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Analyses of action of a fruit component on the proliferation, invasion and metastasis of cancer cells and on cancerous hyperlipidemia.

Research Project

Project/Area Number 11460058
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 食品科学・栄養科学
Research InstitutionTOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY

Principal Investigator

YAGASAKI Kazumi  Tokyo University of Agriculture and Technology, Department of Applied Biological Science, Professor, 農学部, 教授 (20166474)

Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥12,100,000 (Direct Cost: ¥12,100,000)
Fiscal Year 2001: ¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2000: ¥4,500,000 (Direct Cost: ¥4,500,000)
Fiscal Year 1999: ¥4,800,000 (Direct Cost: ¥4,800,000)
Keywordshepatoma cells / cancer metastasis / cancer cell invasion / cancerous hyperlipidemia / resveratrol / reactive oxygen species / anti-oxidation / prostaglandin / 癌増殖 / 癌細胞浸潤 / 悪液質 / 果実成分 / 果実部分 / 肝癌増殖
Research Abstract

Effect of a fruit component, resveratrol, on the proliferation, invasion and metastasis of hepatoma cells, AH1 09A, and on cancerous hyperlipidemia in the hepatoma-bearing rats was studied in vitro and in vivo. Resveratrol significantly inhibited the proliferation and invasion of AH 109A in vitro. This food component was orally given to fasted rats, blood was collected at appropriate time intervals after administration, serum was prepared and subjected to the in vitro proliferation and invasion assays. Serum obtained 1 hr after oral administration most strongly suppressed the invasion but serum obtained 3 hr after oral administration lost the suppressive effect. In contrast, sera failed to suppress the hepatoma proliferation at any time points. Resveratrol-loaded rat sera dose-dependently suppressed the invasion, while they exerted no influence on the hepatoma proliferation even at a high dose of 100 mg/100 g of body weight. Resveratrol and resveratrol-loaded serum canceled the reactive oxygen species (ROS)-induced increase in the invasive capacity by scavenging ROS. The inhibitory activity of resveratrol on the invasion of the hepatoma cells was canceled by addition of prostaglandin (PG) E_2 to experimental media, indicating a possible involvement of PG in the invasion of AH109A. Reveratro dose-dependently decreased the hepatoma-induced hyperlipidemia and serum lipid peroxide concentration in hepatoma-bearing rats.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Y.Kozuki, Y.Miura, K.Yagasaki: "Resveratrol suppresses hepatoma cell invasion independently of its antiproliferative activity"Cancer Lett.. 167(2). 151-156 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.Kozuki, Y.Miura, K.Yagasaki: "Suppression of hepatoma cell invasion in culture by resveratrol and its modes of action"Animal Cell Technology : Basic & Applied Aspects. 12. 389-393 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.Kozuki, Y.Miura, K.Yagsaki: "Inhibitory effect of resveratrol, 3,5,4^1-trihydroxy-trans-stilbene, on the proliferation and invasion of hepatoma cells in culture"Polyphenol Communications 2000. 2. 369-370 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 矢ケ崎一三, 三浦 豊: "食品機能と抗がん作用"臨床栄養. 97(7). 831-839 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 矢ケ崎一三: "天然色素・ポリフェノール類の食理学"FFIジャーナル. 197. 7-12 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 矢ケ崎一三: "アミノ酸とくに非必須アミノ酸の薬理作用"臨床栄養. 100(2). 161-166 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y. Kozuki, Y. Miura, K. Yagasaki: "Resveratrol suppresses hepatoma cell invasion independently of its anti- proliterative activity"Cancer Lett. 167(2). 151-156 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y. Kozuki, Y. Miura, K. Yagasaki: "Suppression of hepatoma cell invasion in culture by resveratrol and its modes of action. In Animal Cell Technology : Basic & Applied Aspects, Vol. 12, Ed. By S. Shirahata, K. Teruya and Y. Katakura"Kluwer Academic Publishers, Dordrecht, The Netherlands. 389-393 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y. Kozuki,Y. Miura, K. Yagasak: "Inhibitory effect of resveratrol, 3,5,4'-trihydroxy-trans-stilbene, on the proliferation and invasion of hepatoma cells in culture"Polyphenol Communications 2000. 2. 