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Studies on pollen coat proteins involved in pollen-stigma interactions in Brassica sp.

Research Project

Project/Area Number 11460056
Research Category

Grant-in-Aid for Scientific Research (B).

Allocation TypeSingle-year Grants
Section一般
Research Field Bioproduction chemistry/Bioorganic chemistry
Research InstitutionNara Institute of Science and Technology

Principal Investigator

TAKAYAMA Seiji  Nara Institute of Science and Technology, Graduate School of Biological Sciences, Associate Professor, バイオサイエンス研究科, 助教授 (70273836)

Co-Investigator(Kenkyū-buntansha) IWANO Megumi  Nara Institute of Science and Technology, Graduate School of Biological Sciences, Technical Staff, バイオサイエンス研究科, 教務職員 (50160130)
SHIBA Hiroshi  Nara Institute of Science and Technology, Graduate School of Biological Sciences, Research Associate, バイオサイエンス研究科, 助手 (20294283)
ISOGAI Akira  Nara Institute of Science and Technology, Graduate School of Biological Sciences, Professor, バイオサイエンス研究科, 教授 (20011992)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥14,900,000 (Direct Cost: ¥14,900,000)
Fiscal Year 2000: ¥6,900,000 (Direct Cost: ¥6,900,000)
Fiscal Year 1999: ¥8,000,000 (Direct Cost: ¥8,000,000)
Keywordsself-incompatibility / pollen / Brassica / PCP / SP11 / SLR1-BP / primary incompatibility / adhesion / 花粉表層蛋白質 / SLP1-BP / 種間認識反応 / 自家不和合成 / SLG / SRK / 花粉側因子
Research Abstract

Plant sexual reproduction depends on highly specific interactions between pollen and pistil. Pistils can recognize to select from the genetically diverse range of pollen arriving on the stigma so that only appropriate pollen effects fertilization. These systems include barriers to cross-species fertilization and intraspecific barriers such as self-incompatibility (SI) that prevent self-fertilization.
In Brassica, stigmaticaly expressed members of a protein family, SLG family, are expected to play central roles in these recognition systems. SRK (S-receptor kinase) and SLG (S-locus glycoprotein), are shown to be involved in the recognition of self/non-self pollen in SI.SLR1 is suggested to be involved in the initial stages of pollen-stigma adhesion. Although these familial proteins are expected to interact with some components on pollen grains, the molecular nature of these components remained elusive.
In this study, we searched for these interacting molecules in PCP (pollen coat protein) family of proteins and succeeded to identify two key component named SP11 and SLR1-BP.SP11, which is encoded in the self-incompatibility (S) locus and belongs to a novel class of PCP family, is tumed out to be the pollen determinant of SI.We presented experimental evidence showing that SP11 is working as a self/non-self recognition marker which is perceived by SRK on stigmatic papillar cells. SLR1-BP, which is a new member of PCP class A proteins, is interacting with SLR1 with high affinity. Although the biological signficance of SLR1-BP is as yet to be exprimentally demonstrated, the interaction between SLR1 and SLR1 -BP is expected to be involved in the pollen-stigma adhesion process. These two pollen components identified in this study should be key molecules in understanding the pollen-stigma recognition process.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (37 results)

All Other

All Publications (37 results)

