| Project/Area Number |
11460049
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| Research Category |
Grant-in-Aid for Scientific Research (B).
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
応用微生物学・応用生物化学
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| Research Institution | Osaka Prefecture University |
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Principal Investigator |
ARAI Motoo Graduate School of Agricultur and Biological Sciences, Osaka Prefecture University Professor, 農学生命科学研究科, 教授 (80081537)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAGUCHI Takashi The sama as above, Associate Professor, 農学生命科学研究科, 助教授 (70195056)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥9,200,000 (Direct Cost: ¥9,200,000)
Fiscal Year 2000: ¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 1999: ¥6,500,000 (Direct Cost: ¥6,500,000)
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| Keywords | cellulose / cellulase / gene / cloning / expression / セルロース / セルラーゼ / キシラナーゼ / マンノシダーゼ / 遺伝子 / 糸状菌 / バイオマス |
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| Research Abstract |
For utilization of biomass cellulosic substances are important because they are dominant organic substances on the earth. Cellulases consist of complex system and they hydrolyze cellulosic substances synergistically. We isolated Aspergillus aculeatus which shows synergism with Trichoderma cellulase. When the cellulase from Aspergillus aculeatus was mixed with Trichoderma cellulase, enzyme showed potent synergisty and hydrolyzed completely cellulosic substances. The research elucidated essential cellulase components. The enzyme genes were cloned and then these genes were introduced into yeast cells. Finally, we can expected the yeast which produced ethanol directly from cellulose.
The genes of 1 β-glucosidase, 2 Avicelases, 2 CM-cellulases, β-mannosidase, 1 xylanase were cloned. These genes were expressed in yeast cells. β-mannosidase gene was introduced into Aspergillus. The enzyme production improved in fungal cells.
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