Studies and functions of indolamine N-acetyltransferase in the brain, midgut and reproduction glands in insects

• Project/Area Number: 11460021
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 植物保護
• Research Institution: Kobe University
• Principal Investigator: TAKEDA Makio Kobe University, Graduate School of Science and Technology Professor, 自然科学研究科, 教授 (20171647)
• Project Period (FY): 1999 – 2002
• Project Status: Completed (Fiscal Year 2002)
• Budget Amount: ¥6,100,000 (Direct Cost: ¥6,100,000); Fiscal Year 2002: ¥700,000 (Direct Cost: ¥700,000); Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000); Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000); Fiscal Year 1999: ¥3,200,000 (Direct Cost: ¥3,200,000)
• Keywords: Neurotransmitter / Biological Clock / Indolamine Metabolism / N-acetyltransferase / Midgut / Reproductive Glands / Photoperiodism / Melatonin / インドールアミン / セロトニン / ワモンゴキブリ / 細胞増殖 / 生殖腺成熟 / N-アセチル転移酵素(NAT) / Cyc / 概日振動 / Cycle / Period / Double time / CYC / PER / DBT / CRY

Research Abstract

cDNAs encoding N-acetyltransferase (NAT) were cloned from Priplaneta americana and Antheraea pernyi. Northern analyses and real time PCR analyses showed that transcriptional activity has day / night periodicity. Immunocytochemical investigations suggest that NAT is closely associated with the circadian clock elements in Bombyx mori and A. pernyi. It seems likely that NAT constitutes an important output regulator in the clock system. Also demonstrated are that NAT functions as a switch regulating cell turn-over rate in the midgut and that it regulates the maturation of oocytes in the ovary. One of the NAT-metabolites, melatonin affected the synchronization of circadian rhythm of locomotion in a cricket and fat accumulation in a bug.