369-370 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y. Kozuki, Y. Miura, K. Yagasaki: "Effects of food factors with antioxidative activities on proliferation and invasion of hepatoma cells in culture and on hypercholesterolemia in hepatoma-bearing rats."2nd International Conference on Food Factors (2nd ICoFF), Kyoto, December 13-17, 1999. Abstracts of papers. 171.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y. Kozuki, Y. Miura, K.Yagasaki: "Effects of food factors with antioxidative activities on the invasion of hepatoma cells co-cultured with mesothelial cells (mini-symposium)"8th Asian Congress of Nutrition, Seoul, August 29-September 2, 1999. Abstracts of papers. 221.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Yagasaki, T.Nihei, Y. Kozuki, Y. Miura: "Inhibitory effects of resveratrol, a polyphenolic component with antioxidative activity in grapes, inflammation-related symptoms in rats"17th International Congress of Nutrition, Vienna Austria, August 27-31, 2001. Abstracts of papers. 271.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Yaeasaki: "Anti-cancer activity and functional food factors : To stop endless proliferation and spreading of cancer cells. In Symposium 1 "Cancer prevention in northeast Asia""3rd International Conference of the Asia Pacific Clinical Nutrition Society, Osaka, March 10-1 3, 2002. Abstracts of Papers. S.32.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Yagasaki, Y.Miura/: "Food functionality and anti-cancer activity (In Japanese)"Jpn. J. Clin. Nutr. 97(7). 831-839 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Yagasaki: "Pharmacology of natural pigments and polyphenols (In Japanese)"FFIJ. No. 195. 7-12 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] K. Yagasaki: "Pharmacological action of nonessential amino acids (In Japanese)"Jpn, J. Clin. Nutr. 100(2). 161-166 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Y.Kozuki, Y.Miura, K.Yagasaki: "Resveratrol suppresses hepatoma cell invasion indepedently of its anti-proliferative activity"Cancer Lett., 167(2), 151-156, 2001.. 167(2). 151-156 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Kozuki, Y.Miura, K.Yagasaki: "Suppression of hepatoma cell invasion in culture by resveratrol and its modes of action"Animal Cell Technology: Basic & Applied Aspects. 12. 389-393 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Kozuki, Y.Miura, K.Yagasaki: "Inhibitory effect of resveratrol, 3, 5, 4'-trihydroxy-trans-stilbene, on the proliferation and invasion of hepatoma cells in culuture"Polyphenol Communications 2000. 2. 369-370 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 矢ケ崎 一三, 三浦 豊: "食品機能と抗がん作用"臨床栄養、97(7), 831-839, 2000.. 97(7). 831-839 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] 矢ケ崎 一三: "天然色素・ポリフェノール類の食理学"FFIジャーナル、No.195, 7-12, 2001.. 197. 7-12 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 矢ケ崎 一三: "アミノ酸とくに非必須アミノ酸の薬理作用"臨床栄養、100(2), 161-166, 2002.. 100(2). 161-166 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] Y.Kozuki: "Resveratrol suppresses hepatoma cell invasion independently of its antiproliferative activity."Cancer Lett.. (in press.). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Kozuki: "Suppression of hepatoma cell invasion in culture by resveratrol and its modes of action."Animal Cell Technology : Basic & Applied Aspects,. 12(in press,). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Kozuki: "Inhibitory effect of resveratrol, 3, 5, 4'-trihydroxy-trans-stilbene, on the proliferation and invasion of hepatoma cells in culture."Polyphenol Communications 2000.. 2. 369-370 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 矢ヶ崎一三: "栄養・食糧学研究最近の動向-In VivoおよびIn Vitro病態モデルによる食事処方と食品因子の腎炎および癌に対する有効性の評価."日本栄養・食糧学会誌. 53(3). 123-129 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 矢ヶ崎一三: "食品機能と抗がん作用."臨床栄養. 97(7). 831-839 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Y.Kozuki: "Effects of food factors with antioxidative activities on proliferation and invasion of hepatoma cells in culture and on bypercholesterolemia in hepatoma-bearing rats. "2nd International Conference on Food Factors (2nd ICoFF). Abstracts of papers. 171 (1999)

    • Related Report
      2000 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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