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Bioactive peptides acting on animals and plants

Research Project

Project/Area Number 11460045
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field 応用微生物学・応用生物化学
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

YOSHIKAWA Masaaki  Kyoto University, Research Institute for Food Science, Professor, 食糧科学研究所, 教授 (50026572)

Co-Investigator(Kenkyū-buntansha) YOKOTA Hiromi  Shizuoka University, Faculty of Agriculture, Professor, 農学部, 教授 (50115458)
FUJISAWA Toshitaka  National Institute of Genetics, Associate Professor, 個体遺伝研究部門, 助教授 (60000262)
TAKENAKA Yasuyuki  Kyoto University, Research Institute for Food Science, Instructor, 食糧科学研究所, 助手 (20273518)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥14,200,000 (Direct Cost: ¥14,200,000)
Fiscal Year 2000: ¥5,600,000 (Direct Cost: ¥5,600,000)
Fiscal Year 1999: ¥8,600,000 (Direct Cost: ¥8,600,000)
Keywordssoybean / soymetide / β-conglycinin / Brassica campestris / pollen tube extension / root extension / angiotensin / fMLP / 花粉管 / 成長促進 / 免疫促進 / ファゴサイトーシス / 血圧上昇
Research Abstract

Ala-Met-Lys-Pro-Trp, which was isolated as a pollen tube-extending peptide from casein digest exhibited weak hypertensive activity after iv. administration in rats. While hypertensive activity of the peptide was blocked by an angiotensin AT1 antagonist, the pollen tube-extending activity was not affected by the antagonist.
Soymetide which was isolated as an immunostimulating peptide from soybean digest acts through fMLP receptor. Soymetide-4 (Met-Ile-Thr-Leu), the minimum peptide to show immunostimulating activity promoted extension of tea pollen tube. It also stimulated extension of Brassica campestris root at 10^<-7>M.The activity seems to be not a mere nutritional effect since the extension was inhibited by the peptide above 10^<-4>M.Soymetide-4 exhibited the highest activity both for stimulation of pollen tube and root exyension among the soymetide fragment peptides of various length. Ala-Met-Lys-Pro-Trp derived from casein as descibed above also stimulated root extension. Although fMLP weakly stimulated pollen tube extension, it was inactive for root.
Methioninyl residue in soymetide-4 was essential for both immunostimulation and extension of pollen tube and root. However, the peptide showing the maximum activity was different for these three activities among soymetide-4 derivatives ; the best peptide for immunostimulation, pollen tube extension and root extension was MIWL, MRGG and M (O) TL, respectively. These results suggest that receptors mediating these three activities are different each other though elevation of intracelullar calcium level is the common post-receptor event following them.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] M.Yoshikawa: "Bioactive peptides derived from food proteins preventing lifestyle-related diseases."Bio Factors. 12. 143-146 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 吉川正明: "タンパク質から派生する生理活性ペプチド-レセプターリガンドを中心に-"Peptide Newsletter Japan. 37. 2-3 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takahashi,M.and Yoshikawa,M.: "Peptide Science 2000"Protein Research Foundation, Osaka. 458 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tsuruki,T.and Yoshikawa,M.: "Peptide Science 2000"Protein Research Foundation, Osaka. 458 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshikawa, M., Fujita, H, Matoba, N., Takenaka, Y., Yamomoto, T., Yamauchi, R., Tsuruki, T.and Takahata, K.: "Bioactive peptides derived from food proteins preventing lifestyle-related diseases."BioFactors. 12(1-4). 143-146 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshikawa, M.: "Bioactive peptides derived from food proteins, -ligands for receptors-."Peptide Newsletter Japan. 37. 2-3 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takahashi, M., Matsukawa T.and Yoshikawa, M.: "A soy bean-derived peptide extending pollen tube and roots."Peptide Science 2000. 393-396 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tsuruki, T., Takahata, K.and Yoshikawa, M.: "fMLP agonists as anti-alopecia peptides."Peptide Science 2000. 221-224 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] M.Yoshikawa: "Bioactive peptides derived from food proteins preventing lifestyle-related diseases."BioFactors. 12. 143-146 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 吉川正明: "タンパク質から派生する生理活性ペプチド-レセプターリガンドを中心に-"Peptide Newsletter Japan. 37. 2-3 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] T.Tsuruki: "Peptide Science 2000"Protein Research Foundation, Osaka. 458 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] M.Takahashi: "Peptide Science 2000"Protein Research Foundation, Osaka. 458 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fukunaga H.,Takahashi M.,Kaneto H.and Yoshikawa M.: "Effect of Tyr-MIF-1 on stress-induced analgesia and the blockade of devopment of morphine tolerance by stress in mice"Jpn. J. Pharmacol.. 79・(2). 231-235 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Matoba N.,Usui H.,Fujita H.and Yoshikawa M.: "A novel antihypertensive peptide derived from ovalbumin induces nitric oxidemediated vasorelaxation in an isolated SHR mesenteric artery"FEBS Lett.. 452・(3). 181-184 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yunden J.and Yoshikawa M.: "Enzymatic release of neocasomorphion and β-casomorphin from bovine β-casein"Peptides. 20・(4). 957-962 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Fujita H.and Yoshikawa M.: "LKPNM: a prodrug type ACE-inhibitory peptide derived from fish protein"Immunopharmacology. 44・(1). 123-127 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 吉川正明: "植物タンパク質由来ペプチドの生理作用"ブレインテクノニュース. 75. 5-7 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 吉川正明: "意外な起源の生理活性ペプチド"環境と健康. 12. 353-370 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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