  • [Publications] Suzuki,Go: "Genomic organization of the S locus : Identification and characterization of genes in SLG/SRK region of S9 haplotype of Brassica campestris (syn.rapa)."Genetics. 153. 391-400 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Iwano,Megumi: "X-ray microanalysis of papillar cells and pollen grains in the pollination process in Brassica using variable-pressure scanning electron microscope."J.Electron Microsc.. 48. 101-109 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Watanabe,Masao: "Two anther-expressed genes downstream of SLG9 : Identification of a novel S-linked gene specifically expressed in anthers at the uninucleate stage of Brassica campestris (syn.rapa) L."Sex.Plant Reprod.. 12. 127-134 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takasaki,Tsuyoshi: "Introduction of SLG (S locus glycoprotein) alters the phenotype of endogenous S haplotype, but confers no new S haplotype specificity in Brassica rapa L."Plant Mol.Biol.. 40. 659-668 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Watanabe,Masao: "Molecular biology of self-incompatibility in Brassica species."Plant Biotechnol.. 16. 263-272 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takasaki,Takeshi: "The S receptor kinase determines self-incompatibility in Brassica stigma."Nature. 403. 913-916 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takayama,Seiji: "The pollen determinant of self-incompatibility in Brassica campestris."Proc.Natl.Acad.Sci.USA. 97. 1920-1925 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takayama,Seiji: "Isolation and characterization of pollen coat proteins of Brassica campestris that interact with S-locus related glycoprotein 1 involved in pollen-stigma adhesion."Proc.Natl.Acad.Sci.USA. 97. 3765-3770 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Watanabe,Masao: "Highly divergent sequences of the pollen self-incompatibility (S) gene in class-I haplotypes of Brassica campestris (syn.rapa) L."FEBS Lett.. 473. 139-144 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Watanabe,Masao: "Genomic organization of the SLG/SRK region of the S-locus in Brassica species."Annal.Bot.. 85. 155-161 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shiba,Hiroshi: "Alteration of the self-incompatibility phenotype in Brassica by transformation of the antisense SLG gene."Biosci.Biotech.Biochem.. 64. 1016-1024 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shiba,Hiroshi: "A pollen coat protein, SP11/SCR, determines the pollen specificity in the self-incompatibility of Brassica sp."Plant Physiol.. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Suzuki, Go et al.: "Genomic organization of the S locus : Identification and characterization of genes in SLG/SRK region on S9 haplotype of Brassica campestris (syn.rapa)."Genetics.. 153. 391-400 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Iwano, Megumi et al.: "X-ray microanalysis of papillar cells and pollen grains in the pollination process in Brassica using variable-pressure scanning electron microscope."J.Electron Microse.. 48. 101-109 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Watanabe, Masao et al.: "Two anther-expressed genes downstream of SLG9 : Identification of a novel S-linked gene specifically expressed in anthers at the uninucleate stage of Brassica campestris (syn.rapa) L."Sex.Plant Reprod.. 12. 127-134 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takasaki, Tsuyoshi et al.: "Introduction SLG (S locus glycoprotein) alters the phenotype of endogenous S haplotype, but confers no new S haplotype specificity in Brassica rapa L."Plant Mol.Biol.. 40. 659-668 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Watanabe, Masao et al.: "Molecular biology of self-incompatibility in Brassica species."Plant Biotechnol.. 16. 263-272 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takasaki, Takeshi et al.: "The S receptor kinase determines self-incompatibility in Brassica stigma."Nature. 403. 913-916 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takayama, Seiji et al.: "The pollen determinant of self-incompatibility in Brassica campestris."Proc.Natl.Acad.Sci.USA. 97. 1920-1925 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takayama, Seiji et al.: "Isolation and characterization of pollen coat proteins of Brassica campestris that interact with S-locus related glycoprotein 1 involved in pollen-stigma adhesion."Proc.Natl.Acad.Sci.USA. 97. 3765-3770 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Watanabe, Masao et al.: "Highly divergent sequences of the pollen self-incompatibility (S) gene in class-I haplotypes of Brassica campestris (syn.rapa) L."FEBS Lett.. 473. 139-144 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Watanabe, Masao et al.: "Genomic organization of the SLG/SRK region of the S-locus in Brassica species."Annal.Bot.. 85. 155-161 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shiba, Hiroshi et al.: "Alteration of the self-incompatibility phenotype in Brassica by transformation of the antisense SLG gene."Biosci.Biotech.Biochem.. 64. 1016-1024 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shiba, Hiroshi et al.: "A pollen coat protein, SP11/SCR, determines the pollen specificity in the self-incompatibility of Brassica sp."Plant Physiol.. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Seiji Takayama: "Isolation and characterization of pollen coat proteins of Brassica campestris that interact with S-locus related glycoprotein 1 involved in pollen-stigma adhesion."Proc.Natl.Acad.Sci.USA. 97. 3765-3770 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Masao Watanabe: "Genomic organization of the SLG/SRK region of the S-locus in Brassica species."Annal.Bot.. 85. 155-161 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Masao Watanabe: "Highly divergent sequences of the pollen self-incompatibility (S) gene in class-I haplotypes of Brassica compestris (syn.rapa) L."FEBS Lett.. 473. 139-144 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hiroshi Shiba: "Alteration of the self-incompatibility phenotype in Brassica by transformation of the antisense SLG gene."Biosci.Biotech.Biochem.. 64. 1016-1024 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tsuyoshi Takasaki: "Introduction of SLG (S locus glycoprotein) alters the phenotype of endogenous S haplotype, but confers no new S haplotype specificity in Brassica rapa L."Plant Mol.Biol.. 40. 659-668 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Masao Watanabe: "Molecular biology of self-incompatibility in Brassica species."Plant Biotechnol.. 16. 263-272 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Naoko Kai: "Sequence comparisons among the Brassica S multigene members dispersing in an S9 genome."Mol.Gen.Genet.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hiroshi Shiba: "A pollen coat protein, SP11/SCR, determines the Pollen specificity in the Pollen self-incompatibility of Brassica."Plant Physiol.. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Go Suzuki: "Genomic organization of the S locus:Identiftcation and characterzation of genes in SLG/SRK region of S9 haplotype of Brassica campestris (syn.rapa)"Genetics. 153. 391-400 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Megumi Iwano: "X-ray micronalysis of papillar cells and pollen grains in the pollination process in Brassica using variable-pressure scanning electron microsope"J.Electron Microsc.. 48. 101-109 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Masao Watanabe: "Two anther-expressed genes downstream of SLG9:Identification of a novel S-linked gene spwcifically expressed in anthers at the uninucleate stage of Brassica campestris (syn.rapa) L."Sex.Plant Reprod.. 12. 127-134 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Seiji Takayama: "The pollen determinant of self-incompatibility in Brassica campestres"Proc.Natl.Acad.Sci.USA. 97. 1920-1925 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takeshi Takasaki: "The S receptor kinase determines self-incompatibility in Brassica stigma"Nature. 403. 913-916 (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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