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Study on karyotype and genome size of entomopathogenic microsporidians

Research Project

Project/Area Number 11460027
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field 蚕糸・昆虫利用学
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

KAWARABATA Takeshi  Faculty of Agriculture, KYUSHU UNIVERSITY Prof., 大学院・農学研究院, 教授 (60038221)

Co-Investigator(Kenkyū-buntansha) AOKI-YASUNAGA Chisa  Faculty of Agriculture, KYUSHU UNIVERSITY Ass.Prof., 大学院・農学研究院, 助教授 (20264103)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥12,100,000 (Direct Cost: ¥12,100,000)
Fiscal Year 2000: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1999: ¥10,200,000 (Direct Cost: ¥10,200,000)
Keywordsentomopathogenic microsporidians / cloning / n=13 / alkali EDTA solution / pulse-field gel electrophoresis / 微胞子虫胞子 / 微胞子虫クローン / シグナル強度 / 多重感染 / 樹枝状染色体 / シゾント / ギームザ染色 / 半数体
Research Abstract

The identification of microsporidian parasites has been based on morphological characters observed with light and electron microscopy. Pulsed-field gel electrophoresis has recently provided an alternative for not only the investigation of chromosomes in small single-celled organisms but also the identification of microsporidians. On the other hand, microsporidians used in those studies were not cloned ones. Mixed infection of microsporidians has been known from the diseased silkworm larvae. In this study, the interaction of the parasites in insect hosts and insect cell cultures under mixed infection was investigated first and estimate of the karyotype of the cloned microsporidians was made by light microscopy. Then, we presented data from pulsed-field gel electrophoresis of cloned microsporidians and the intra- and inter-specific variations of entomophathogenic microsporidians was discussed.
Both in the silkworm and in the Spodoptera frugiperda SF21AEII cell culture, Nosema bombycis NIS … More 001 dominated on the other microsporidians, Nosema sp. NIS M11 and Vairimorha sp. NIS M12, when it was involved in the mixed infections, However, the dominant did not eliminate the minor ones by competition and microsporidians could co-exist in the same host. It was, therefore, strongly suggested that cloning of microsporidians would be important when the characterization and identification of the parasites were discussed.
Several clones of those microsporidians in insect cell cultures were established by a limiting dilution method and attempt to estimate the number of chromosomes for the vegetative cells of cloned microsporidians was made by an Axioplan 2 universal microscope. Thirteen chromosomes were visualizied for the microsporidian, Vairimorpha sp. NIS M12. In genomic DNA analysis of microsporidia, it was clarified that alkali EDTA solution treatment of spore was needed for genome extraction from Brazilian isolates. The banding patterns were different among the species of microsporidia in pulse-field gel electrophoresis, suggesting that this method useful tool for identification of microsporidia. Established protocol in this report could apply for all isolates used, and this restult demnonstrated the importance of cloning. Less

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] 青木智佐 ら: "昆虫病原性微胞子虫類の胞子サイズの新測定法"九州蚕糸. 30. 21 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 橋口美保子 ら: "クローニングしたブラジル産微胞子虫の生物学的性状(2)大型胞子産生株について"九州蚕糸. 30. 22 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 寺元理恵 ら: "ブラジルの養蚕農家で発生した微胞子虫病と病原(3)未同定微胞子虫について"九州蚕糸. 30. 23 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 鄭祥明 ら: "中国広東家蚕微粒子胞子在Antheraea eucalypti細胞系的感染増殖"蚕業科学. 25. 170-174

