| Project/Area Number |
11440232
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | SAITAMA UNIVERSITY |
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Principal Investigator |
SATO Naoki SAITAMA UNIVERSITY, MOLECULAR BIOLOGY, PROFESSOR, 理学部, 教授 (40154075)
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| Co-Investigator(Kenkyū-buntansha) |
OHTA Niji SAITAMA UNIVERSITY, MOLECULAR BIOLOGY, ASSISTANT PROFESSOR, 理学部, 助手 (60257186)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥15,800,000 (Direct Cost: ¥15,800,000)
Fiscal Year 2000: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 1999: ¥12,900,000 (Direct Cost: ¥12,900,000)
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| Keywords | Plastid nucleoid / RNA polymerase / in vitro transcription / DNA-binding protein / sulfite reductase / HUタンパク質 / ヘパリン / 真核・原核ハイブリッド |
|---|
| Research Abstract |
Plastid nucleoid, which is a functional unit of plastid DNA, is considered as a complex or hybrid of prokaryotic DNA with eukaryotic DNA-binding proteins. Based on this standpoint, we tried to identify the components of plastid nucleoids and to analyze their function in the replication and transcription of plastid DNA. The publication of the complete nucleotide sequence of the genome of Arabidopsis thaliana made us conclude that many of the prokaryotic nucleoid proteins are not present in higher plants. Examples are the HU protein and Dps protein that are ubiquitously present in prokaryotes but these are not present in the chloroplast of A. thaliana.Many of the nucleoid proteins must have been acquired during the evolution of land plants by duplication or modification of the nuclear genes encoding eukaryotic DNA-binding proteins. These results prompted us to put forward a hypothesis on the discontinuous evolution of plastid genomic machinery.
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