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Molecular-approach Diagnosis of Green gases Emission from landfill sites of Municipal Solid Wastes

Research Project

Project/Area Number 11450196
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Civil and environmental engineering
Research InstitutionNagaoka University of Technology

Principal Investigator

HARADA Hideki  Nagaoka University of Technology, Faculty of Engineering, Professor, 工学部, 教授 (70134971)

Co-Investigator(Kenkyū-buntansha) OHASHI Akiyoshi  Nagaoka University of Technology, Faculty of Engineering, Assoc.Professor, 工学部, 助教授 (70169035)
SEKIGUCHI Yuji  Nagaoka University of Technology, Faculty of Engineering, Assis.Professor, 工学部, 助手 (20313570)
ARAKI Nobuo  Nagaoka National College of Technology, Department of Civil Engineering, Assoc.Professor, 環境都市工学科, 助教授 (30193072)
UEMURA Shigeki  Kisarazu National College of Technology, Department of Civil Engineering, Assoc.Professor, 環境都市工学科, 助教授 (60300539)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥14,600,000 (Direct Cost: ¥14,600,000)
Fiscal Year 2000: ¥4,200,000 (Direct Cost: ¥4,200,000)
Fiscal Year 1999: ¥10,400,000 (Direct Cost: ¥10,400,000)
KeywordsLandfill / Phylogenetic analysis / Hybridization / Methane-oxidizing bacteria / Methanogen / Green house gas / FISH / 16S rRNA / 16S rRNA / クローニング
Research Abstract

In the present study, we analyzed 1) initial degradation process of organic organic fraction of municipal solid wastes (OFMSW) in the laboratory-scale vertical column, which simulates landfill site soil layers, 2) structure and diversity of the microbial communities of landfill site soil. Four simulated landfill columns were prepared with different conditions with respect to oxygen and water availability. A significant extent of OFMSW degradation occurred during rather earlier stage of 150 days, corresponding to acid-producing phase. During the following period organic degradation becomes less and less with time elapsed, corresponding to methane-producing phase. Based on these observation, we proposed the way of landfill site operation, so as to 1) enhance aerobic condition at the upper portion of landfill site, and 2) recirculate the leachate liquid. Methane production yield was highest in bottom of the columns (e.g., anaerobic part) while it was lowest in surface of the columns (e.g., aerobic part). Thus result suggested that net methane production in the site should be subtracted by methane oxidizing bacteria. Contribution of the each column parts to global warming was evaluated, based on the gas generation data during the first year, and the result showed that contribution of semi aerobic part was much higher compared with that of aerobic site in which significant amount of carbon dioxide was produced.
This result suggested that net methane production in the landfill sites should be subtracted. by methane oxidizing' bacteria. Contribution of the each column parts to global warming was evaluated, based on the gas generation data during the first year, and the result showed that contribution of semi aerobic part was much higher compared with that of aerobic part in which significant amount of carbon dioxide was produced.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (17 results)

All 2001 2000 Other

All Journal Article (9 results) Publications (8 results)

  • [Journal Article] Population Dynamics of Anaerobic Microbial Consortia in Thermophilic Granular Sludge in Response to Feed Composition Change2001

    • Author(s)
      Shutsubo K., Sinthurat N., Ohashi A., Harada H.
    • Journal Title

      Water Science and Technology 43・1

      Pages: 59-66

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Journal Article] Surface Water pollution in Three Urban Territories of Nepal, India and Bangladesh2001

    • Author(s)
      Sunil K.KARN, Hideki HARADA
    • Journal Title

      Environmental Management Vo1.27

      Pages: 14-14

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Journal Article] Population Dynamics of Anaerobic Microbial Consortia in Thermophilic Granular Sludge in Response to Feed Composition Change2001

    • Author(s)
      Shutsubo K., Sinthurat N., Ohashi A.Harada H.
    • Journal Title

      Water Science and Technology Vo143.1

      Pages: 59-66

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Journal Article] Cultivation and Situ Detection of a Thermophilic Bacterium Capable of Oxidizing Propionate in Syntrophic Association with Hydrogenotrophic Methanogens in a Thermophilic Methanogenic Granular Sludge2000

    • Author(s)
      Imachi, H., Sekiguchi T., Kamagata Y., Ohashi A., Harada H.
    • Journal Title

