• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of novel insecticidal crystal protein genes from B.thuringiensis and application of biological insecticide

Research Project

Project/Area Number 11460026
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field 蚕糸・昆虫利用学
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

ASANO Shin-ichiro (2000)  Hokkaido Univ.Grad.School of Agr., Asso. Prof., 大学院・農学研究科, 助教授 (60222585)

飯塚 敏彦 (1999)  北海道大学, 大学院・農学研究科, 教授 (50001441)

Co-Investigator(Kenkyū-buntansha) SAHARA Ken  Hokkaido Univ.Grad.School of Agr., Inst.., 大学院・農学研究科, 助手 (30241368)
浅野 眞一郎  北海道大学, 大学院・農学研究科, 助教授 (60222585)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥11,200,000 (Direct Cost: ¥11,200,000)
Fiscal Year 2000: ¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 1999: ¥7,700,000 (Direct Cost: ¥7,700,000)
KeywordsBacillus thuringiensis / insecticidal crystal protein / cry gene / Scarab-cidal / Nematoda-cidal / Lepidoptera-cidal / コガネムシ / 殺線虫活性cry遺伝子 / cryB28遺伝子 / cry 1遺伝子 / ドウガネブイブイ / 結晶タンパク質遺伝子 / cry8遺伝子
Research Abstract

1. Screening of a scarab-cidal B.thuringiensis (1999)
In order to find of a scarab-cidal B.thuringiensis, we were screening our B.thuringiensis stocks.
2. Analysis of the scarab-cidal B.thuringiensis strain (gallariae BBT2-8)(2000)
We found the scarab-cidal Bt.BBT2-8, then we cloned the crystal protein genes (cryB28 gene) have a scarab-cidal activity. This cryB28 gene was expressed in E.coli and acrystariferous B.thuringiensis strain (BT51). The expression products had a highly activity against scarabe.
3. Screening of a nematoda-cidal B.thuringiensis (1999-2000)
In order to find of a nematoda-cidal B.thuringiensis, we were screening our B.thuringiensis stocks by using PCR.We could not find nematodacidal B.thuringiensis strains.
4. Screening of novel lepidopteran-cidal B.thuringiensis (1999)
In order to find of novel lepidopteran-cidal B.thuringiensis, we were screening our B.thuringiensis stocks. We picked up the novel lepidopteran acitive Bt strain (wuhanensis), then we cloned the novel cry1 gene (cry1Db1) from wuhanensis have insecticidal activities against Spodoptera litura, Plutella xylostella and Bombyx mori.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Toshihiko Iizuka: "Historical Review on Bacillus thuringiensis Researchs"Biotechnology of Bacillus thuringiensis. Vol.3. 3-5 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yulia Pujiastuti: "Insecticidal crystal proteins isolated from Bacillus thuringiensis subsp.wuhanensis towards lepidopteran insects"Biotechnology of Bacillus thuringiensis. Vol.3. 53-57 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yulia Pujiastuti: "Toxicity of Bacillus thuringiensis subsp.wuhanensis crystal protein to Bombyx mori and Spodoptera litura"J.Sericultural Science Japan. 68巻3号. 195-199 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 菊田治典: "屋久島土壌から分離されたB.thuringiensis I.H-serotypeのフローラ"日本蚕糸学雑誌. 68巻3号. 217-224 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 菊田治典: "屋久島土壌から分離されたB.thuringiensis II.殺虫活性とcry遺伝子"日本蚕糸学雑誌. 68巻3号. 225-236 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Toshihiko Iizuka: "Historical Review on Bacillus thuringiensis Researchs"Biotechnology of Bacillus thuringiensis. Vol.3. 3-5 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yulia Pujiastuti, Shin-ichiro Asano, Ken Sahara, Hisanori Bando, Toshihiko Iizuka: "Insecticidal crystal proteins isolated from Bacillus thuringiensis subsp. wuhanensis towards lepidopteran insects"Biotechnology of Bacillus thuringiensis. Vol.3. 53-57 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yulia Pujiastuti, Shin-ichiro Asano, Ken Sahara, Hisanori Bando, Toshihiko Iizuka: "Toxicity of Bacillus thuringiensis subsp. wuhanensis crystal protein to Bombyx mori and Spodoptera litura"J.Sericultural Science Japan. Vol.68-3. 195-199 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Harunori Kikuta, Manabu Kuroiwa, Ryuichiro Takagi, Toshihiko Iizuka: "Sutudy on the isolation and identification of Bacillus thuringiensis in soil sample from Yakushima Island (I) Serological identification and flora"J.Sericultural Science Japan. Vol.68-3. 217-224 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Harunori Kikuta, Manabu Kuroiwa, Ryuichiro Takagi, Toshihiko Iizuka: "Sutudy on the isolation and identification of Bacillus thuringiensis in soil sample from Yakushima Island (II) Insecticidal activity and the identification of cry genes"J.Sericultural Science Japan. Vol.68-3. 225-236 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 浅野眞一郎: "B.thuringiensis serovar galleriae BBT2-8株の性状"東北蚕糸研究報告. 25号(印刷中). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 石田葉子: "オオモンシロチョウにおけるBacillus thuringiensis subsp. thuringiensis HD-2株のトキシンレセプターの検索"東北蚕糸研究報告. 25号(印刷中). (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 飯塚 敏彦: "ドウガネブイブイに活性を有する B. thuringiensis serovar galleriae BBT2-8株のcry遺伝子"日本蚕糸学会東北支部 秋期発表会 東北蚕糸研究報告. 25号(発表予定). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 飯塚 敏彦: "Screening of novel cry genes from Bacillus thuringiensis and analysis of their nucleotide sequences"Proceedings of an International Symposium on the Proteins for Insect Pest Control. (印刷中). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shin-ichiro Asano: "Screening for scarabecidal crystal proteins from Bacillus thuringiensis isolates"Biotechnology of Bacillus thuringiensis. vol.3. 72 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toshihiko Iizuka: "Biotechnology of Bacillus thuringiensis Vol. 3"Science Press. 246 (1999)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi