor, 大学院・歯学研究科, 教授 (20028731)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Tomio Syowa University Faculty of Dentistry, Osaka University, Professor, 歯学部, 教授 (70184760)
IWATA Koichi Graduate School of Dentistry, Osaka University, Associate Professor, 大学院・歯学研究科, 助教授 (60160115)
KANDA Kenro Osaka University, 生理, 主任研究員 (50009635)
YAMADA Yoshiaki Niigata University Faculty of Dentistry, Professor, 大学院・歯学総合研究科, 教授 (80115089)
MASUDA Yuji Graduate School of Dentistry, Osaka University, Assistant Professor, 大学院・歯学研究科, 講師 (20190366)
前田 定秋 大阪大学, 歯学部, 助教授 (00135732)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥34,980,000 (Direct Cost: ¥33,300,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2001: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2000: ¥12,400,000 (Direct Cost: ¥12,400,000)
Fiscal Year 1999: ¥15,300,000 (Direct Cost: ¥15,300,000)
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| Keywords | serotnine / trigeminal motoneuron / mastication / dopamine / basal ganglia / chronic pain / sensitization / opioid / CFA / c-Fos / 線条体 / 摂食行動 / 過敏化 / 三叉神経運動ニューロン / 口腔感覚 / 侵害受容 / 老化 |
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| Research Abstract |
This project was to clarify the role of the nigrostriatal dopaminergic pathway in the sequential progress during mastication.
Some clinical observations and animal experiments have been suggested that the deficencyt of the oral function decrease the activities of brain function inclulding learning and memory. However; the detail of this mechanisrn has not been clarified. In order to understand the relationship between brain and the oral function, we focused on the bioactive materials in brain, in this project.
We obtained the findings in this study as follows ;
1) To know the relationship between the monoamine and the activity trigeminal motor function properties of the hyperpolarizing spike afterpotential and the low-voltage activated Ca^<2+> channel were examined in vitro. It was found serotonine increased the excitability of the trigeminal motoneurons.
2) By using the microdialysis method, the dopamine concectration in the putamen was found to increase significantly during mastication c
… More
ompared with resting period. And the bilateral 6-hydroxydopamine lesions of the nigrostriatal dopaminergic pathway induced the change of the movement pattern during intake behavior. These results suggested that the nigrostriatal dopaminergic pathway might play a role in the sequential progress during the mastication.
3) When the experimental animals were induced chronic pain by making the inflammation in the temporomandibular joint or parotid gland, activities of neurons ln the spinal trigeminal nucleus caudaris were recorded. The data indicated the peripheral and central sensitization occurred, and suggested the opioid might be involved in this sensitization.
4) Using the experimental animals in which masticatory behavior were restricted by giving them only soft food, c-fos expression, in the dorsal horn were examined when inflammation weas induced in hindpaw. C-fos expression was decreased when that experimental animal were activated the masticatory function with hard food and wood stick. These results suggested the possibility that is enhancement of mastication evoked the secretion of the opioid. Less
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