Research Products

• [Publications] Takeda, M.: "Photoperiodic system in physiological reality"Entomological Science. Vol.2 No.4. 567-574 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tohno, Y.: "Two types of diapause development in the Chinese oak silkmoth, Antheraea pernyi"International Journal of Wild Silkmoth & Silk. Vol.5. 31-39 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ichihara, N.: "Characterization and purification of polymorphic arylalkylamine N-acetyltransferase from the American cockroach, Periplaneta americana"Insect Biochemistry and Molecular Biology. Vol.32. 15-22 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yamano, H.: "Melatonin drinking water influences a circadian rhythm of locomotor activity in the house cricket, Acheta domesticus"Journal of Insect Physiology. Vol.47. 943-949 (2001)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Matsumoto, M.: "Changes in brain monoamine contents in diapause pupae of Antheraea pernyi when activated under long-day and by chilling"Journal of Insect Physiology. Vol.48. 765-771 (2002)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Asano, H.: "Multiple forms of arylalkylamine N-acetyltransferase (NAT) from cockroach female colleterial glands and activity changes along oocyte maturation"Comparative Biochemistry and Physiology A. Vol. 134. 795-803 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Niva, C.: "Effect of photoperiod and melatonin on nymphal development, polyphenism and reuroduction in Hyalompmha halys (Heteroptera ; Pentatomidae)"Zoological Science. (in press).
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 竹田 真木生: "環境昆虫学"東大出版. 568 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 竹田 真木生: "昆虫学大事典"朝倉書店. 1220 (2003)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Takeda, M.: "Photoperiodic system in physiological reality"Entomological Science. Vol. 2, No. 4. 567-574 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Tohno, Y.: "Two types of diapause development in the Chinese oak silkmoth, Antheraea pernyi"International Journal of Wild Silkmoth & Silk. Vol. 5. 31-39 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ichihara, N.: "Characterization and purification of polymorphic arylalkylamine N-acetyltransferase from the American cockroach, Periplaneta americana"Insect Biochemistry and Molecular Biology. Vol. 32. 15-22 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yamano, H.: "Melatonin drinking water influences a circadian rhythm of locomotor activity in the house cricket, Acheta domesticus"Journal of Insect Physiology. Vol. 47. 943-949 (2001)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Matsumoto, M.: "Changes in brain monoamine contents in diapause pupae of Antheraea pernyi when activated under long-day and by chilling"Journal of Insect Physiology. Vol. 48. 765-771 (2002)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Asano, H.: "Multiple forms of arylalkylamine N-acetyltransferase (NAT) from cockroach female colleterial glands and activity changes along oocyte maturation"Comparative Biochemistry and Physiology A. Vol. 134. 795-803 (2003)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Niva, C.: "Effect of photoperiod and melatonin on nymphal development, polyphenism and reproduction in Hyalomorpha halys (Heteroptera; Pentatomidae)"Zoological Science. (in press).
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Makio Takeda: "Photoperiodic system in physiological reality"Entomological Science. Vol.2 No.4. 567-574 (1999)
• [Publications] Naoyuki Ichihara: "Characterization and purification of polymorphic arylalkylamine N-acetyltransferase from the American cockroach, Periplaneta americana"Insect Biochemistry and Molecular Biology. Vol.32. 15-22 (2000)
• [Publications] Hirotsugu Yamano: "Melatonin drinking water influences a circadian rhythm of locomotor activity in the house cricket, Acheta domesticus"Journal of Insect Physiology. Vol.47. 943-949 (2001)
• [Publications] Masayuki Matsumoto: "Changes in brain monoamine contents in diapause pupae of Antheraea pernyi when activated under long-day and by chilling"Journal of Insect Physiology. Vol.48. 765-771 (2002)
• [Publications] Hironobu Asano: Comparative Biochemistry and Physiology. (in press).
• [Publications] 三橋 淳(編): "昆虫学大事典(5.10.2:神経伝達物質,竹田真木生)"朝倉書店. 1220 (2003)
• [Publications] Yamano, H., Watari, Y., Arai T., Takeda: "MMelatonin in drinking water influences a circadian rhythm in locomotor activity in the house cricket, Acheta domesticus"J.Insect Physiol.. 47. 943-949 (2001)
• [Publications] Uwo, M., Tei, K., Park, Takeda M.: "Replacement of midgut epithelium in the greater wax moth, Galleria mellonela during larval-pupal moult"Cell Tiss. Res.. (in press).
• [Publications] Tufail, M., Hatakeyama M., Takeda M.: "Molecular Evidence for two vitellogenin genes and processing of vitellogenins in the American cockroach, Periplaneta americana"Arch. Insect Biochem. Physiol.. 48. 72-80 (2001)
• [Publications] Youhei Tohno, Makio Takeda: "Sex-specific circadian dynamics of cyclic nucleotides in the cerebral ganglia of a univoltine strain of Antheraea pernyi during post-diapause development"Internat. J. Wild Silkmoths & Silk. 6. 17-24 (2001)
• [Publications] Loeb, M.J., Martin, P.A.W., Narang, N., Hakim, R.S., Goto, S., Takeda, M.: "Control of life, death and differentiation in cultured midgut cells of the lepidopteran, Heliothis virescens"In Vitro Cell. Dev. Biol.. 37. 348-352 (2001)
• [Publications] Ichihara, N., M.Okada, M.Takeda.: "Purification of polymorphic arylalkylamine N-acetyltransferase from the American cockroach, Periplaneta americana"InsectBiochemistry Molec. Biol.. 32. 15-22 (2001)
• [Publications] Tohno,Youhei: "Two types of diapause development in the Chinese oak silkmoth,Antheraea perinyi."International Journal of Wild Silkmoths and Silk. 5. 31-39 (2000)
• [Publications] Tufail,Mohammad: "Cloning of vitellogenin cDNA of the American cockroach,Periplaneta Americana and its structural and expressin analyses"Archives of Insect Biochemistry and Physiology. 45. 37-46 (2000)
• [Publications] Sattelle,D.B.: "Immunocytochemical localization of putative γ-aminobutyric acid receptor subunits in the head ganglia of Periplaneta americana using an anti-RDL C-terminal antibody."Neuroscience Letter. 289. 197-200 (2000)
• [Publications] Choi,K.-H.: "Developmental and life history characteristics of the mushroom fly,Coboldia fuscipes."Applied Entomology and Zoology. 35. 495-504 (2000)
• [Publications] Loeb,M.J.: "Apoptosis in cultured midgut cells from Heliothis virescence larvae exposed to various conditions"Archives of Insect Biochemistry and Physiology. 45. 12-23 (2000)
• [Publications] Yamano,H.: "Melatonin in drinking water influences a circadian rhythm in locomotor activity in the house cricket,Acheta domesticus"Journal of Insect Physiology (in press). (2001)
• [Publications] Naeemullah,M.: "Relationship of cold tolerance to developmental determination in the Indian meal moth Plodia interpunctella(Lepidoprera:Phycitidae)"Appl.Entomol.Zool.. 34. 267-276 (1999)
• [Publications] Takeda,M.: "Photoperiodism in physiological reality"Entomol.Sci.. 567-574 (1999)