      Applied and Environmental Microbiology 66

      Pages: 3608-3615

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Journal Article] Quantification of methanogen cell density in anaerobic granular sludge consortia by fluorescence in-situ hybridization2000

    • Author(s)
      T.Tagawa, K.Syutsubo, Y.Sekiguchi, A.Ohashi, H.Harada
    • Journal Title

      Water Science and Technology 42・3-4

      Pages: 77-82

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Journal Article] Syntrophothermus lipocalidus gen.nov., sp.nov., a novel thermophilic, syntrophic, fatty-acid-oxidizing anaerobe which utilizes isobutyrate2000

    • Author(s)
      Y.Sekiguchi, Y.Kamagata, K.Nakamura, A.Ohashi, H.Harada
    • Journal Title

      International Journal of Syst.Evol.Microbiol. 50・2

      Pages: 771-779

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Journal Article] Cultivation and Situ Detection of a Thermophilic Bacterium Capable of Oxidizing Propionate in Syntrophic Association with Hydrogenotrophic Methanogens in a Thermophilic Methanogenic Granular Sludge2000

    • Author(s)
      Imachi, H., Sekiguchi T., Kamagata Y., Ohashi A.Harada H.
    • Journal Title

      Applied and Environmental Microbiology Vo1.60

      Pages: 3608-3615

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Journal Article] Quantification of methanogen cell density in anaerobic granular sludge consortia by fluorescence in-situ hybridization2000

    • Author(s)
      T.Tagawa, K.Syutsubo, Y.Sekiguchi, A.Ohashi H.Harada
    • Journal Title

      Water Science and Technology Vo142.3-4

      Pages: 77-82

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Journal Article] Syntrophothermus lipocalidus gen. nov., sp. nov., a novel thermophilic, syntrophic, fatty-acid-oxidizing anaerobe which utilizes isobutyrate2000

    • Author(s)
      Y.Sekiguchi, Y.Kamagata, K.Nakamura, A.Ohashi, H.Harada
    • Journal Title

      International Journal of Syst.Evol.Microbiol. Vol.50.2

      Pages: 771-779

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shutsubo K.,Sinthurat N.,Ohashi A.& Harada H.: "Population Dynamics of Anaerobic Microbial Consortia in Thermophilic Granular Sludge in Response to Feed Composition Change"Water Science and Technology. 43・1. 59-66 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Imachi,H.,Sekiguchi T.,Kamagata Y.,Ohashi A.and Harada H.: "Cultivation and Situ Detection of a Thermophilic Bacterium Capable of Oxidizing Propionate in Syntrophic Association with Hydrogenotrophic Methanogens in a Thermophilic Methanogenic Granular Sludge"Applied and Environmental Microbiology. 66. 3608-3615 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Tagawa,K.Syutsubo,Y.Sekiguchi,A.Ohashi and H.Harada: "Quantification of methanogen cell density in anaerobic granular sludge consortia by fluorescence in-situ hybridization"Water Science and Technology. 42・3-4. 77-82 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Sekiguchi,Y.Kamagata,K.Nakamura,A.Ohashi & H.Harada: "Syntrophothermus lipocalidus gen.nov., sp.nov., a novel thermophilic, syntrophic, fatty-acid-oxidizing anaerobe which utilizes isobutyrate"International Journal of Syst.Evol.Microbiol.. 50・2. 771-779 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Sekiguchi,Y.Kamagata,K.Nakamura,A.Ohashi & H.Harada: "Syntrophothermus lipocalidus gen. nov., sp. nov., a novel thermophilic, syntrophic, fatty-acid-oxidizing anaerobe which utilizes isobutyrate"International Journal of Systematic and Evolutionary Microbiology. 50(2). 771-779 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 上田久美子、関口勇地、大橋晶良、原田秀樹: "廃棄物埋立土壌におけるメタン生成・酸化に関わる微生物の解析"日本水環境学会年回講演集. 34. 494 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 四蔵茂雄、原田秀樹: "都市廃棄物の嫌気性消化-現状と課題"廃棄物学会誌. 10(3). 241-250 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 大橋晶良、上田久美子、肥田野哲樹、原田秀樹: "FISH法による土壌内メタン生成細菌及びメタン酸化細菌の検出"土木学会第54回年次学術講演会講演概要集. 54. 444-445 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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