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 寺元理恵 ら: "ブラジルの養蚕農家で発生した微胞子虫病と病原(4)2種類の胞子を産生する微胞子虫について"第56回日本蚕糸学会九州支部研究発表会並びに学術講演会. 1. 21 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 橋口美保子 ら: "昆虫病原性微胞子虫TB-2M-H1株のoctospore形成誘導"第56回日本蚕糸学会九州支部研究発表会並びに学術講演会. 1. 25 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hashiguchi,M. et al.: "A multiple infection of entomopathogenic microsporidians in Bombyx mori."XXXVth Annual Meeting of the Society for Invertebrate Pathology. 33. 51 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Teramoto,R. et al.: "Does pebrine exist in Brazilian silkworm farms?"XXXVth Annual Meeting of the Society for Invertebrate Pathology. 33. 93 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yasunaga-Aoki,C. et al: "Establishment of a new cell line having high phagocytic ability from hemocytes of the beet armyworm, Spodoptera exigua."XXXVth Annual Meeting of the Society for Invertebrate Pathology. 33. 101 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yasunaga-Aoki, C.et al.: "A new method for measuring microsporidian spore size."Proc.Sericult.Sci.Kyushu. 30. 21 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hashiguchi, M.et al.: "Studies of cloned microsporidium isolated from Brazilian silkworm farms (2) A microsporidian producing large spores in Bombyx mori Bmx cell line."Proc.Sericult.Sci.Kyushu. 30. 22 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Teramoto, R.et al.: "Microsporidian disease and pathogen from silkraising farmers in Brazil (3) Unidentified microsporidia."Proc.Sericult.Sci.Kyushu. 30. 23 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Xiangming, Z.et al.: "Infection and Multiplication of Nosema bombycis from silkworm of Guangdong, China in Antheraea eucalypti cell line."Canye Kecue Acta Sericologica sinica. 25. 170-174 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Teramoto, R.et al.: "Microsporidian disease and pathogens from silkraising farms in Brazil (4) Microsporidians producing octospores."Bulletin of Sericul.Sci.of Kyushu. 1. 21 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hashiguchi, M.et al.: "Octospore production of a microsporidium, TB-2M-H1 isolate, in vivo and in vitro."Bulletin of Sericul.Sci.of Kyushu. 1. 25 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hashiguchi, M.et al.: "A multiple infection of entomopathogenic microsporidians in Bombyx mori."XXXVth Annual Meeting of the Society for Invertebrate Pathology. 33. 51 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Teramoto, R.et al.: "Does pebrine exist in Brazilian silkworm farms?"XXXVth Annual Meeting of the Society for Invertebrate Pathology. 33. 93 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yasunaga-Aoki, C.et al.: "Establishment of a new cell line having high phagocytic ability from hemocytes of the beet armyworm, Spodoptera exigua."XXXVth Annual Meeting of the Society for Invertebrate Pathology. 33. 101 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 寺元理恵,青木智佐,橋口美保子,河原畑勇,富丸英博: "ブラジルの養蚕農家で発生した微胞子虫病と病原(4)2種類の胞子を産生する微胞子虫について."第56回日本蚕糸学会九州支部研究発表会並びに学術講演会. 1. 21 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 橋口美保子,青木智佐,寺元理恵,河原畑勇,富丸英博: "昆虫病原性微胞子虫TB-2M-H1株のoctospore形成誘導"第56回日本蚕糸学会九州支部研究発表会並びに学術講演会. 1. 25 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hashiguchi,M.,R.Teramoto,C.Yasunage-Aoki,T.Kawarabata and H.Tomimaru.: "A multiple infection of entomopathogenic microsporidians in Bombyx mori."XXXVth Annual Meeting of the Society for Invertebrate Pathology. 33. 51 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Teramoto,R.,M.Hashiguchi,C.Yasunage-Aoki,T.Kawarabata and H.Tomimaru.: "Does pebrine exist in Brazilian silkworm farms?"XXXVth Annual Meeting of the Society for Invertebrate Pathology. 33. 93 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yasunaga-Aoki,C.,T.Kawarabata,K.Iiyama and S.Imanishi: "Establishment of a new cell line having high phagocytic ability from hemocytes of the beet armyworm, Spodoptera exigua."XXXVth Annual Meeting of the Society for Invertebrate Pathology. 33. 101 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 青木智佐,橋口 美保子,寺元 理恵,河原畑 勇,早坂 昭二: "昆虫病原性微胞子虫類の胞子サイズの新測定法"九州蚕糸. 30. 21 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 橋口 美保子,青木 智佐,寺元 理恵,河原畑 勇,富丸 英博: "クローニングしたブラジル産微胞子虫の生物学的性状 (2)大型胞子産生株について"九州蚕糸. 30. 22 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 寺元 理恵,橋口 美保子,青木 智佐,寺元 理恵,河原畑 勇,富丸 英博: "ブラジルの養蚕農家で発生した微胞子虫病と病原 (3)未同定微胞子虫について"九州蚕糸. 30. 23 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 鄭 祥明,安永智佐,河原畑 勇: "中国広東家蚕粒子胞子在Antheraea eucalypti細胞系的感染増殖"蚕業科学. 25. 170-174 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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