| Project/Area Number |
11450317
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物・生体工学
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
ISHIKAWA Haruo Osaka Prefecture University, Graduate School of Engineering, Professor, 大学院・工学研究科, 教授 (00081349)
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| Co-Investigator(Kenkyū-buntansha) |
OGINO Hiroyasu Osaka Prefecture University, Graduate School of Engineering, Lecturer, 大学院・工学研究科, 講師 (80233443)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥14,500,000 (Direct Cost: ¥14,500,000)
Fiscal Year 2001: ¥2,900,000 (Direct Cost: ¥2,900,000)
Fiscal Year 2000: ¥6,700,000 (Direct Cost: ¥6,700,000)
Fiscal Year 1999: ¥4,900,000 (Direct Cost: ¥4,900,000)
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| Keywords | organic solvent-stable enzyme / organic solvent-tolerant microorganism / lipase / protease / nonaqueous bioprocess / タンパク質工学 / Pseudomonas aeruginosa / 酵素精製 |
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| Research Abstract |
Enzymes exhibit high substrate and reaction specificities as catalysts under mild conditions. Therefore, if enzymes are stable in the presence of organic solvents, they can be applied to develop environmentally friendly synthetic processes of fine-chemicals. In this research project, the clarification of the organic solvent-stability of enzymes based on protein engineering and molecular evolution engineering and fundamental research to develop organic solvent-stable enzymes were performed. The examined item in this research is as follows.
1) Growth of organic solvent-tolerant Pseudomonas aeruginosa LST-03 in the presence of an organic solvent and production of organic solvent-stable enzymes
2) Purification and characterization of organic solvent-stable LST-03 lipase
3) Cloning and sequence of the gene of organic solvent-stable LST-03 lipase
4) Cloning and sequence of the lipase genes of Pseudomonas aeruginosa LST-03
5) Purification and characterization of organic solvent-stable PST-01 protease
6) Peptide synthesis catalyzed by PST-01 protease
7) Conformational change of enzymes in the presence of an organic solvent
8) Cloning and sequence of the gene of organic solvent-stable PST-01 protease
9) Role of disulfide bonds of organic solvent-stable PST-01 protease
10) Clarification of the organic solvent-stability of enzymes based on molecular evolution engineering
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